The aim of this study is to determine the reliability and validity of the Adapted Turkish “Students’ Attitudes Towards Addressing Sexual Health” questionnaire (SA-SH-TR). This methodological study was conducted in two stages. In the first stage, the language was adapted to Turkish. Second, the reliability of the scale was evaluated. From January 2018 to March 2018, 292 nursing students volunteered to participate in the study. The content validity index of the Turkish version of the questionnaire ranged from 0.76 to 1.00 and was found to be high. Cronbach’s alpha coefficient was 0.89. Removal of Items 11, 12, 14, 16, 17, 18, 20, and 22 would have improved alpha to 0.91. It was observed that the item-total correlation values of the items changed between 0.24 and 0.75. Test–retest interclass correlation coefficient was 0.83. The study findings determined that the Turkish adaptation of the SA-SH-TR was valid and reliable and could be used in nursing research and practice.