A promoter switch that can rescue a plant sigma factor mutant

Schweer, Jennifer; Loschelder, Heike; Link, Gerhard

doi:10.1016/j.febslet.2006.11.010

Cited by 40 publications

(46 citation statements)

References 36 publications

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“…6B). A similar transcriptional pattern was observed in the SIG6 knockout mutant Schweer et al, 2006) and other PEP-deficient mutants, such as ptac2/6/12 (Pfalz et al, 2006), clb19 (ChateignerBoutin et al, 2008, dg1 (Chi et al, 2008), and ys1 (Zhou et al, 2008). In addition, a 4.8-kb transcript was also detected for rbcL in ptac14-1 (Fig.…”

Section: Discussionsupporting

confidence: 74%

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A Functional Component of the Transcriptionally Active Chromosome Complex, Arabidopsis pTAC14, Interacts with pTAC12/HEMERA and Regulates Plastid Gene Expression

et al. 2011

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The SET domain-containing protein, pTAC14, was previously identified as a component of the transcriptionally active chromosome (TAC) complexes. Here, we investigated the function of pTAC14 in the regulation of plastid-encoded bacterialtype RNA polymerase (PEP) activity and chloroplast development. The knockout of pTAC14 led to the blockage of thylakoid formation in Arabidopsis (Arabidopsis thaliana), and ptac14 was seedling lethal. Sequence and transcriptional analysis showed that pTAC14 encodes a specific protein in plants that is located in the chloroplast associated with the thylakoid and that its expression depends on light. In addition, the transcript levels of all investigated PEP-dependent genes were clearly reduced in the ptac14-1 mutants, while the accumulation of nucleus-encoded phage-type RNA polymerase-dependent transcripts was increased, indicating an important role of pTAC14 in maintaining PEP activity. pTAC14 was found to interact with pTAC12/ HEMERA, another component of TACs that is involved in phytochrome signaling. The data suggest that pTAC14 is essential for proper chloroplast development, most likely by affecting PEP activity and regulating PEP-dependent plastid gene transcription in Arabidopsis together with pTAC12.

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Section: Discussionsupporting

confidence: 74%

“…6A). SIG6 was reported to be essential for PEP to recognize the promoter region of the 2.6-kb transcript Schweer et al, 2006). In ptac14-1, the 2.6-kb transcript cannot be detected (Fig.…”

Section: Discussionmentioning

confidence: 98%

A Functional Component of the Transcriptionally Active Chromosome Complex, Arabidopsis pTAC14, Interacts with pTAC12/HEMERA and Regulates Plastid Gene Expression

et al. 2011

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“…Aberrant Transcription Initiation within the rbcL-accD Intergenic Region in rhon1-2 It was reported that the overaccumulation of some plastid polycistronic transcripts is associated with a change of promoter usage (Haley and Bogorad, 1990;Sexton et al, 1990;Schweer et al, 2006). Overaccumulation of polycistronic rbcL transcripts could be due to the activation of an unknown promoter(s) in rhon1-2.…”

Section: Mutation Of Rhon1 Affects Transcriptional Termination Of Rbclmentioning

confidence: 99%

RHON1 Mediates a Rho-Like Activity for Transcription Termination in Plastids of Arabidopsis thaliana

Chi

Manavski³

et al. 2014

The Plant Cell

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Although transcription termination is essential to generate functional RNAs, its underlying molecular mechanisms are still poorly understood in plastids of vascular plants. Here, we show that the RNA binding protein RHON1 participates in transcriptional termination of rbcL (encoding large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase) in Arabidopsis thaliana. Inactivation of RHON1 leads to enhanced rbcL read-through transcription and to aberrant accD (encoding b-subunit of the acetyl-CoA carboxylase) transcriptional initiation, which may result from inefficient transcription termination of rbcL. RHON1 can bind to the mRNA as well as to single-stranded DNA of rbcL, displays an RNA-dependent ATPase activity, and terminates transcription of rbcL in vitro. These results suggest that RHON1 terminates rbcL transcription using an ATP-driven mechanism similar to that of Rho of Escherichia coli. This RHON1-dependent transcription termination occurs in Arabidopsis but not in rice (Oryza sativa) and appears to reflect a fundamental difference between plastomes of dicotyledonous and monocotyledonous plants. Our results point to the importance and significance of plastid transcription termination and provide insights into its machinery in an evolutionary context.

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“…The activation of a NEP promoter was described in Arabidopsis to compensate for abolished transcription from the atpB PEP promoter (Schweer et al, 2006). We observed that, in the absence of PEP, at least 222 NEP promoters that were undetected by our analysis in green plastids are used in white tissue.…”

Section: Function Of Pep and Nepmentioning

confidence: 50%

The Primary Transcriptome of Barley Chloroplasts: Numerous Noncoding RNAs and the Dominating Role of the Plastid-Encoded RNA Polymerase

et al. 2012

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Gene expression in plastids of higher plants is dependent on two different transcription machineries, a plastid-encoded bacterial-type RNA polymerase (PEP) and a nuclear-encoded phage-type RNA polymerase (NEP), which recognize distinct types of promoters. The division of labor between PEP and NEP during plastid development and in mature chloroplasts is unclear due to a lack of comprehensive information on promoter usage. Here, we present a thorough investigation into the distribution of PEP and NEP promoters within the plastid genome of barley (Hordeum vulgare). Using a novel differential RNA sequencing approach, which discriminates between primary and processed transcripts, we obtained a genome-wide map of transcription start sites in plastids of mature first leaves. PEP-lacking plastids of the albostrians mutant allowed for the unambiguous identification of NEP promoters. We observed that the chloroplast genome contains many more promoters than genes. According to our data, most genes (including genes coding for photosynthesis proteins) have both PEP and NEP promoters. We also detected numerous transcription start sites within operons, indicating transcriptional uncoupling of genes in polycistronic gene clusters. Moreover, we mapped many transcription start sites in intergenic regions and opposite to annotated genes, demonstrating the existence of numerous noncoding RNA candidates.

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A promoter switch that can rescue a plant sigma factor mutant

Cited by 40 publications

References 36 publications

A Functional Component of the Transcriptionally Active Chromosome Complex, Arabidopsis pTAC14, Interacts with pTAC12/HEMERA and Regulates Plastid Gene Expression

A Functional Component of the Transcriptionally Active Chromosome Complex, Arabidopsis pTAC14, Interacts with pTAC12/HEMERA and Regulates Plastid Gene Expression

RHON1 Mediates a Rho-Like Activity for Transcription Termination in Plastids of Arabidopsis thaliana

The Primary Transcriptome of Barley Chloroplasts: Numerous Noncoding RNAs and the Dominating Role of the Plastid-Encoded RNA Polymerase

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