A previously unknown zinc finger protein, DST, regulates drought and salt tolerance in rice via stomatal aperture control

Huang, Xin‐Yuan; Chao, Dai‐Yin; Gao, Jinliang; Zhu, Meizhen; Shi, Min; Lin, Hong-Xuan

doi:10.1101/gad.1812409

Cited by 546 publications

(459 citation statements)

References 51 publications

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“…The transactivation activity assay was performed as described (39). To create pLer, pLerΔC225-340, pLerΔC205-340, pCvi, and pCviΔC205-224, the full-length coding sequence and the C terminus deletions of FSQ6/NAC089 based on the Ler and Cvi sequences were PCR amplified.…”

Section: Methodsmentioning

confidence: 99%

Fructose sensitivity is suppressed in Arabidopsis by the transcription factor ANAC089 lacking the membrane-bound domain

Wind

Shi

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

115

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In living organisms sugars not only provide energy and carbon skeletons but also act as evolutionarily conserved signaling molecules. The three major soluble sugars in plants are sucrose, glucose, and fructose. Information on plant glucose and sucrose signaling is available, but to date no fructose-specific signaling pathway has been reported. In this study, sugar repression of seedling development was used to study fructose sensitivity in the Landsberg erecta (Ler)/Cape Verde Islands (Cvi) recombinant inbred line population, and eight fructose-sensing quantitative trait loci (QTLs) (FSQ1-8) were mapped. Among them, FSQ6 was confirmed to be a fructose-specific QTL by analyzing near-isogenic lines in which Cvi genomic fragments were introgressed in the Ler background. These results indicate the existence of a fructose-specific signaling pathway in Arabidopsis. Further analysis demonstrated that the FSQ6-associated fructose-signaling pathway functions independently of the hexokinase1 (HXK1) glucose sensor. Remarkably, fructosespecific FSQ6 downstream signaling interacts with abscisic acid (ABA)-and ethylene-signaling pathways, similar to HXK1-dependent glucose signaling. The Cvi allele of FSQ6 acts as a suppressor of fructose signaling. The FSQ6 gene was identified using mapbased cloning approach, and FSQ6 was shown to encode the transcription factor gene Arabidopsis NAC (petunia No apical meristem and Arabidopsis transcription activation factor 1, 2 and Cup-shaped cotyledon 2) domain containing protein 89 (ANAC089). The Cvi allele of FSQ6/ANAC089 is a gain-of-function allele caused by a premature stop in the third exon of the gene. The truncated Cvi FSQ6/ ANAC089 protein lacks a membrane association domain that is present in ANAC089 proteins from other Arabidopsis accessions. As a result, Cvi FSQ6/ANAC089 is constitutively active as a transcription factor in the nucleus. I n plants, sugars provide the energy and carbon skeletons needed for growth and in addition act as crucial signaling molecules that affect growth, development, and response to the (a)biotic environment (1-3). Plant cells harbor sugar-sensing and -signaling systems that regulate the expression of thousands of genes and control the metabolic processes needed for growth (2-4). These sugar-response systems are known to interact with other signaling pathways, such as those for light, phytohormones, stress, and nutrients (1).The neutral sugars sucrose, glucose, and fructose are central to metabolism in plants and in other organisms as well. So far, detailed information is available only on glucose sensing, and it has been shown that the hexokinase 1 (HXK1) enzyme acts as a glucose sensor (5, 6). Sucrose-specific signaling also was demonstrated because the effect of sucrose cannot be mimicked by glucose and/or fructose (7), but so far no information on sucrosesensing systems is available. A signaling function for fructose has been proposed (8, 9), but no convincing experimental evidence on such fructose-specific signaling is available.In Arabidopsis ea...

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Section: Methodsmentioning

confidence: 99%

Fructose sensitivity is suppressed in Arabidopsis by the transcription factor ANAC089 lacking the membrane-bound domain

Wind

Shi

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

115

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“…The measurement of H 2 O 2 production was performed by extracting H 2 O 2 from leaves according to a previously described method (Huang et al, 2009) and quantifying the molecule with the Amplex Red Hydrogen Peroxidase Assay Kit (Molecular Probes, Invitrogen) according to the manufacturer's instructions. Catalase activity was carried out with a Catalase Assay Kit (Beyotime) according to the manufacturer's instructions.…”

Section: Quantitative Measurement Of H 2 O 2 Content and Catalase Actmentioning

confidence: 99%

Nitric Oxide and ProteinS-Nitrosylation Are Integral to Hydrogen Peroxide-Induced Leaf Cell Death in Rice

et al. 2011

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Nitric oxide (NO) is a key redox-active, small molecule involved in various aspects of plant growth and development. Here, we report the identification of an NO accumulation mutant, nitric oxide excess1 (noe1), in rice (Oryza sativa), the isolation of the corresponding gene, and the analysis of its role in NO-mediated leaf cell death. Map-based cloning revealed that NOE1 encoded a rice catalase, OsCATC. Furthermore, noe1 resulted in an increase of hydrogen peroxide (H 2 O 2 ) in the leaves, which consequently promoted NO production via the activation of nitrate reductase. The removal of excess NO reduced cell death in both leaves and suspension cultures derived from noe1 plants, implicating NO as an important endogenous mediator of H 2 O 2 -induced leaf cell death. Reduction of intracellular S-nitrosothiol (SNO) levels, generated by overexpression of rice S-nitrosoglutathione reductase gene (GSNOR1), which regulates global levels of protein S-nitrosylation, alleviated leaf cell death in noe1 plants. Thus, S-nitrosylation was also involved in light-dependent leaf cell death in noe1. Utilizing the biotin-switch assay, nanoliquid chromatography, and tandem mass spectrometry, S-nitrosylated proteins were identified in both wild-type and noe1 plants. NO targets identified only in noe1 plants included glyceraldehyde 3-phosphate dehydrogenase and thioredoxin, which have been reported to be involved in S-nitrosylation-regulated cell death in animals. Collectively, our data suggest that both NO and SNOs are important mediators in the process of H 2 O 2 -induced leaf cell death in rice.

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“…The mutant lines exhibiting significantly higher grain yield or higher tolerance to nutrient deficiency or abiotic stresses or other traits can be used for rice breeding by conventional backcrossing along with molecular marker-assisted selection (Jiang and Ramachandran, 2010;Sikora et al, 2011). EMS induced rice mutants were used to investigate the complex mechanism involved in water and salt stress tolerance (Huang et al, 2009;Zhou et al, 2013). The morphological, physiological and proteomic characterization of some EMS induced mutants showed more tolerance to abiotic stresses for example salt and heat in contrast to their respective wild type lines (Ghaffari et al, 2014;Poli et al, 2013;Nakhoda et al, 2012;Mithra et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

Yugandhar¹,

Basava²,

Subrahmanyam³

et al. 2017

Aust J Crop Sci

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Mutants are powerful genetic resources in plant breeding and functional genomics studies. Sixty seven stable ethyl methane sulphonate (EMS) induced rice mutants and the wild type parent Nagina 22 (N22) were characterized for plant height, tiller number, panicle number and grain yield under normal, low P field and alternate wet and dry (AWD) conditions in the same season. They were also genotyped with 44 SSR markers and four Pup1 (Phosphorus uptake1) gene specific markers. Genetic diversity was analysed by combining phenotype and marker data using Ward-MLM method. Single marker analysis showed significant association of four markers RM19696, RM263, RM3688 and RM1942 with grain yield in all three conditions. K-1, a Pup1 gene specific marker was significantly associated with tiller number only under low P conditions. The average dissimilarity between mutants was 0.86 and cophenetic correlation coefficient was 0.74. Six mutants were selected as gain-of-function mutants as they showed significantly higher grain yield in all three conditions, compared with N22. The selected mutants are an important resource for gene discovery for enhanced tolerance to low P and water stress conditions and associated markers can be useful in marker assisted selection.

show abstract

A previously unknown zinc finger protein, DST, regulates drought and salt tolerance in rice via stomatal aperture control

Cited by 546 publications

References 51 publications

Fructose sensitivity is suppressed in Arabidopsis by the transcription factor ANAC089 lacking the membrane-bound domain

Fructose sensitivity is suppressed in Arabidopsis by the transcription factor ANAC089 lacking the membrane-bound domain

Nitric Oxide and ProteinS-Nitrosylation Are Integral to Hydrogen Peroxide-Induced Leaf Cell Death in Rice

Identifying markers associated with yield traits in Nagina22 rice mutants grown in low phosphorus field or in alternate wet/dry conditions

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