A procedure for intravenous injection using external jugular vein in Mongolian gerbil (<i>Meriones unguiculatus</i>)

Palm, D Kakol; Hollander, P. H. C. den

doi:10.1258/002367707781282721

Cited by 6 publications

(1 citation statement)

References 3 publications

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“…A longitudinal incision of about 15 mm was made in the neck of the animal, just inside the right front leg. After careful removal of the connective tissue surrounding the jugular vein, the indicated drug was slowly (over 10 s) injected through the jugular vein using a 27-gauge needle, and the skin was sutured [ 9 ]. Drugs were diluted with saline, and the injection volume was adjusted to about 0.1 ml.…”

Section: Methodsmentioning

confidence: 99%

IV injection of polystyrene beads for mouse model of sepsis causes severe glomerular injury

et al. 2014

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BackgroundInfusion fluids may be contaminated with different types of particulates that are a potential health hazard. Particulates larger than microvessels may cause an embolism by mechanical blockage and inflammation; however, it has been reported that particulates smaller than capillary diameter are relatively safe. Against such a background, one report showed that polystyrene beads smaller than capillary diameter decreased tissue perfusion in ischemia–reperfusion injury. This report suggested that polystyrene beads from 1.5- to 6-μm diameter (dia.) may have unfavorable effects after pretreatment.Here, we investigated whether injection of polystyrene beads (3- and 6-μm dia.) as an artificial contaminant of intravenous fluid after lipopolysaccharide (LPS) injection affected mortality and organ damage in mice.MethodsMice were divided into four groups and injected: polystyrene beads only, LPS only, polystyrene beads 30 min after LPS, or saline. A survival study, histology, blood examination, and urine examination were performed.ResultsThe survival rate after LPS and polystyrene bead (6-μm dia.) injection was significantly lower than that of the other three groups. In the kidney sections, injured glomeruli were significantly higher with LPS and polystyrene bead injection than that of the other three groups. LPS and polystyrene bead injection decreased the glomerular filtration rate and led to renal failure. Inflammatory reactions induced with LPS were not significantly different between with or without polystyrene beads. Polystyrene beads were found in urine after LPS and polystyrene bead injection.ConclusionsInjection of polystyrene beads after LPS injection enhanced glomerular structural injury and caused renal function injury in a mouse sepsis model.

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Section: Methodsmentioning

confidence: 99%