A promiscuity locus confers <i>Lotus burttii</i> nodulation with rhizobia from five different genera

Zarrabian, Mohammad Amin; Montiel, Jesús; Sandal, Niels; Jin, Haojie; Lin, Yu–Chen; Klingl, Verena; Marín, Macarena; James, Euan K.; Parniske, Martin; Stougaard, Jens; Andersen, Stig U.

doi:10.1101/2021.08.26.457880

Cited by 3 publications

(3 citation statements)

References 71 publications

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“…Similarly to S. rostrata , under flooded conditions the robinoid Lotus uliginosus is intercellularly colonized by M. loti , culminating in the formation of nitrogen-fixing nodules ( James and Sprent, 1999 ). Another Lotus species, L. burttii , is an interesting model for studying rhizobial infection, since it can be nodulated by a large diversity of rhizobial species ( Zarrabian et al, 2021 ). The evidence collected indicates that some of these associations occur intercellularly; for instance, the interactions with Sinorhizobium fredii HH103 ( Acosta-Jurado et al, 2016 ) and Rhizobium leguminosarum biovar Norway ( Liang et al, 2019 ), the latter leading to ineffective nodules.…”

Section: Conditional Intercellular Infectionmentioning

confidence: 99%

Molecular Mechanisms of Intercellular Rhizobial Infection: Novel Findings of an Ancient Process

et al. 2022

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Establishment of the root-nodule symbiosis in legumes involves rhizobial infection of nodule primordia in the root cortex that is dependent on rhizobia crossing the root epidermal barrier. Two mechanisms have been described: either through root hair infection threads or through the intercellular passage of bacteria. Among the legume genera investigated, around 75% use root hair entry and around 25% the intercellular entry mode. Root-hair infection thread-mediated infection has been extensively studied in the model legumes Medicago truncatula and Lotus japonicus. In contrast, the molecular circuit recruited during intercellular infection, which is presumably an ancient and simpler pathway, remains poorly known. In recent years, important discoveries have been made to better understand the transcriptome response and the genetic components involved in legumes with obligate (Aeschynomene and Arachis spp.) and conditional (Lotus and Sesbania spp.) intercellular rhizobial infections. This review addresses these novel findings and briefly considers possible future research to shed light on the molecular players that orchestrate intercellular infection in legumes.

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Section: Conditional Intercellular Infectionmentioning

confidence: 99%

Molecular Mechanisms of Intercellular Rhizobial Infection: Novel Findings of an Ancient Process

et al. 2022

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“…Liang et al (2019) described that ineffective R. leguminosarum Norway colonises nodules of L. burttii via cracks in the epidermis. Lotus burttii is more susceptible to less-specific infections (Zarrabian et al, 2021), which is likely to increase its vulnerability to forming an ineffective symbiosis. This reduced specificity by L. burttii is also highlighted in the number of starved plants that contained nodules.…”

Section: Starved L Burttii Plant Nodules Harbour a Microbiome Differe...mentioning

confidence: 99%

Microbiome profiling reveals that Pseudomonas antagonises parasitic nodule colonisation of cheater rhizobia in Lotus

Crosbie¹,

Mahmoudi

Radl

et al. 2022

New Phytologist

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Summary Nodule microbiota are dominated by symbiotic nitrogen‐fixing rhizobia, however, other non‐rhizobial bacteria also colonise this niche. Although many of these bacteria harbour plant‐growth‐promoting functions, it is not clear whether these less abundant nodule colonisers impact root–nodule symbiosis. We assessed the relationship between the nodule microbiome and nodulation as influenced by the soil microbiome, by using a metabarcoding approach to characterise the communities inside nodules of healthy and starved Lotus species. A machine learning algorithm and network analyses were used to identify nodule bacteria of interest, which were re‐inoculated onto plants in controlled conditions to observe their potential functionality. The nodule microbiome of all tested species differed according to inoculum, but only that of Lotus burttii varied with plant health. Amplicon sequence variants representative of Pseudomonas species were the most indicative non‐rhizobial signatures inside healthy L. burttii nodules and negatively correlated with Rhizobium sequences. A representative Pseudomonas isolate co‐colonised nodules infected with a beneficial Mesorhizobium, but not with an ineffective Rhizobium isolate and another even reduced the number of ineffective nodules induced on Lotus japonicus. Our results show that nodule endophytes influence the overall outcome of the root–nodule symbiosis, albeit in a plant host‐specific manner.

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“…In addition, Rhizobial extracellular polysaccharides of various structures, are important factors of nodulation efficiency (Marczak et al, 2017;Maillet et al, 2020). In Lotus a promiscuity locus permitting nodulation of L. burttii with rhizobia from 5 different genera has been located near the LYK cluster but is not due to NFR1 (Zarrabian et al, 2021).…”

Section: The Specificity Of Nodulation Directed By Lyk2bismentioning

confidence: 99%

A newly-evolved chimeric lysin motif receptor-like kinase in Medicago truncatula spp. tricycla R108 extends its Rhizobia symbiotic partnership

Luu

Ourth

Pouzet

et al. 2021

Preprint

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SummaryRhizobial lipochitooligosaccharidic Nod factors, specified by nod genes, are the primary determinants of host specificity in the legume-Rhizobia symbiosis. A Sinorhizobium meliloti nodF/nodL mutant produces Nod factors that differ from wild-type ones in lacking an O-acetate, and with a different acyl chain on the terminal non-reducing sugar. This mutant is defective in nodulation with various Medicago hosts.We examined the nodulation ability of M. truncatula cv Jemalong A17 and M. truncatula ssp. tricycla R108 with the nodF/nodL mutant. We then applied genetic and functional approaches to study the genetic basis and mechanism of nodulation of R108 by this mutant.We show that the nodF/nodL mutant can nodulate R108 but not A17. Using genomics and reverse genetics, we identified a newly-evolved gene in R108, LYK2bis, which is responsible for the phenotype. Transformation with LYK2bis allows A17 to gain nodulation with the nodF/nodL mutant. We found that LYK2bis is involved in specific NF signalling and interacts with the key receptor protein NFP.Our findings reveal that a newly-evolved gene in R108, LYK2bis, extends nodulation specificity to strains producing non-O-acetylated NFs. Interaction between LYK2bis and NFP provides a means of integrating the nodulation signalling pathways.

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A promiscuity locus confers Lotus burttii nodulation with rhizobia from five different genera

Cited by 3 publications

References 71 publications

Molecular Mechanisms of Intercellular Rhizobial Infection: Novel Findings of an Ancient Process

Molecular Mechanisms of Intercellular Rhizobial Infection: Novel Findings of an Ancient Process

Microbiome profiling reveals that Pseudomonas antagonises parasitic nodule colonisation of cheater rhizobia in Lotus

A newly-evolved chimeric lysin motif receptor-like kinase in Medicago truncatula spp. tricycla R108 extends its Rhizobia symbiotic partnership

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