A profile of Drosophila species' enzymes assayed by electrophoresis. I. Number of alleles, heterozygosities, and linkage disequilibrium in glucose-metabolizing systems and some other enzymes

Kojima, Ken-ichi; Gillespie, John H.; Yn, Toari

doi:10.1007/bf00486100

Cited by 239 publications

(112 citation statements)

References 9 publications

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“…This did not affect the test for independence between populations in phenotype frequencies, however, and within Hawaii and between islands tests proved insignificant. Although we find three alleles, Kojima et al (1970) report only two in their study.…”

Section: Drosophila Simulans Variabilitycontrasting

confidence: 60%

“…At the EST-6 locus (Table 3) we find only two alleles compared to four found by Kojima et al (1970). The two fast migrating alleles appear to be missing in -4 - • x 2 = 7.64, P < o.o1…”

Section: Drosophila Simulans Variabilitymentioning

confidence: 73%

“…First observed by Lewontin and Hubby (1966), this trend seems supported by the findings of O'Brien and Macintyre (1969) and Berger (1970). The latter two studies have found low or non-existent electrophoretic variation in the chromosomally monomorphic species Drosophila simulans, although Kojima et al (1970) found evidence to the contrary.…”

Section: Introductionmentioning

confidence: 95%

“…A chi-square test for independence between all s~mples proved insignificant between all samples and each population met Hardy-Weinberg expectations. Kojima et al (1970) report four alleles for this enzyme. At the PGM locus~ (Table 2) the TM population was not in HardyWeinberg equilibrium.…”

Section: Drosophila Simulans Variabilitymentioning

confidence: 99%

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Electrophoretic variability in island populations of Drosophila simulans and Drosophila immigrans

1976

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Section: Drosophila Simulans Variabilitycontrasting

confidence: 60%

“…At the EST-6 locus (Table 3) we find only two alleles compared to four found by Kojima et al (1970). The two fast migrating alleles appear to be missing in -4 - • x 2 = 7.64, P < o.o1…”

Section: Drosophila Simulans Variabilitymentioning

confidence: 73%

Section: Introductionmentioning

confidence: 95%

Section: Drosophila Simulans Variabilitymentioning

confidence: 99%

See 2 more Smart Citations

Electrophoretic variability in island populations of Drosophila simulans and Drosophila immigrans

1976

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“…These single chromosomes may thus be made homozygous before establishing their allelic content (e.g. Kojima et al, 1970;Mukai et al, 1971). An equivalent procedure is to test cross individuals against a marker stock.…”

Section: Introductionmentioning

confidence: 99%

Estimation of linkage disequilibrium in randomly mating populations

Hill¹

1974

Heredity

572

396

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SUMMARYThe degree of linkage disequilibrium, D, between two loci can be estimated by maximum likelihood from the frequency of diploid genotypes in a sample from a random-mating population. Haploid genotypes can be identified directly in some species from a sample of chromosomes extracted from the population and made homozygous, or by test crossing. The maximum likelihood estimators of D are described, with examples, for both methods, including the cases where both loci are codominant and one or both are dominant.The efficiencies of the methods are compared when D = 0; If both loci are codominant the estimate of D has the same variance,from a sample of .N identified diploids as from N identified haploid types, where p and q are the gene frequencies; therefore the diploid method is more efficient in practice since less labour is required. With dominance at either locus V(O) is lower for samples of the same size using the haploid method if the dominant alleles are at high frequency.

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Quantification of silver‐stained proteins resolved by two‐dimensional electrophoresis: Genetic variability as related to abundance and solubility in two maize lines

Damerval

1994

Electrophoresis

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Relative abundance and solubility of proteins from etiolated coleoptiles of maize were investigated using two-dimensional electrophoresis (2-D PAGE). Automatic quantification of silver-stained polypeptides on replicate 2-D gels made it possible to test the linearity of the relationship between spot integrated optical density and protein amount in the range from 15 micrograms to 135 micrograms per gel, in two inbred lines. A linear response was found for more than 60% of the spots in each genotype. When a linear response was found in both lines for a given spot, the slope values were similar in 94% of the cases, indicating the reliability of silver staining for polypeptide quantification. The parameters of the curves allowed the definition of protein classes of different abundances that could be compared for genetic variability between the two lines. From a comparison between standard 2-D patterns (trichloroacetic acid-acetone extracted proteins) and patterns obtained from Tris buffer extracted proteins, it appeared that 92% of the proteins visualized in the standard gels were soluble. No difference in genetic variability, either qualitative or quantitative, was evidenced between the various classes of abundance, or between soluble and insoluble proteins.

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A profile of Drosophila species' enzymes assayed by electrophoresis. I. Number of alleles, heterozygosities, and linkage disequilibrium in glucose-metabolizing systems and some other enzymes

Cited by 239 publications

References 9 publications

Electrophoretic variability in island populations of Drosophila simulans and Drosophila immigrans

Electrophoretic variability in island populations of Drosophila simulans and Drosophila immigrans

Estimation of linkage disequilibrium in randomly mating populations

Quantification of silver‐stained proteins resolved by two‐dimensional electrophoresis: Genetic variability as related to abundance and solubility in two maize lines

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