A Program for Iron Economy during Deficiency Targets Specific Fe Proteins

Hantzis, Laura J.; Kroh, Gretchen E; Jahn, Courtney E.; Cantrell, Michael; Peers, Graham; Pilon, Marinus; Ravet, Karl

doi:10.1104/pp.17.01497

Cited by 72 publications

(83 citation statements)

References 86 publications

(121 reference statements)

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“…Fe deficiency decreases the Fe content of chloroplasts. Limitation in the availability of Fe leads to a significant decrease in the amount of Fe-S cluster biogenesis and sulphate assimilation pathway components, ascorbate peroxidase, cytochrome b 6 /f complex units PetA and PetC, photosystem I component PsaC and PsaD and soluble ferredoxin (Hantzis et al 2018). Fe deficiency also induces significant alterations in the PSII complex organization (Basa et al 2014).…”

Section: Electronic Supplementary Materialsmentioning

confidence: 99%

The developmental and iron nutritional pattern of PIC1 and NiCo does not support their interdependent and exclusive collaboration in chloroplast iron transport in Brassica napus

Pham

Pólya

Müller

et al. 2020

Planta

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Main conclusion The accumulation of NiCo following the termination of the accumulation of iron in chloroplast suggests that NiCo is not solely involved in iron uptake processes of chloroplasts.Abstract Chloroplast iron (Fe) uptake is thought to be operated by a complex containing permease in chloroplast 1 (PIC1) and nickel-cobalt transporter (NiCo) proteins, whereas the role of other Fe homeostasis-related transporters such as multiple antibiotic resistance protein 1 (MAR1) is less characterized. Although pieces of information exist on the regulation of chloroplast Fe uptake, including the effect of plant Fe homeostasis, the whole system has not been revealed in detail yet. Thus, we aimed to follow leaf development-scale changes in the chloroplast Fe uptake components PIC1, NiCo and MAR1 under deficient, optimal and supraoptimal Fe nutrition using Brassica napus as model. Fe deficiency decreased both the photosynthetic activity and the Fe content of plastids. Supraoptimal Fe nutrition caused neither Fe accumulation in chloroplasts nor any toxic effects, thus only fully saturated the need for Fe in the leaves. In parallel with the increasing Fe supply of plants and ageing of the leaves, the expression of BnPIC1 was tendentiously repressed. Though transcript and protein amount of BnNiCo tendentiously increased during leaf development, it was even markedly upregulated in ageing leaves. The relative transcript amount of BnMAR1 increased mainly in ageing leaves facing Fe deficiency. Taken together chloroplast physiology, Fe content and transcript amount data, the exclusive participation of NiCo in the chloroplast Fe uptake is not supported. Saturation of the Fe requirement of chloroplasts seems to be linked to the delay of decomposing the photosynthetic apparatus and keeping chloroplast Fe homeostasis in a rather constant status together with a supressed Fe uptake machinery.

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Section: Electronic Supplementary Materialsmentioning

confidence: 99%

The developmental and iron nutritional pattern of PIC1 and NiCo does not support their interdependent and exclusive collaboration in chloroplast iron transport in Brassica napus

Pham

Pólya

Müller

et al. 2020

Planta

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“…WT, Wild type. active cells (Hantzis et al, 2018). Interestingly, Fe deficiency responses were sustained even after the seedling was able to acquire Fe from the medium to restore growth and photosynthetic function.…”

Section: Cell Expansion and Nutrient Transport Are Actively Restrictementioning

confidence: 99%

Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds

et al. 2018

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During seed germination, iron (Fe) stored in vacuoles is exported by the redundant NRAMP3 and NRAMP4 transporter proteins. A double mutant is unable to mobilize Fe stores and does not develop in the absence of external Fe. We used RNA sequencing to compare gene expression in and wild type during germination and early seedling development. Even though sufficient Fe was supplied, the Fe-responsive transcription factors ,, , and and their downstream targets and mediating Fe uptake were strongly upregulated in the mutant. Activation of the Fe deficiency response was confirmed by increased ferric chelate reductase activity in the mutant. At early stages, genes important for chloroplast redox control ( and ), Fe homeostasis ( and ), and chlorophyll metabolism ( and ) were downregulated, indicating limited Fe availability in plastids. In contrast, expression of, encoding a ferric reductase involved in Fe import into the mitochondria, was maintained, and Fe-dependent enzymes in the mitochondria were unaffected in Together, these data show that a failure to mobilize Fe stores during germination triggered Fe deficiency responses and strongly affected plastids, but not mitochondria.

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“…This is of particular importance for reactive elements such as iron and copper, which are required in relatively small amounts (micromolar range), but can become toxic at a relatively low level (submillimolar ranges). It has become clear in recent years that there is active crosstalk between networks regulating the uptake and use of nutrients [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, under sulfur deficiency, the release of phytosiderophores was reduced; however, when barley plants were resupplied with sulfate, the release of phytosiderophores was enhanced [42]. In dicots, sulfur deficiency conditions render plants unable to fully induce their iron uptake machinery, while under iron limitation, the sulfite reduction is stopped [6,7]. Transcriptomic analyses of 5-week iron starved Arabidopsis roots indicated a downregulation of genes of sulfate assimilation [43].…”

Section: Introductionmentioning

confidence: 99%

The Role of Selective Protein Degradation in the Regulation of Iron and Sulfur Homeostasis in Plants

Wawrzyńska

Sirko

2020

IJMS

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Plants are able to synthesize all essential metabolites from minerals, water, and light to complete their life cycle. This plasticity comes at a high energy cost, and therefore, plants need to tightly allocate resources in order to control their economy. Being sessile, plants can only adapt to fluctuating environmental conditions, relying on quality control mechanisms. The remodeling of cellular components plays a crucial role, not only in response to stress, but also in normal plant development. Dynamic protein turnover is ensured through regulated protein synthesis and degradation processes. To effectively target a wide range of proteins for degradation, plants utilize two mechanistically-distinct, but largely complementary systems: the 26S proteasome and the autophagy. As both proteasomaland autophagy-mediated protein degradation use ubiquitin as an essential signal of substrate recognition, they share ubiquitin conjugation machinery and downstream ubiquitin recognition modules. Recent progress has been made in understanding the cellular homeostasis of iron and sulfur metabolisms individually, and growing evidence indicates that complex crosstalk exists between iron and sulfur networks. In this review, we highlight the latest publications elucidating the role of selective protein degradation in the control of iron and sulfur metabolism during plant development, as well as environmental stresses. are present at certain concentrations, i.e., when the demand meets the supply. Below such levels, the demand for a nutrient exceeds the supply, so plant growth is ultimately limited by a nutritional deficiency. Conversely, an excess of any nutrient is toxic to plants and also negatively affects plant growth and development. Plants can therefore experience transitions between deficiency or toxicity zones due to changes in the availability of nutrients and growth requirements, but they can return to homeostasis so long as the response is adequate and sufficient. Such adjustments may require time, but they are crucial for plant adaptation. A significant part of this adaptation relies on selective protein degradation (Figure 1). Int. J. Mol. Sci. 2020, 21, x FOR PEER REVIEW 2 of 21 nutrients are present at certain concentrations, i.e., when the demand meets the supply. Below such levels, the demand for a nutrient exceeds the supply, so plant growth is ultimately limited by a nutritional deficiency. Conversely, an excess of any nutrient is toxic to plants and also negatively affects plant growth and development. Plants can therefore experience transitions between deficiency or toxicity zones due to changes in the availability of nutrients and growth requirements, but they can return to homeostasis so long as the response is adequate and sufficient. Such adjustments may require time, but they are crucial for plant adaptation. A significant part of this adaptation relies on selective protein degradation ( Figure 1). Figure 1.Regulation of nutrient deficiency stress by ubiquitination. The plant perceives nutrient deficienc...

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A Program for Iron Economy during Deficiency Targets Specific Fe Proteins

Cited by 72 publications

References 86 publications

The developmental and iron nutritional pattern of PIC1 and NiCo does not support their interdependent and exclusive collaboration in chloroplast iron transport in Brassica napus

The developmental and iron nutritional pattern of PIC1 and NiCo does not support their interdependent and exclusive collaboration in chloroplast iron transport in Brassica napus

Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds

The Role of Selective Protein Degradation in the Regulation of Iron and Sulfur Homeostasis in Plants

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