A procedure to extract functional isolated mitochondria from small-sized human atrial samples. Application to obesity with a partial characterisation of the organelles

Leger, Thibault; Jouve, Chrystèle; Patrac, Véronique; Batel, V.; Bouvier, Damien; Sapin, Vincent; Miguel, Bruno; Demaison, Luc; Azarnoush, Kasra

doi:10.1016/j.freeradbiomed.2020.04.006

Cited by 2 publications

(3 citation statements)

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“…Further supporting a plausible increase in intracellular and matrix calcium, we have previously observed a stimulation of mitochondrial energy production in the same atrial human samples than those used for the present study [18]. A physiological elevation of matrix calcium stimulates oxidative phosphorylation [20].…”

Section: Stimulation Of Cardiac Mechanical Work Pufa Profile Of Cardi...supporting

confidence: 84%

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An experimental model of western diet in female Wistar rats leads to cardiac hypoxia related to a stimulated contractility

Rigaudière,

Jouve,

Capel

et al. 2024

J Physiol Biochem

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Previous studies in Western diet (WD)-fed male rats have highlighted a link between the stimulation of cardiac contractility, mitochondrial adaptations and a pro-inflammatory fatty acid profile of phospholipids in the heart. Our objectives were to determine 1) if WD-fed female Wistar rats and obese humans display a similar pro-inflammatory profile in their cardiac phospholipids and 2) if this lipid profile is associated with deleterious effects on the heart of the female rodents. Female Wistar rats were fed WD for 5 weeks or a laboratory chow as a control. Ionic homeostasis, redox status, inflammation markers, and fatty acid composition of phospholipids were analysed in the heart., WD increased the abdominal fat mass without modifying the body weight of female rats. As previously found in males, a WD induced a shift in membrane fatty acid composition toward a pro-inflammatory profile in the female rats, but not in obese humans. It was associated with an increased COX2 expression suggesting an increased pro-inflammatory eicosanoid production. Signs of increased intracellular calcium strongly supported a stimulation of cardiac contractility without any induction of apoptosis. The heart of WD-fed rats exhibited a hypoxic state as a higher HIF1- expression was reported. The expressions of antioxidant enzymes were increased, but the redox reserves against reactive oxygen species were lowered. In conclusion, as previously observed in males, we suppose that cardiac abnormalities are magnified with severe obesity in female rats, leading to hypoxia and intense oxidative stress which could ultimately induce cell death and heart failure. Key points :-WD increases myocardial calcium and induces cardiac hypoxia probably through a stimulation of contractility in female rats -These phenomena are associated with a strong decrease in the GSH/GSSG ratio -The capacity to fight against oxidative stress-induced cardiomyocyte death is thus reduced by WD

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Section: Stimulation Of Cardiac Mechanical Work Pufa Profile Of Cardi...supporting

confidence: 84%

“…The clinical characteristics of the lean and obese patients involved in the present study are presented in a previous publication [18].…”

Section: Collection Of Human Atrial Biopsiesmentioning

confidence: 99%

An experimental model of western diet in female Wistar rats leads to cardiac hypoxia related to a stimulated contractility

Rigaudière,

Jouve,

Capel

et al. 2024

J Physiol Biochem

Self Cite

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show abstract

“…Obesity is a risk factor for the development of type 2 diabetes (T2D), which is associated with cardiovascular diseases [1]. Although moderate obesity improves the function of cardiac mitochondria in animals [2] and humans (BMI = 32) [3], diabetes mellitus strongly deteriorates the activity of these organelles [4] and precipitates the myocardium toward heart failure [5]. The deleterious action of T2D on mitochondrial function seems to result from increased oxidative stress [6].…”

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confidence: 99%