A proposed role for diffusible signal factors in the biofilm formation and morphological transformation of Helicobacter pylori

Krzyżek, Paweł; Gościniak, Grażyna

doi:10.5152/tjg.2017.17349

Cited by 28 publications

(22 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At the cellular level, the best-described morphological change in Hp associated with biofilm in vivo is coccoid transformation (Carron et al, 2006; Coticchia et al, 2006; Cellini et al, 2008). Coccoid transformation is considered a common feature of Gram-negative rod bacteria under conditions of stress (Andersen and Rasmussen, 2009; van Teeseling et al, 2017; Krzyzek and Gosciniak, 2018). In vitro , coccoid forms of Hp can be induced by prolonged culture or use of suboptimal levels of antibiotics (Krzyzek and Gosciniak, 2018; Poursina et al, 2018).…”

Section: Functional and Morphological Changes Associated With Biofilmmentioning

confidence: 99%

“…Coccoid transformation is considered a common feature of Gram-negative rod bacteria under conditions of stress (Andersen and Rasmussen, 2009; van Teeseling et al, 2017; Krzyzek and Gosciniak, 2018). In vitro , coccoid forms of Hp can be induced by prolonged culture or use of suboptimal levels of antibiotics (Krzyzek and Gosciniak, 2018; Poursina et al, 2018). The Hp coccoid forms have been defined as viable but non-culturable (VBNC), thus undetectable by conventional cultures, and are known to have an increased capacity to aggregate into monomicrobial clusters embedded in thick EPS (Cellini et al, 2008; Cellini, 2014; Krzyzek and Gosciniak, 2018).…”

Section: Functional and Morphological Changes Associated With Biofilmmentioning

confidence: 99%

See 1 more Smart Citation

Potential Role of Biofilm Formation in the Development of Digestive Tract Cancer With Special Reference to Helicobacter pylori Infection

et al. 2019

View full text Add to dashboard Cite

Bacteria are highly social organisms that communicate via signaling molecules and can assume a multicellular lifestyle to build biofilm communities. Until recently, complications from biofilm-associated infection have been primarily ascribed to increased bacterial resistance to antibiotics and host immune evasion, leading to persistent infection. In this theory and hypothesis article we present a relatively new argument that biofilm formation has potential etiological role in the development of digestive tract cancer. First, we summarize recent new findings suggesting the potential link between bacterial biofilm and various types of cancer to build the foundation of our hypothesis. To date, evidence has been particularly convincing for colorectal cancer and its precursor, i.e., polyps, pointing to several key individual bacterial species, such as Bacteroides fragilis, Fusobacterium nucleatum , and Streptococcus gallolyticus subsp. Gallolyticus. Then, we further extend this hypothesis to one of the most common bacterial infection in humans , Helicobacter pylori ( Hp ), which is considered a major cause of gastric cancer. Thus far, there has been no direct evidence linking in vivo Hp gastric biofilm formation to gastric carcinogenesis. Yet, we synthesize the information to support an argument that biofilm associated- Hp is potentially more carcinogenic, summarizing biological characteristics of biofilm-associated bacteria. We also discuss mechanistic pathways as to how Hp or other biofilm-associated bacteria control biofilm formation and highlight recent findings on Hp genes that influence biofilm formation, which may lead to strain variability in biofilm formation. This knowledge may open a possibility of developing targeted intervention. We conclude, however, that this field is still in its infancy. To test the hypothesis rigorously and to link it ultimately to gastric pathologies (e.g., premalignant lesions and cancer), studies are needed to learn more about Hp biofilms, such as compositions and biological properties of extracellular polymeric substance (EPS), presence of non- Hp microbiome and geographical distribution of biofilms in relation to gastric gland types and structures. Identification of specific Hp strains with enhanced biofilm formation would be helpful not only for screening patients at high risk for sequelae from Hp infection, but also for development of new antibiotics to avoid resistance, regardless of its association with gastric cancer.

show abstract

Section: Functional and Morphological Changes Associated With Biofilmmentioning

confidence: 99%

Section: Functional and Morphological Changes Associated With Biofilmmentioning

confidence: 99%

Potential Role of Biofilm Formation in the Development of Digestive Tract Cancer With Special Reference to Helicobacter pylori Infection

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Morphological plasticity as a mechanism enabling the adaptation of microbes to changing environmental conditions may explain the high heterogeneity of the H. pylori population [ 13 ] ( Figure 1 ). Classical classification of H. pylori morphological forms includes the division into: live, culturable spiral forms – related to the process of host colonisation, and live, non-culturable coccoid forms – associated with the viable but non-culturable (VBNC) process and the survival of this bacterium under unfavourable conditions [ 14 ]. In addition, the existence of rod-shaped and filamentous forms of H. pylori is also noted [ 7 , 8 , 15 , 16 ].…”

Section: Morphological Forms Of Helicobacter Pylorimentioning

confidence: 99%

Morphology of Helicobacter pylori as a result of peptidoglycan and cytoskeleton rearrangements

Krzyżek¹,

Gościniak²

2018

View full text Add to dashboard Cite

Helicobacter pylori is a Gram-negative, microaerophilic bacterium colonising the gastric mucosa. Normally, this bacterium has a spiral shape, which is crucial for proper colonisation of the stomach and cork-screwing penetration of dense mucin covering this organ. However, H. pylori may also form curved/straight rods, filamentous forms and coccoid forms. This morphological variability affects nutrient transport and respiration processes, as well as motility, the ability to form aggregates/biofilms, and resistance to adverse environmental factors. For this reason, a more accurate understanding of the molecular determinants that control the morphology of H. pylori seems to be crucial in increasing the effectiveness of antibacterial therapies directed against this microorganism. This article focuses on the molecular factors responsible for peptidoglycan and cytoskeleton rearrangements affecting H. pylori morphology and survivability. In addition, the existence of proteins associated with modifications of H. pylori morphology as potential targets in therapies reducing the virulence of this bacterium has been suggested.

show abstract

“…9,23 Streptococci are a source of SDSF (Streptococcus diffusible signal factors), which may be involved in the morphological transformation of H. pylori into coccoid forms. 48 In an experiment, Khosravi et al determined the effect of H. pylori and S. mitis co-culture on the gene expression of both bacteria. 47 It was observed that streptococci expressed genes encoding phosphoglycerate kinase (PGK) only when co-existing with H. pylori.…”

Section: Interactions Of Oral H Pylori With Microbial Fl Oramentioning

confidence: 99%

Oral Helicobacter pylori: Interactions with host and microbial flora of the oral cavity

Krzyżek

Gościniak

2018

Dent. Med. Probl.

View full text Add to dashboard Cite

The role of the oral cavity as a reservoir for Helicobacter pylori is still a controversial issue. There is a great number of articles indicating the presence of this pathogen in the oral cavity, but discrepancies among techniques for H. pylori detection and the variations in the patients tested often make it difficult to formulate a final verdict. Outer membrane proteins (OMPs) are one of the most important factors determining colonization of H. pylori in the oral cavity. Among them, the key role is attributed to BabA, SabA and NapA, all of which promote adherence and retention within this area. The oral cavity is characterized by the co-existence of numerous microorganisms which may potentially affect the physiology and morphology of H. pylori. The presence of coccoid-stimulating factors and relatively low levels of AI-2 in the early-to mid-stages of supragingival plaque allow dental H. pylori to colonize this niche as nonculturable spherical forms. On the other hand, subgingival plaque characterized by high numbers of periopathogens, capable of synthesizing high concentrations of AI-2, may favor the presence of mixed populations of spiral and coccoid H. pylori forms. This review article provides an up-to-date knowledge about the ability of oral H. pylori to interact both with the host and the local microflora of the oral cavity.

show abstract

A proposed role for diffusible signal factors in the biofilm formation and morphological transformation of Helicobacter pylori

Abstract: Abstract

Cited by 28 publications

References 47 publications

Potential Role of Biofilm Formation in the Development of Digestive Tract Cancer With Special Reference to Helicobacter pylori Infection

Potential Role of Biofilm Formation in the Development of Digestive Tract Cancer With Special Reference to Helicobacter pylori Infection

Morphology of Helicobacter pylori as a result of peptidoglycan and cytoskeleton rearrangements

Oral Helicobacter pylori: Interactions with host and microbial flora of the oral cavity

Contact Info

Product

Resources

About