A Proof-of-Concept Clinical Trial of A Single Luteal Use of Long-Acting Gonadotropin-Releasing Hormone Antagonist Degarelix in Controlled Ovarian Stimulation for In Vitro Fertilization: Long Antagonist Protocol

Abstract

IntroductionA drawback of gonadotropin-releasing hormone (GnRH) antagonist protocols in in vitro fertilization (IVF) is that they have limited flexibility in cycle programming. This proof of concept study explored the efficacy of a single-dose, long-acting GnRH antagonist IVF protocol. Trial registration number is NCT03240159, retrospectively registered on March 08, 2017.Materials and methodsThe efficacy of a single-dose long-acting antagonist, degarelix, was explored initially in healthy donors and subsequently in infertile patients. In the first part, five healthy oocyte donors underwent ovarian stimulation with this new protocol: in the late luteal phase, at day 24, a bolus injection of degarelix was administered subcutaneously to control the LH surge in the follicular phase. Ovarian stimulation with gonadotropins was initiated subsequently from day 7 to day 10. End points were first to inhibit the LH surge later in the follicular phase and, second, to retrieve mature oocytes for IVF. In the second part, five infertile women received the same bolus injection of degarelix administered during the luteal phase at day 24. Different gonadotropin starting days (day 2 through day 8) were tested in order to observe possible differences in ovarian stimulation. In these infertile patients, fresh embryo transfers were performed to assess the pregnancy efficacy of this protocol on pregnancy outcomes and to address any possible negative effects on endometrium receptivity.ResultsIn the first part of the study, all donors were effectively downregulated with a single luteal dose of 0.5 ml of degarelix for up to 22 days until the final oocyte maturation triggering day. Mature oocytes were retrieved after 36 h from all patients and all produced 2–7 blastocysts. In the second part, all five infertile patients achieved sufficient LH downregulation and completed ovarian stimulation without any LH surge. All patients (except one with freeze all strategy) had blastocysts transferred and pregnancy occurred in three out of five women.ConclusionA single dose of the long-acting antagonist degarelix during the luteal phase appears to be effective in downregulating hypophysis during ovarian stimulation. This represents a possible new protocol for IVF, which should be further elucidated in RCTs.

Eliminating Hormones With Orally Active Gonadotropin-releasing Hormone Antagonists

“…All 5 patients exhibited effective downregulation of LH levels, and all underwent blastocyst transfer. However, additional larger studies are necessary to reliably assess the effectiveness of this “long-antagonist protocol” compared with standard GnRH-Ant dosing in an ART cycle 69. Younis and colleagues studied the administration of GnRH-Ants on cycle days 1, 2, and 3 for women undergoing a GnRH-Ant cycle.…”

“…However, additional larger studies are necessary to reliably assess the effectiveness of this "long-antagonist protocol" compared with standard GnRH-Ant dosing in an ART cycle. 69 Younis and colleagues studied the administration of GnRH-Ants on cycle days 1, 2, and 3 for women undergoing a GnRH-Ant cycle. While they observed a longer stimulation period for the study groups versus controls (10.9 ± 3.1 vs. 9.7 ± 1.3 d, respectively), the number of follicles > 14 mm and estradiol was similar between the 2 groups.…”