2010
DOI: 10.1073/pnas.1001323107
|View full text |Cite
|
Sign up to set email alerts
|

A prospective analysis of the Ab response to Plasmodium falciparum before and after a malaria season by protein microarray

Abstract: Abs are central to malaria immunity, which is only acquired after years of exposure to Plasmodium falciparum (Pf). Despite the enormous worldwide burden of malaria, the targets of protective Abs and the basis of their inefficient acquisition are unknown. Addressing these knowledge gaps could accelerate malaria vaccine development. To this end, we developed a protein microarray containing ∼23% of the Pf 5,400-protein proteome and used this array to probe plasma from 220 individuals between the ages of 2-10 year… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

28
483
4
1

Year Published

2011
2011
2019
2019

Publication Types

Select...
5
4

Relationship

1
8

Authors

Journals

citations
Cited by 401 publications
(516 citation statements)
references
References 49 publications
28
483
4
1
Order By: Relevance
“…This is consistent with other studies that failed to identify "protective" antibodies (37,38). And, protein arrays probed by samples from Malian individuals at the end of the transmission season did not associate with risk, whereas samples taken before the transmission season did (18). Among the 22 differentially reactive antibody responses found in this comparison, most antibody targets were asexual blood-stage antigens, and eight were PfEMP1 proteins, which are major proteins that are clonally variant antigens expressed on the surface of infected red blood cells and targets of protective antibodies (39).…”
Section: Discussionsupporting
confidence: 80%
See 1 more Smart Citation
“…This is consistent with other studies that failed to identify "protective" antibodies (37,38). And, protein arrays probed by samples from Malian individuals at the end of the transmission season did not associate with risk, whereas samples taken before the transmission season did (18). Among the 22 differentially reactive antibody responses found in this comparison, most antibody targets were asexual blood-stage antigens, and eight were PfEMP1 proteins, which are major proteins that are clonally variant antigens expressed on the surface of infected red blood cells and targets of protective antibodies (39).…”
Section: Discussionsupporting
confidence: 80%
“…Each pad contains 824 peptide fragments expressed from Pf ORFs representing 702 unique proteins and three concentrations of 14 full-length proteins expressed using clinicalgrade manufacturing procedures. The protein targets on the array were selected from published studies done on a larger microarray containing 2,323 protein features after interrogating specimens from naturally exposed individuals (18), and specimens from sporozoite vaccine trials (19). The top 824 most immunoreactive antigens from these studies were printed on this down-selected array.…”
Section: Figmentioning
confidence: 99%
“…43,44 Additionally, anti-CS antibodies appear to be associated with protection against natural exposure of vaccinated 45,46 and non-vaccinated human volunteers. 47,48 Although a recent study indicates that there appears to be no association between levels of anti-CS antibodies and protection, 49 a possible explanation for these discrepancies may be the different antigens used for antibody detection. The CS antigen is a large protein with numerous B-cell epitopes distributed along its entire sequence and therefore while antibodies to certain regions might not be associated with protection, antibodies to epitopes comprising or related to functional domains, i.e., those comprising region I within the N region may block the protein cleavage necessary for parasite invasion and are likely to be associated with protection.…”
Section: Discussionmentioning
confidence: 97%
“…For instance, in a Tanzanian study where altitude was a surrogate for exposure level, it was observed that levels of antibodies to merozoite surface protein (MSP)‐1 19 , MSP‐2 and AMA‐1 declined with increasing altitude (lower exposure levels) 101. Similarly, higher seroprevalence and higher antibody titres were observed in children and adults immediately after the high‐transmission season (a period of intense exposure) relative to observed levels before the malaria season (a period of little to no transmission) 99. Two Senegalese studies also recently reported a decline in seroprevalence of antibodies to both pre‐erythrocytic and erythrocytic stage antigens in children, as the entomological inoculation rate (EIR) declined over the years 102, 103.…”
Section: The Role Of Inflammation In Malarial Pathogenesismentioning
confidence: 99%