A prokaryotic phytochrome

Hughes, Jon; Lamparter, Tilman; Mittmann, Franz; Hartmann, E.; Gärtner, Wolfgang; Wilde, Annegret; Börner, Thomas

doi:10.1038/386663a0

Cited by 342 publications

(277 citation statements)

References 12 publications

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“…Some structural plasticity between the GAF and PHY domains as observed for PaBphP and SynCph1 (8, 9, 37) may be involved in long-distance signal transmission. Accordingly, the GAF2 domains of the two copies of SynCph2 (1)(2) in the crystal structure are off-rotated by 6.4°relative to each other, whereas the tongue regions are almost invariantly associated with the GAF1 domains.…”

Section: Resultsmentioning

confidence: 99%

“…The GAF domains of SynCph2 (1)(2) hence mimic the overall organization of canonical photosensory GAF-PHY-modules of phytochromes, including the tongue-like protrusion (Glu 362 -Asn 400 ) that juts of the GAF2 domain and contacts the chromophore-binding pocket of the GAF1 domain (Fig. 1B).…”

Section: Resultsmentioning

confidence: 99%

“…Two models were considered in this work: (a) the crystal structure of SynCph2 (1)(2) itself and (b) a modified structure of SynCph2(1-2) with a planar PCB chromophore as found in the structure of the SynCph1 photosensory module (8).…”

Section: Methodsmentioning

confidence: 99%

“…Orthologous swapping variants of SynCph2 (1)(2) in the tongue and propionate region were produced by adding additional restriction sites into pCDFDuet-1/SynCph2(1-2). Thus the stretches Trp 369 -Glu 391 in the tongue variants and Val 97 -Val 126 in the propionate variants, respectively, were replaced by regions of Cph2-like phytochromes from Nostoc punctiforme PCC 73102 (YP_001868577.1), Oscillatoria sp.…”

Section: Methodsmentioning

confidence: 99%

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Structure of the Cyanobacterial Phytochrome 2 Photosensor Implies a Tryptophan Switch for Phytochrome Signaling

Anders

Daminelli-Widany

Mroginski

et al. 2013

Journal of Biological Chemistry

168

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Background: Phytochromes are red/far-red photoreceptors using a bilin chromophore. Results: Compared with Cph1, the Cph2 bilin-binding site differs around the propionates, but utilizes an otherwise conserved tongue for sealing the chromophore from solvent. Conclusion:The tongue signals via a tryptophan switch within the tongue-GAF domain interface. Significance: The first structure of a Cph2-type phytochrome indicates a common mechanism for photoswitching in all canonical phytochromes.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Structure of the Cyanobacterial Phytochrome 2 Photosensor Implies a Tryptophan Switch for Phytochrome Signaling

Anders

Daminelli-Widany

Mroginski

et al. 2013

Journal of Biological Chemistry

168

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“…In the sequences obtained, a number of ORFs were identified that showed homology to plant-type phytochromes. Subsequent biochemical characterisation has led to the conclusion that the products of these ORFs (e.g., Cph1; see Hughes et al 1997) are members of an entirely new sub-family of the phytochromes, the so-called bacterio-phytochromes (for a review, see Vierstra and Davis 2000). Various subtypes can be distinguished in this family and its members appear to be abundantly present in both phototrophic and chemotrophic bacteria.…”

Section: Involvement Of Bacteriophytochromesmentioning

confidence: 99%

Light-induced behavioral responses (‘phototaxis’) in prokaryotes

Armitage

Hellingwerf

Discoveries in Photosynthesis

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Light-induced sensory responses are among the oldest scientific observations on bacterial behavior. Various types of response have been characterized physiologically in detail. However, the molecular basis of this type of response is only slowly emerging. In many of these systems photosynthetic pigments absorb the light. This then generates a signal via electron transport, feeding into a canonical chemotaxis signal transduction pathway. Nevertheless, several examples have been identified in which dedicated photoreceptor proteins do play a role. The intrinsic complexity of some of these signal transduction systems is overwhelming, in part because of the significant apparent redundancy. The genomics information that is now available for several model organisms (in particular Rhodobacter sphaeroides and Synechocystis sp. PCC6803) facilitates obtaining an increasingly detailed view of the molecular basis of the partial reactions that jointly form the basis of this type of elementary behavioral response.

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Phytochrome Signaling

Quail

2005

Handbook of Photosensory Receptors

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A prokaryotic phytochrome

Cited by 342 publications

References 12 publications

Structure of the Cyanobacterial Phytochrome 2 Photosensor Implies a Tryptophan Switch for Phytochrome Signaling

Structure of the Cyanobacterial Phytochrome 2 Photosensor Implies a Tryptophan Switch for Phytochrome Signaling

Light-induced behavioral responses (‘phototaxis’) in prokaryotes

Phytochrome Signaling

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