A Protein Tyrosine Kinase Involved in Regulation of pp60c-src function

Okada, Masato; Nakagawa, Hachiro

doi:10.1016/s0021-9258(19)30019-5

Cited by 278 publications

(46 citation statements)

References 35 publications

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“…The inhibitory tyrosine was mapped to a conserved tyrosine in the C-terminal tail of c-Src [5]. Efforts to study the inhibitory effect of tyrosine phosphorylation on SFKs led to the discovery of C-terminal Src kinase (CSK) by Okada, Nakagawa and their colleagues [6][7][8][9][10]. This was followed by the discovery of CSK-homologous kinase (CHK) by several research groups in 1994 [11][12][13][14][15].…”

Section: Introduction -Csk and Chk Are The Major Endogenous Inhibitors Of Src-family Kinasesmentioning

confidence: 99%

Structural elements and allosteric mechanisms governing regulation and catalysis of CSK-family kinases and their inhibition of Src-family kinases

Mills

Hossain

et al. 2010

Growth Factors

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C-terminal Src kinase (CSK) and CSK-homologous kinase (CHK) are endogenous inhibitors constraining the activity of the oncogenic Src-family kinases (SFKs) in cells. Both kinases suppress SFKs by selectively phosphorylating their consensus C-terminal regulatory tyrosine. In addition to phosphorylation, CHK can suppress SFKs by a unique non-catalytic inhibitory mechanism that involves tight binding of CHK to SFKs to form stable complexes. In this review, we discuss how allosteric regulators, phosphorylation, and inter-domain interactions interplay to govern the activity of CSK and CHK and their ability to inhibit SFKs. In particular, based upon the published results of structural and biochemical analysis of CSK and CHK, we attempt to chart the allosteric networks in CSK and CHK that govern their catalysis and ability to inhibit SFKs. We also discuss how the published three-dimensional structure of CSK complexed with an SFK member sheds light on the structural basis of substrate recognition by protein kinases.

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Section: Introduction -Csk and Chk Are The Major Endogenous Inhibitors Of Src-family Kinasesmentioning

confidence: 99%

Structural elements and allosteric mechanisms governing regulation and catalysis of CSK-family kinases and their inhibition of Src-family kinases

Mills

Hossain

et al. 2010

Growth Factors

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“…1). The discovery of Csk can be traced back to an important study performed two decades ago (Okada and Nakagawa 1989). The study showed that Tyr-527 of c-Src did not undergo autophosphorylation.…”

Section: Discussionmentioning

confidence: 99%

CD283 Antigen

2018

Encyclopedia of Signaling Molecules

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Crk SH3 domain binding guanine nucleotide release protein (C3G), Rap guanine nucleotide exchange factor 1 (RAPGEF1), Guanine nucleotide-releasing factor 2 (GRF2) 620 C3G 622 C3G C3G 623 C Downregulated Fernandez et al. 2008 Lymphoproliferative disorder Mutation, Y572C Parker et al. 2016 Human prostate carcinoma Regulates PLCg1-mediated adhesion Peak et al. 2008 Juvenile neuronal ceroid lipofuscinosis Upregulated Lebrun et al. 2011 624 C3G fate determinant. While its catalytic activity enables activation of small GTPases of Ras family, it also has cellular roles independent of its catalytic activity. The fact that other Rap1 GEFs do not compliment C3G during mammalian embryonic development suggests important noncatalytic functions, or its ability to activate other GTPases. Recent studies have shown that altered C3G levels are associated with human cancers and other disorders, suggesting its role in maintaining homeostasis in adult tissues.

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“…src , we exa mined whether MATK ph osphorylated p60e-src wit hout kin ase activity. For this, p60e-src was treated wit h an ATP a nalogue, FSBA , which is kn own to in activate p60e-src by reacting wit h lysin e 295 (20). Almost all the kinase activity of p60e-src was destroyed by incubation wit h 1.0 mxi FSBA for 60 min at 30°C.…”

Section: Discussionmentioning

confidence: 99%

“…Biochemical Analyses-For preparation of proteins for immunoprecipitation, proteins from CMK and Dami cells were lysed in modified RIPA buffer (20,23). One J-Lg of appropriate antibody was added to each protein sample (10 J-Lg).…”

Section: 'mentioning

confidence: 99%

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Structural and Functional Studies of the Intracellular Tyrosine Kinase MATK Gene and Its Translated Product

Avraham

Jiang

Ota

et al. 1995

Journal of Biological Chemistry

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We recently cloned the cDNA which encodes a novel megakaryocyte-associated tyrosine kinase termed MATK. In this study, we have cloned and characterized the human MATK gene as well as the murine homolog of human MATK cDNA and performed functional studies of its translated product. Comparison of the deduced amino acid sequences of human and murine MATK cDNAs revealed 85% homology, indicating that MATK is highly conserved in mouse and human. The human gene consists of 13 exons interrupted by 12 introns. The genetic units which encode the SH3 and SH2 domains are located on separate exons. The putative ATP binding site (GXGXXG) is localized on exon 7, and the entire catalytic domain is subdivided into seven exons (7-13). Somatic cell hybrid analysis indicated that human MATK gene is located on chromosome 19 while the murine Matk gene is located on chromosome 10. The immediate 5'-flanking region was highly rich in GC sequences, and potential cis-acting elements were identified including several SP1, GATA-1, APRE, and APRE1. Antisense oligonucleotides directed against MATK mRNA sequences significantly inhibited megakaryocyte progenitor proliferation. Functional studies indicated that MATK can phosphorylate the carboxyl-terminal conserved tyrosine of the Src protein. These results support the notion that MATK acts as a regulator of p60c-src in megakaryocytic cells and participates in the pathways regulating growth of cells of this lineage.

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A Protein Tyrosine Kinase Involved in Regulation of pp60c-src function

Cited by 278 publications

References 35 publications

Structural elements and allosteric mechanisms governing regulation and catalysis of CSK-family kinases and their inhibition of Src-family kinases

Structural elements and allosteric mechanisms governing regulation and catalysis of CSK-family kinases and their inhibition of Src-family kinases

CD283 Antigen

Structural and Functional Studies of the Intracellular Tyrosine Kinase MATK Gene and Its Translated Product

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