A proposed classification of screening methods for plant breeding programs

Britten, E. J.

doi:10.1007/bf00029481

Euphytica

1960

DOI: 10.1007/bf00029481

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A proposed classification of screening methods for plant breeding programs

E. J. Britten¹

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2024

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The Application of Fluorescence In Situ Hybridization in the Prescreening of Veronica Hybrids

Park,

Sevilleno,

et al. 2024

Plants

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Fluorescence in situ hybridization (FISH), a molecular cytogenetic technique that enables the visualization and identification of specific DNA sequences within chromosomes, has emerged as a pivotal tool in plant breeding programs, particularly in the case of Veronica species. Veronica, a genus with a complex reproductive system, often poses challenges in accurately identifying hybrids because of its tendency to hybridize, which leads to intricate genetic variation. This study focused on the use of FISH as a prescreening method to identify true hybrids in Veronica breeding programs. FISH analysis was first performed on the parents to identify their 45S and 5S rDNA signals, along with their respective chromosome numbers. The signals were then compared with those of the twenty progenies with reference to their supposed parents. Five true hybrids, seven self-pollinated progenies, and eight false hybrids were identified through FISH. The findings highlight the significance of FISH as a screening method that contributes significantly to the efficiency of Veronica breeding programs by ensuring the preservation of desired genetic traits and minimizing the inadvertent inclusion of misidentified hybrids. To conclude, this study underscores the vital role of FISH in enhancing the precision and success of breeding programs and opens new avenues for improved breeding strategies and crop development.

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The Application of Fluorescence In Situ Hybridization in the Prescreening of Veronica Hybrids

Park,

Sevilleno,

et al. 2024

Plants

View full text Add to dashboard Cite

show abstract

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A proposed classification of screening methods for plant breeding programs

Cited by 1 publication

References 13 publications

The Application of Fluorescence In Situ Hybridization in the Prescreening of Veronica Hybrids

The Application of Fluorescence In Situ Hybridization in the Prescreening of Veronica Hybrids

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