The main objective was to study the prescribing pattern of antibiotic in a tertiary care hospital to assess the frequency and prescribing the practice of antibiotics. The study includes an observational study of antibiotic prescribing patterns conducted over 6 months. In this study, enrolled 300 patients who were prescribed with antibiotics from both outpatient and inpatient department. Data were collected from the patient's chart and was subjected to analyze by performing descriptive statistics. In the present study, the prescription was assessed and found the maximum number of patients belonged to the age group of 21-30 years, and the proportion of male patients was more compared to the female patients. Moreover, younger and adult patients were commonly associated with the highest prescribed antibiotics in both male and female patients. Also observed that the frequency of antibiotics were 1, 2, and 3 or > 3 prescribed to 55%, 26%, and 19% respectively. Among the all group of antibiotics cephalosporins was found to be prescribed to the largest number (22.03%) of patients, followed by Quinolone (15.57%), Antifungals (14.36%), Aminoglycosides (11.59%), Penicillin (10.76%), Antiamoebic (8.29%), Macrolides (7.31%), Antimalarial (6.44%) and others were (3.65%) Among the antibiotics cefixime, ciprofloxacin, Itraconazole, Amoxicillin, Amikacin, and Azithromycin most chosen drugs % of antibiotic prescribed to male were much higher than female patient's especially penicillins, quinolones and cephalosporins group of antibiotics whereas antifungal and macrolides group of antibiotics higher in female patients than male patients.