A proposed nomenclature of the domestic cat karyotype

Cho, K W; Youn, Hwa Young; Watari, Toshihiro; Tsujimoto, Hajime; Hasegawa, Akihiro; Satoh, Hitoshi

doi:10.1159/000134686

Cited by 35 publications

(22 citation statements)

References 11 publications

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“…Together, these markers cover all 18 feline autosomes and X and Y chromosomes, with all but two loci occupying unique coordinates on the map. Twohundred ninety-one loci have been ordered at odds Ն1000:1, with an additional 54 loci ordered at odds (Cho et al 1997d) are shown to the left of each map. Hybridization results from human on cat chromosome painting (Rettenberger et al 1995;Wienberg et al 1997) are depicted to the left of each ideogram for comparison to the RH-derived syntenies.…”

Section: Map Construction and Characterizationmentioning

confidence: 99%

A Radiation Hybrid Map of the Cat Genome: Implications for Comparative Mapping

Murphy¹,

Sun²,

Chen³

et al. 2000

Genome Res.

121

100

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Ordered gene maps of mammalian species are becoming increasingly valued in assigning gene variants to function in human and animal models, as well as recapitulating the natural history of genome organization. To extend this power to the domestic cat, a radiation hybrid (RH) map of the cat was constructed integrating 424 Type I-coding genes with 176 microsatellite markers, providing coverage over all 20 feline chromosomes. Alignment of parallel RH maps of human and cat reveal 100 conserved segments ordered (CSOs) between the species, nearly three times the number observed with reciprocal chromosome painting analyses. The observed number is equivalent to theoretical predictions of the number of conserved segments to be found between cat and human, implying that 300-400 Type I gene markers is sufficient to reveal nearly all conserved segments for species that exhibit the most frequently observed "slow" rate of genome reorganization. The cat-human RH map comparisons provide a new genomic tool for comparative gene mapping in the cat and related Felidae, and provide confirmation that the cat genome organization is remarkably conserved compared with human. These data demonstrate that ordered RH-based gene maps provide the most precise assessment of comparing genomes, short of contig construction or full-sequence determination.

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Section: Map Construction and Characterizationmentioning

confidence: 99%

A Radiation Hybrid Map of the Cat Genome: Implications for Comparative Mapping

Murphy¹,

Sun²,

Chen³

et al. 2000

Genome Res.

121

100

View full text Add to dashboard Cite

show abstract

“…The probe was purified using a G50 Sephadex column (Pharmacia) following the manufacturer's instructions and Cho et al (1997b).…”

Section: Mapping Of Type I Markers Onto Dog Chromosomesmentioning

confidence: 99%

“…1b. The application of the CFA 5 paint to cat metaphase chromosome spreads revealed blocks of signal on four chromosome pairs. These regions were identified as FCA C1p22, D1p13→ p12, E1p14 → p13, and E2q13 → q14 on the basis of their DAPI-banding patterns, as compared to the G-banded ideogram of Cho et al (1997b). The data are presented in Fig.…”

Section: Reciprocal Chromosome Paintingmentioning

confidence: 99%

Zoo-FISH analysis of dog chromosome 5: identification of conserved synteny with human and cat chromosomes

Thomas

Breen

Langford

et al. 1999

Cytogenet Genome Res

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Conserved segments of synteny between the human genome and chromosome 5 (CFA 5) of the domestic dog (Canis familiaris) have been identified by reciprocal chromosome painting analysis. A CFA 5 paint probe was applied to human metaphase spreads, revealing distinct hybridisation sites on human (HSA) chromosomes 1, 11, 16, and 17. Paint probes for these human chromosomes were then hybridised to dog metaphase spreads, identifying the regions of CFA 5 with which homology is shared with the corresponding human chromosome. Application of the CFA 5 paint probe to metaphase spreads of the domestic cat (Felis catus, FCA) demonstrated hybridisation to cat chromosomes C1, D1, E1, and E2. Dog PCR primers for type 1 markers known to lie in the corresponding regions of HSA 11, 16, and 17 were used to isolate dog BAC clones representing four genes. Fluorescence in situ hybridisation analysis confirmed their localisation to CFA 5 and suggested that two of the conserved segments lie in opposing orientations on CFA 5, compared to the human chromosome concerned. A third segment appears to lie in the same orientation on both human and dog chromosomes. No suitable gene markers were available for analysis of the fourth segment. The significance of these findings is discussed with reference to current and future dog genome mapping efforts.

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“…The FGFasL clone contained F11-kb insert of feline genomic FasL and was used for FISH. Based on the nomenclature for feline Q-banded chromosomes (Cho et al, 1997), the chromosome locus was determined. (Mizuno et al, 1998) Gene reference: The nucleotide sequence of feline FasL cDNA was published in Mizuno et al (1998).…”

Section: Methodsmentioning

confidence: 99%