A Profile of Volatile Organic Compounds in Breath Discriminates COPD Patients from Controls.

Berkel, J van; Dallinga, Jan W.; Møller, Grith; Godschalk, Roger; Moonen, E.J.C.; Wouters, Emiel F.M.; Schooten, F van

doi:10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a3774

Cited by 27 publications

(60 citation statements)

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“…Around 40% of these hydrocarbons were less prevalent in the exhaled breath of children with recurrent wheeze compared with children without wheezing symptoms. This is comparable to our previous findings in COPD patients and children with asthma [5,8]. The complicated biological equilibrium of formation and removal of VOCs in the human body might be a possible explanation for this finding.…”

Section: Discussionsupporting

confidence: 91%

“…However, this approach is vulnerable to mistakes and improper identification of compounds can hamper the quality of databases. Our innovative procedure that matches compounds on the basis of the raw mass spectra and retention time creates a more reliable database [8].…”

Section: Discussionmentioning

confidence: 99%

“…Despite this advanced statistical technique, the results are still easy to interpret. Other modelling techniques that were previously applied to VOC data included discriminant analysis (either with or without principal component analysis as a pre-processing tool) [5][6][7]18], support vector machine analysis [8,19] and fuzzy logic models [20]. Currently, there is no consensus about the optimal statistical technique to analyse VOCs.…”

Section: Discussionmentioning

confidence: 99%

“…In adults and older children, the study of metabolomics using exhaled VOCs is known to be a safe and noninvasive procedure to evaluate ongoing processes of inflammation and oxidative stress [3,5]. Profiling exhaled VOCs has been successfully applied in the diagnosis of inflammatory lung diseases, such as cystic fibrosis [6], asthma [5] and chronic obstructive pulmonary disease (COPD) [7,8].…”

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confidence: 99%

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Exhaled breath profiling in diagnosing wheezy preschool children

Kant

Berkel²,

Jöbsis

et al. 2012

Eur Respir J

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Although wheeze is common in preschool children, the underlying pathophysiology has not yet been disentangled. Volatile organic compounds (VOCs) in exhaled breath may serve as noninvasive markers of early wheeze. We aimed to assess the feasibility of VOC collection in preschool children, and to study whether a VOC profile can differentiate between children with and without recurrent wheeze.We included children (mean (range) age 3.3 (1.9-4.5) yrs) with (n5202) and without (n550) recurrent wheeze. Exhaled VOCs were analysed by gas chromatography-time-of-flight mass spectrometry. VOC profiles were generated by ANOVA simultaneous component analysis (ASCA) and sparse logistic regression (SLR).Exhaled breath collection was possible in 98% of the children. In total, 913 different VOCs were detected. The signal-to-noise ratio improved after correction for age, sex and season using ASCA pre-processing. An SLR model with 28 VOCs correctly classified 83% of the children (84% sensitivity, 80% specificity). After six-fold cross-validation, 73% were correctly classified (79% sensitivity, 50% specificity).Assessment of VOCs in exhaled breath is feasible in young children. VOC profiles are able to distinguish children with and without recurrent wheeze with a reasonable accuracy. This proof of principle paves the way for additional research on VOCs in preschool wheezing.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Exhaled breath profiling in diagnosing wheezy preschool children

Kant

Berkel²,

Jöbsis

et al. 2012

Eur Respir J

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“…Decane and the 6-ethyl-2-methyl decane, the compounds that positively correlated with COPD, are alkanes that belong to the broader class of hydrocarbons. In previous studies, the analysis of exhaled air with the GC-MS has identified increased levels of decane [31] and its derivatives [32] in subjects with COPD [33] and lung cancer [34], compared with healthy subjects. In our patients, the levels of decane and 6-ethyl-2-methyl decane were increased in frequent exacerbators COPD [35,36] compared to non-frequent exacerbators, and in the latter group compared to healthy subjects.…”

Section: Discussionmentioning

confidence: 99%

Analysis of exhaled breath fingerprints and volatile organic compounds in COPD

et al. 2015

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Background: Exhaled air contains many volatile organic compounds (VOCs) produced during human metabolic processes, in both healthy and pathological conditions. Analysis of breath allows studying the modifications of the profile of the exhaled VOCs due to different disease states, including chronic obstructive pulmonary disease (COPD). The early diagnosis of COPD is complicated and the identification of specific metabolic profiles of exhaled air may provide useful indication to better identify the disease. The aim of our study was to characterize the specific exhaled VOCs by means of the electronic nose and by solid phase micro-extraction associated to gas chromatography-mass spectrometry (SPME GC-MS). Methods: Exhaled air was collected and measured in 34 subjects, 7 healthy and 27 former smokers affected by COPD (GOLD 1-4).Results: The signals of the electronic nose sensors were higher in COPD patients with respect to controls, and allowed to accurately classify the studied subjects in healthy or COPD. GC-MS analysis identified 37 VOCs, nine of which were significantly correlated with COPD. In particular the concentration of two of these were positively correlated whereas seven were negatively correlated with COPD. The partial least squares discriminant analysis (PLS-DA) carried out with these nine VOCs produced a significant predictive model of disease. Conclusions: This study shows that COPD patients exhibit qualitative and quantitative differences in the chemical compositions of exhale. These differences are detectable both by the GC-MS and the six-sensor e-nose. The use of electronic nose may represent a suitable, non-invasive diagnostic tool for characterization of COPD.

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Ultrafast gas chromatography coupled to electronic nose to identify volatile biomarkers in exhaled breath from chronic obstructive pulmonary disease patients: A pilot study

Rodríguez-Aguilar

Ramírez-García

Ilizaliturri-Hernández

et al. 2019

Biomedical Chromatography

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An analytical method to identify volatile organic compounds (VOCs) in the exhaled breath from patients with a diagnosis of chronic obstructive pulmonary disease (COPD) using a ultrafast gas chromatography system equipped with an electronic nose detector (FGC eNose) has been developed. A prospective study was performed in 23 COPD patients and 33 healthy volunteers; exhalation breathing tests were performed with Tedlar bags. Each sample was analyzed by FCG eNose and the identification of VOCs was based on the Kovats index. Raw data were reduced by principal component analysis (PCA) and canonical discriminant analysis [canonical analysis of principal coordinates (CAP)]. The FCG eNose technology was able to identify 17 VOCs that distinguish COPD patients from healthy volunteers. At all stages of PCA and CAP the discrimination between groups was obvious. Chemical prints were correctly classified up to 82.2%, and were matched with 78.9% of the VOCs detected in the exhaled breath samples. Receiver operating characteristic curve analysis indicated the sensitivity and specificity to be 96% and 91%, respectively. This pilot study demonstrates that FGC eNose is a useful tool to identify VOCs as biomarkers in exhaled breath from COPD patients. Further studies should be performed to enhance the clinical relevance of this quick and ease methodology for COPD diagnosis.

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A Profile of Volatile Organic Compounds in Breath Discriminates COPD Patients from Controls.

Cited by 27 publications

References 0 publications

Exhaled breath profiling in diagnosing wheezy preschool children

Exhaled breath profiling in diagnosing wheezy preschool children

Analysis of exhaled breath fingerprints and volatile organic compounds in COPD

Ultrafast gas chromatography coupled to electronic nose to identify volatile biomarkers in exhaled breath from chronic obstructive pulmonary disease patients: A pilot study

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