A protein encoded by circular ZNF609 RNA induces acute kidney injury by activating the AKT/mTOR-autophagy pathway

Ouyang, Xin; Zhao, He; Fang, Heng; Zhang, Huidan; Yin, Qinye; Hu, Linhui; Gao, Fei; Yin, Hao; Hao, Taofang; Hou, Yuguang; Wu, Qingping; Jia, Dechang; Xu, Jing; Wang, Yirong; Chen, Chunbo

doi:10.1016/j.ymthe.2022.09.021

Cited by 9 publications

(3 citation statements)

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“…[53][54][55] There are multiple other documented examples of circRNAs that affect the regulation of the immune system and the progression of cancer and diseases. [56][57][58][59][60][61] CircRNA role in neurodegenerative diseases. CircRNAs have been implicated in the development of neurodegenerative diseases.…”

Section: Regulation Of Circrna Biosynthesismentioning

confidence: 99%

Circular RNA in Multiple Sclerosis: Pathogenicity and Potential Biomarker Development: A Systematic Review

Mohammed

2023

Genet Epigenet

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Multiple sclerosis (MS) is a complex autoimmune disorder of the CNS that affects millions of people worldwide. The causes of the disease remain unknown despite extensive efforts to understand it. CircRNAs are a unique class of endogenous non-coding RNA that are abundant, stable, conserved, and specifically expressed molecules, making them a promising biomarker of diseases. This review investigates the role of circRNA in MS pathogenicity and their potential as a biomarker through a comprehensive literature search conducted in 8 scientific databases. The studies found that there are differentially expressed circRNAs in MS patients compared to healthy controls (HC), and this difference is even more pronounced in different MS subtypes. Enrichment of circRNAs in linkage disequilibrium (LD) blocks that harbor MS-associated SNPs suggests that these SNPs manipulate the levels of circRNAs in the surrounding area, contributing to disease pathogenicity. While circRNA shows promise as an indicator or biomarker for MS disease pathology, further research is needed to fully explore its potential and impact on human biology.

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Section: Regulation Of Circrna Biosynthesismentioning

confidence: 99%

Circular RNA in Multiple Sclerosis: Pathogenicity and Potential Biomarker Development: A Systematic Review

Mohammed

2023

Genet Epigenet

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“…Multiple pathological factors and characteristic mechanisms may lead to the occurrence of CSA-AKI, including cardiac circulation disorders, renal embolism, renal side effects of cardiopulmonary bypass, as reflected in renal ischaemia-reperfusion (IR), haemolysis, inflammation and oxidative stress. [7][8][9] Recent studies showed that changes in intestinal microbiota composition were accompanied by ischaemic AKI in animal models. 10 The gut microbiota plays an important role in the immune system, intestinal endocrine function and the production of numerous compounds influencing the host.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic interplay between gut microbiome and plasma metabolome in cardiac surgery‐associated acute kidney injury

Bai¹,

Huang²,

Jiang

et al. 2023

Rapid Comm Mass Spectrometry

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Rationale Cardiac surgery‐associated acute kidney injury (CSA‐AKI) is a prevalent complication of cardiac surgery, which may be associated with a great risk of developing chronic kidney disease and mortality. This study aimed to investigate the possible links between gut microbiota metabolism and CSA‐AKI. Methods A prospective cohort of patients who underwent cardiac surgery was continuously recruited, who were further divided into CSA‐AKI group and Non‐AKI group based on clinical outcomes. Their faecal and plasma samples were collected before surgery and were separately analysed by nontargeted and targeted metabolomics. The differential metabolites related to CSA‐AKI were screened out using statistical methods, and altered metabolic pathways were determined by examining the Kyoto Encyclopedia of Genes and Genomes database. Results Nearly 1000 faecal metabolites were detected through high‐resolution mass spectrometry (MS) and bioinformatics at high and mid confidence levels, and 49 differential metabolites at high confidence level may perform essential biological functions and provide potential diagnostic indicators. Compared with the Non‐AKI group, the patients in the CSA‐AKI group displayed dramatic changes in gut microbiota metabolism, including amino acid metabolism, nicotinate and nicotinamide metabolism, purine metabolism and ATP‐binding cassette (ABC) transporters. Meanwhile, 188 plasma metabolites were identified and quantified by tandem MS, and 34 differential plasma metabolites were screened out between the two groups using univariate statistical analysis. These differential plasma metabolites were primarily enriched in the following metabolic pathways: sulphur metabolism, amino acid biosynthesis, tryptophan metabolism and ABC transporters. Furthermore, the content of indole metabolites in the faecal and plasma samples of the CSA‐AKI group was higher than that of the Non‐AKI group. Conclusions Patients with CSA‐AKI may have dysbiosis of their intestinal microbiota and metabolic abnormalities in their gut system before cardiac surgery. Thus, some metabolites and related metabolic pathways may be potential biomarkers and new therapeutic targets for the disease.

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“…CircRNAs are related to the physiological and pathological development of many human organisms [ 12 ]. Accumulating research has revealed that circRNAs are involved in AKI, such as circ-ZNF609 [ 13 ], Circ_35953 [ 14 ], and circRNA_45478 [ 15 ]. However, a large number of circRNAs with unknown functions that are awaiting to be characterized remains AKI.…”

Section: Introductionmentioning

confidence: 99%

CircTLK1 alleviates oxygen-glucose deprivation/reperfusion induced apoptosis in HK-2 cells through miR-136-5p/Bcl2 signal axis

Kuang

Lu²,

Yao³

2023

Renal Failure

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The biological functions of circTLK1 in acute kidney injury (AKI), which mainly results from renal ischemia-reperfusion (IR), remain largely unknown. HK-2 cell treatment with oxygen and glucose deprivation, reoxygenation, and glucose (OGD/R) was used to simulate an AKI model that was mainly caused by renal IR. Then, the circTLK1 expression level in HK-2 cells treated with OGD/R was assessed by quantitative reverse transcription polymerase chain reaction (RT-qPCR). Functional experiments were performed with circTLK1 knockdown of HK-2 cells via Cell Counting Kit-8 (CCK8), flow cytometry (FCM), RT-qPCR, and western blotting. The circTLK1-miRNAs-mRNAs network was constructed following the ceRNA mechanism and visualized by Cytoscape software to investigate the mechanism of circTLK1 in AKI. RT-qPCR was performed to verify the relationship between circTLK1, miR-136-5p, and Bcl2. The level of miR-136-5p was knocked down to ensure its function in OGD/R-triggered apoptosis through experiments, including CCK8, FCM, RT-qPCR, and western blotting. CircTLK1 was downregulated in HK-2 cells subjected to OGD/R treatment and in mouse kidney tissues after renal IR, but the expression of miR-136-5p was the opposite. Interference with circTLK1 expression accelerated HK-2 cell apoptosis, which was overturned by miR-136-5p inhibitors. CircTLK1 targets miR-136-5p to upregulate Bcl2 expression and attenuate apoptosis in HK-2 cells. These data revealed the possible role of circTLK1 as a new biomarker for diagnosis as well as a target in AKI through the miR-136-5p/Bcl2 signaling axis.

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A protein encoded by circular ZNF609 RNA induces acute kidney injury by activating the AKT/mTOR-autophagy pathway

Cited by 9 publications

References 0 publications

Circular RNA in Multiple Sclerosis: Pathogenicity and Potential Biomarker Development: A Systematic Review

Circular RNA in Multiple Sclerosis: Pathogenicity and Potential Biomarker Development: A Systematic Review

Metabolomic interplay between gut microbiome and plasma metabolome in cardiac surgery‐associated acute kidney injury

CircTLK1 alleviates oxygen-glucose deprivation/reperfusion induced apoptosis in HK-2 cells through miR-136-5p/Bcl2 signal axis

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