1994
DOI: 10.1093/nar/22.3.376
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A proline-rich transcriptional activation domain in murine HOXD-4 (HOX-4.2)

Abstract: ABSTRACT

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Cited by 34 publications
(35 citation statements)
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References 52 publications
(42 reference statements)
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“…Construct A was partially digested with NheI/XbaI, and the resulting full-length Prep2-gfp fusion was introduced into XbaI-digested pRC/ CMV plasmid resulting in construct 1. Gal-O45 is a mammalian expression vector for the GAL4 DBD (30). For construct 3, pPrep2-Topo was digested with EcoRI/BamHI subcloned into BamHI-digested and -blunted Gal-045 3Ј to the GAL-DBD-coding region.…”
Section: Methodsmentioning
confidence: 99%
“…Construct A was partially digested with NheI/XbaI, and the resulting full-length Prep2-gfp fusion was introduced into XbaI-digested pRC/ CMV plasmid resulting in construct 1. Gal-O45 is a mammalian expression vector for the GAL4 DBD (30). For construct 3, pPrep2-Topo was digested with EcoRI/BamHI subcloned into BamHI-digested and -blunted Gal-045 3Ј to the GAL-DBD-coding region.…”
Section: Methodsmentioning
confidence: 99%
“…Some experiments employed 293 T cells constitutively expressing the simian virus 40 large T antigen. Transient transfections were performed using the calcium phosphate precipitation method as described earlier (72). A lacZ reporter driven by the cytomegalovirus (CMV) enhancer was used to control for transfection efficiency in some experiments.…”
Section: Methodsmentioning
confidence: 99%
“…b1-ARE-lacZ consists of the ARE of the Hoxb1 gene (68) cloned by PCR amplification into the HindIII-XhoI sites of p1230. pML, pML(5xHOX-PBX), pML5xHOX, and pML5xUAS are luciferase reporters containing the adenovirus major late promoter alone, driven by 5xHOX-PBX binding sites (TGATTGAT), 5xHOX binding sites (TAAT), or 5xGAL4 binding sites, respectively (67,72,77). Expression plasmids for HOXA1, HOXD4, PBX1A, and PBX1A deletion mutants have been previously described (66,77).…”
Section: Methodsmentioning
confidence: 99%
“…Murine Nanog was divided into three regions based on the position of homeodomain. N-terminal region to the homeodomain is rich in Ser, Thr and Pro residues, a frequent characteristic of transcriptional activation domain (Rambaldi et al, 1994). C-terminal region to the homeodomain contains novel Trp (W) repeat motif of unknown Identification of a putative transactivation dom ain in hum an Nanog structure and function.…”
Section: Introductionmentioning
confidence: 99%