A proposal for standardization in forensic bovine DNA typing: allele nomenclature of 16 cattle‐specific short tandem repeat loci

Abstract

In this study, a proposal is presented for the allele nomenclature of 16 polymorphic short tandem repeat (STR) loci (BM1824, BM2113, ETH10, ETH225, INRA023, SPS115, TGLA122, TGLA126, TGLA227, ETH3, TGLA53, BM1818, CSRM60, CSSM66, HAUT27 and ILSTS006) for bovine genotyping (Bos taurus). The nomenclature is based on sequence data of the polymorphic region(s) of the STR loci as recommended by the DNA commission of the International Society of Forensic Genetics for human DNA typing. To cover commonly and rarely occurring alleles, a selection of animals homozygous for the alleles at these STR loci were analysed and subjected to sequence studies. The alleles of the STR loci consisted either of simple or compound dinucleotide repeat patterns. Only a limited number of alleles with the same fragment size showed different repeat structures. The allele designation described here was based on the number of repeats including all variable regions within the amplified fragment. The set of 16 STR markers should be propagated for the use in all bovine applications including forensic analysis.

Population studies and parentage testing for Arabian horses using 15 microsatellite markers

“…The sexlinked LEX3 locus included in the Equine STOCKMARKS Ò was not used in any statistical analysis. Parentage verification, PI and POP were tested using DNASTAT v.1.1 software.Allele frequencies and statistical results: The number of alleles varied from 5 to 10, with the average number of alleles being 7.56 for this study (Table 1), which is a narrower range than found in another recent study (Van de Goor et al 2009). The observed heterozygosity and expected heterozygosity ranged from 0.399 to 0.746.…”

Development of the first standardised panel of two new microsatellite multiplex PCRs for gilthead seabream (Sparus aurata L.)

“…The human forensic community has chosen larger repeat motif microsatellite markers, as they have a lower probability of error (Perlin et al 1995;Gagneux et al 1997;Van de Goor et al 2009a). However, genetic studies on fishes have traditionally used dinucleotide markers, as just a few tri-or tetranucleotides have been isolated and, in general, they show a lower variability (Van de Goor et al 2009b).…”

Short communication: Parentage verification of South African Angora goats, using microsatellite markers

“…A large number of microsatellite markers are available for most farm animal species and parentage panels have been commercialized and used in the routine testing of dairy and beef cattle (Van Eenennaam et al, 2007;Van de Goor et al, 2009). The goat genome has not been studied as widely when compared to other farm animal species (Fadiel et al, 2005;Maddox & Cockett, 2007) These markers were tested in several laboratories and results generally compared well with regard to individual marker performance.…”