A prospective randomized study comparing aspiration only with aspiration and flushing for transvaginal ultrasound-directed oocyte recovery

Tan, Seang‐Lin; Waterstone, John; Wren, Marie; Parsons, John

doi:10.1016/s0015-0282(16)55230-3

Cited by 64 publications

(45 citation statements)

References 8 publications

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“…No beneficial effect of follicle flushing during US-guided OPU has been reported using double-lumen needles [12,18] when in vivo matured oocytes were retrieved from human preovulatory follicles, where the junction between the cumulus cells and follicular wall is loose. Bovine oocytes are usually aspirated from small antral follicles in USguided OPU.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Twisting and Type of Aspiration Needle on the Efficiency of Transvaginal Ultrasound-Guided Ovum Pick-Up in Cattle

Sasamoto

Sakaguchi

Katagiri

et al. 2003

The Journal of Veterinary Medical Science

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The effects of twisting and type (single-or double-lumen) of aspiration needle on the efficiency of transvaginal ultrasound-guided ovum pickup (US-guided OPU) were investigated in cattle. The first study using slaughterhouse ovaries revealed that twisting of the needle during follicle aspiration improved the oocyte recovery rate without deleterious effects on the attachment of cumulus layers. Vacuum pressure affected the oocyte recovery and cumulus attachment, regardless of the needle type. The needle type did not affect the oocyte recovery or cumulus attachment with an optimized vacuum pressure. In the second study, US-guided OPU was performed in live cows using two types of needles with a vacuum pressure of 75 mmHg. The needle type did not affect the oocyte recovery or cumulus attachment of the recovered oocytes. The results revealed that twisting of the needle is effective in follicle aspirati on, and suggested that a single-lumen needle is as useful as a double-lumen needle for US-guided OPU in cattle. The transvaginal ultrasound-guided ovum pickup (US-guided OPU) in cattle reported by Pieterse et al. [16] has been widely utilized as a new technique which enables repeated retrieval of oocytes from live cows in various conditions , including milking [5], dry [5], pregnant [15], prepu-bertal [4, 8] and infertile cows [10]. Several factors, such as hormonal stimulation [8, 14], time of US-guided OPU within the estrous cycle [17] and frequency within a certain period [9], have been reported to affect the efficiency of recovery of oocytes and quality of cumulus-oocyte-complexes (COCs). However, even if many follicles can be recruited in vivo by these modifications, a reliable follicle aspiration system is required. Nevertheless, there are few reports of attempts to improve the follicle aspiration system. Recently, follicle aspiration experiments using slaughterhouse ovaries showed that vacuum pressure [2], and the diameter [2, 7] and bevel [3] of aspiration needles affected the oocyte recovery rate, cumulus attachment and quality of the oocytes. A double-lumen needle allows simultaneous flushing and aspiration of follicles, overcoming the potential problem of failure to retrieve oocytes by only a single aspiration. However, few reports [6] have demonstrated the superiority of double-lumen needles to single-lumen needles in live cows. Although follicle aspiration with needle twisting has been carried out to retrieve follicular oocytes from live cows [16, 19], the effects of needle twisting on the oocyte recovery rate and the quality of recovered oocytes have not been determined. The aim of this study was to investigate the effects of twisting and type (single-or double-lumen) of aspiration needle on the efficiency of oocyte retrieval using slaughterhouse ovaries and live cows. MATERIALS AND METHODS Follicle aspiration system: A single-lumen needle (18 gauge, 60-cm long; Fujihira Industries, Tokyo, Japan) was connected to a 50-ml plastic conical tube (Falcon 2098;

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Section: Discussionmentioning

confidence: 99%

The Effects of Twisting and Type of Aspiration Needle on the Efficiency of Transvaginal Ultrasound-Guided Ovum Pick-Up in Cattle

Sasamoto

Sakaguchi

Katagiri

et al. 2003

The Journal of Veterinary Medical Science

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“…Nevertheless, with the development of oocyte retrieval technology, an increasing number of studies have found that skilled oocyte retrieval by direct aspiration can be used to obtain the vast majority of oocytes. Conversely, the use of a DLN for follicular flushing would prolong the operative time, affect the fertilization of oocytes, and confer no benefit compared with the use of a single-lumen needle (SLN) [6] [7]. A meta-analysis published by Roque et al in 2012 concluded that in an unselected group of patients, there was no advantage in using a DLN in oocyte retrieval [8].…”

mentioning

confidence: 99%

Double-Lumen Needle Follicular Flushing System versus Single-Lumen Aspiration Needle in IVF/ICSI Patients with Poor Ovarian Response: A Meta-Analysis

Xing¹,

Ou²,

Cai³

et al. 2017

ASM

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Background: The present study performed a meta-analysis to comprehensively analyze existing randomized controlled trials (RCT) involving the use of double-lumen needle in patients with poor ovarian response to explore whether double-lumen needle was good for specific patients. Methods: The PubMed, EMBASE, Cochrane Library databases and two randomized controlled trials registration centers were thoroughly searched until April 2017. The clinical outcomes of IVF/ICSI cycles were compared between two groups with double-lumen needle and single-lumen needle. Results: Four RCT studies were included in this present meta-analysis. The oocytes yield was similar in two groups (OR 0.88,; I 2 = 4%). The procedure time with

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“…There was no difference in the number of germinal vesicles (immature oocytes) between the groups which we may expect to see with an increase total number of oocytes retrieved. No other oocyte retrieval techniques, including follicle flushing and altering needle characteristics have been associated with increased oocyte yield [2][3][4][5][6][7][8]. This benefit would appear to outweigh the three extra minutes required to implement curetting during the retrieval procedure.…”

Section: Discussionmentioning

confidence: 99%

“…Follicle flushing after aspiration of the follicular fluid has not been shown to increase the number of oocytes retrieved by most studies [2][3][4][5]. The type and caliber of the needle used during oocyte retrieval was also examined and, while pain was directly associated with needle caliber, there were no significant differences in pregnancy outcomes [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Follicle curetting at the time of oocyte retrieval increases the oocyte yield

Dahl

Cannon

Aubuchon

et al. 2009

J Assist Reprod Genet

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Purpose To determine whether follicle curetting at the time of oocyte retrieval increases oocyte yield. Methods Retrospective review of all patients who underwent oocyte retrieval from July 1, 2003 to June 30, 2005. Main outcome measure Number of oocytes retrieved. Secondary outcome measures: retrieval time, number of cryopreserved embryos, pregnancy rates, and incidence of ovarian hyperstimulation syndrome. Results There were no differences in patient demographics, antral follicle count, cycle stimulation characteristics, fertilization rates, embryo quantity or quality, embryo cryopreservation rates, clinical pregnancy rates, live birth rates, or ovarian hyperstimulation syndrome between the groups. Retrievals that utilized curetting took three minutes longer. Follicle curetting significantly increased the number of oocytes retrieved, 13.9±0.6 compared to 11.4±0.6 oocytes without curetting (P=0.003). The quantity of mature oocytes was also increased with curetting (10.3± 0.5 versus 8. 4±0.5, P=0.006). Conclusions This study demonstrated that follicle curetting significantly increased oocyte yield. While it did not increase live birth rates, this increase in oocyte yield should lead to increased numbers of embryos for selection at transfer and increased embryos for cryopreservation.

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A prospective randomized study comparing aspiration only with aspiration and flushing for transvaginal ultrasound-directed oocyte recovery

Cited by 64 publications

References 8 publications

The Effects of Twisting and Type of Aspiration Needle on the Efficiency of Transvaginal Ultrasound-Guided Ovum Pick-Up in Cattle

The Effects of Twisting and Type of Aspiration Needle on the Efficiency of Transvaginal Ultrasound-Guided Ovum Pick-Up in Cattle

Double-Lumen Needle Follicular Flushing System versus Single-Lumen Aspiration Needle in IVF/ICSI Patients with Poor Ovarian Response: A Meta-Analysis

Follicle curetting at the time of oocyte retrieval increases the oocyte yield

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