A programmable culture platform for stimulation and <em>in situ</em> sensing of lung epithelial cells

Abstract

A programmable dynamic cell culture chamber compatible with a standard multi-well plate was designed and characterized. The system is integrated with an array of OECT biosensors, in view of an in-situ monitoring of the dynamic cultures.

Flexible and Stretchable Organic Electrochemical Transistors for Physiological Sensing Devices

“…An emerging technology that holds broad prospects for overcoming the abovementioned limitations is the organic electrochemical transistor (OECT), an ideal multifunction device that is suitable for both biochemical and bioelectrical sensing. Since it was first proposed by Wrighton and co-workers in 1984, [27] OECTs of different types have demonstrated superb abilities in chemical sensing, [28][29][30][31][32][33][34] cell culture and action potential recording, [35][36][37][38][39][40] and health monitoring. [41][42][43][44][45] OECTs exhibit a unique set of advantages for various sensing applications, such as: 1) a biocompatible organic (semi)conductor in the OECT channel making it an ideal platform to interface with biological systems; [46][47][48] 2) fabrication of OECTs is compatible with various low-temperature processing techniques, from low-cost, high-throughput printing methods to conventional photolithography; [49][50][51][52] 3) benefiting from the signal conversion and amplification capabilities of OECTs, high current modulation and fast response can be achieved leading to excellent sensing performance; [53][54][55] 4) operation of OECTs in aqueous electrolyte solutions is suitable for working in complex physiological environments; [56] 5) high compatibility of OECTs with traditional microelectronic integration techniques enables the fabrication of high density sensing arrays for mapping purposes [57][58][59] as well as fabrication of OECT-based microfluidics for multiple analyte detection; [60][61]…”