A Prevalence study of Entamoeba spp. in Basrah Province using Different  Detection Methods

Al-Hilfy, Athraa Abd-ulameer; Al-Malak, Maha Khalil; Al-Tomah, Muslim Abd-ulrahman

doi:10.21123/bsj.2021.18.4.1163

Cited by 5 publications

(26 citation statements)

References 24 publications

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“…Se usa un líquido de alta densidad y los elementos de interés flotaran en este. (21) La modificación de la técnica es más usada, donde se omite la centrifugación de la muestra y en dos fases de sedimentación se obtiene al parásito que se desea estudiar en un medio con sulfato de cinc. (15,21) 91 % 83 % Método estandarizado y de fácil realización 21 .…”

Section: Técnica De Faustunclassified

“…Los elementos para identificar se obtienen en un medio limpio que permite examinar la lámina de forma rápida. (21) Existen problemas cuando se manipulan muestras con gran contenido de grasas. (21) Es necesario usar una muestra con concentraciones de parásitos conocidos (control positivo).…”

Section: Técnica De Faustunclassified

“…(21) Existen problemas cuando se manipulan muestras con gran contenido de grasas. (21) Es necesario usar una muestra con concentraciones de parásitos conocidos (control positivo). (21) Prolongada exposición con sulfato de cinc puede deformar a los quistes.…”

Section: Técnica De Faustunclassified

“…(21) Es necesario usar una muestra con concentraciones de parásitos conocidos (control positivo). (21) Prolongada exposición con sulfato de cinc puede deformar a los quistes. (21) Procedimiento: valor estimado: 1500,0USD (21)…”

Section: Técnica De Faustunclassified

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Update on diagnostic techniques for Amebiasis

Garcés Curay,

Pérez Laborde

2024

Salud, Ciencia y Tecnología

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La amebiasis es una infección intestinal prevalente en todo el mundo, siendo la población de países subdesarrollados y tropicales los más afectados. La enfermedad es provocada por el género Entamoeba, se considera a Entamoeba histolytica la única especie patógena para el ser humano, afectando el intestino, pulmón, cerebro e hígado con la formación de abscesos. Se consideran que los datos de prevalencia e incidencia de amebiasis están sobreestimados, debido a que tradicionalmente la epidemiología de la enfermedad se ha estudiado mediante observación directa por microscopía, técnica que no facilita diferenciar a E. histolytica de otras amebas morfológicamente similares y no patógenas. Para identificar la tasa real de infección de amebiasis se han implementado técnicas y métodos que elevan moderadamente la especificidad diagnóstica de amebiasis, como las técnicas de concentración (Faust y Ritchie), tinciones, cultivos y exámenes de sangre, estos métodos no ha logrado resolver esta problemática de salud pública. La llegada de la tecnología los laboratorios han logrado implementar técnicas que identifiquen a microorganismos a nivel molecular utilizando técnicas como: Polymerase chain recation (PCR) o Loop-mediated isothermal amplification (LAMP), cuya tasa de especificidad están cerca al 100%, las cuales son consideradas como técnicas novedosas cuyo objetivo es actualizar el proceso diagnóstico de amebiasis.

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Section: Técnica De Faustunclassified

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Update on diagnostic techniques for Amebiasis

Garcés Curay,

Pérez Laborde

2024

Salud, Ciencia y Tecnología

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“…Molecular studies have shown that the prevalence of E. dispar is 10 times higher than that of E. histolytica worldwide [ 12 , 13 ]. Also, precise differentiation and identification of the pathogenic and non-pathogenic species and strains would be of primary importance from a therapeutic point of view [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Frequency of Entamoeba Complex in Individuals Referred to the Medical Laboratories in Jahrom City, South of Iran

Jahromi

Taghipour

Solhjoo

et al. 2023

Journal of Parasitology Research

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This study aimed to determine the prevalence of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii, using microscopic and molecular methods in Jahrom city (Fars Province), south of Iran. Stool samples were collected from 360 outpatients referred to the medical laboratories in Jahrom city. Standard parasitological methods, including direct wet mount examination, formalin–ether sedimentation technique, and trichrome staining techniques, were used for detection of Entamoeba complex. Nested polymerase chain reaction (PCR) was used to identify the genus/species of Entamoeba. Entamoeba complex cysts were detected in 2.5% (9/360) of samples by microscopic methods, while 11 isolates were considered positive for the Entamoeba complex by nested PCR. Among them, 2 (18.18%) out of 11 samples were E. histolytica, 5 (45.45%) were E. dispar, and 4 (36.36%) were E. moshkovskii. Molecular positivity was more prevalent among females (4.16%), people living in rural areas (3.44%), and people over 60 years old (13.33%). Considering the clinical manifestations, the Entamoeba complex infection in patients with fever (7.69%), severe diarrhea (5.10%), and nausea (5%) was high. This study reported the presence of E. histolytica, E. dispar, and E. moshkovskii in Jahrom city. Therefore, we suggest more public health interventions in Jahrom city.

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Molecular identification of Entamoeba spp. in humans and cattle in Baghdad, Iraq

Al-Dabbagh,

Alseady,

Alhadad

2024

Vet World

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Background and Aim: A total of 10% of the global population succumbs to amoebiasis yearly, equating to 50,000–100,000 recorded fatalities. It is closely associated with contaminated food and water supplies due to human feces. The disease’s pathophysiology remains a subject of ongoing debate among experts. Some experts attribute the role of the host’s conditions, parasite species and strain, and infection intensity in eliciting clinical symptoms. The aim of this study was to perform molecular identification of Entamoeba species isolated from humans and cattle. Materials and Methods: Stool samples from three hundred patients and one hundred cattle were collected from different regions, age groups, and sexes in Baghdad for microscopic examination. One hundred randomly chosen patient and cattle stool samples underwent microscopic examination and conventional polymerase chain reaction (PCR) targeting the 18S rRNA gene. Phylogenetic tree analyses were performed for Entamoeba species identification. Results: The infection rate in humans differed significantly (p < 0.05) between age groups and genders, totaling 38%. The infection rate in cattle, determined by conventional PCR, differed significantly (p < 0.05) between age groups and genders, amounting to 58%. Ten PCR products with positive results were sequenced and deposited in GenBank database. Sequence analysis detected that Eight sequences belong to E. histolytica ( OM268853.1, OM268854.1, OM268855.1, OM268857.1, OM268858.1, OM268860.1, OM268861.1, and OM268862.1) and two sequences belong to E. dispar (OM268856.1 and OM268859.1) in humans, while 10 sequences (ON724165.1 to ON724174.1) belongs to E. histolytica in cattle. Conclusion: The increased susceptibility of cattle to E. histolytica suggests a considerable role in human infection and substantial public health risks. Further research should be conducted on the many virulence factors such as HM1:IMSS strain, cysteinprotease (Cp1), Gal/lectin, etc. of E. histolytica and E. dispar. Keywords: cattle, Entamoeba spp., human, phylogenetic, sequence analyses.

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A Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods

Cited by 5 publications

References 24 publications

Update on diagnostic techniques for Amebiasis

Update on diagnostic techniques for Amebiasis

Frequency of Entamoeba Complex in Individuals Referred to the Medical Laboratories in Jahrom City, South of Iran

Molecular identification of Entamoeba spp. in humans and cattle in Baghdad, Iraq

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