This paper aims to analyze a measurement tool allowing the identification of the differences that may exist between the factors and their relationships that affect the entrepreneurial intention of undergraduate students from related and non-business-related programs. This is to know what elements motivate to create a company for each group, and their degree of association, in order to design more effective educational strategies focused on their motivations. For this, a model was proposed, which was validated through the application of a self-administered questionnaire to undergraduate students of different careers, in Instituto Tecnológico Metropolitano (Medellín). It was found that personal attitudes have a stronger relationship with entrepreneurial intention in students of business-related programs, while perceived viability, perceived convenience, and entrepreneurial behavior have greater weight in students from non-affiliated programs. Thus, the courses focused on creativity in the first group, and the courses focused on the know-how in the second group would be more efficient in increasing the entrepreneurial intention.