2012
DOI: 10.1186/1477-5956-10-20
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A proteome map of primary cultured rat Schwann cells

Abstract: BackgroundSchwann cells (SCs) are the principal glial cells of the peripheral nervous system with a wide range of biological functions. SCs play a key role in peripheral nerve regeneration and are involved in several hereditary peripheral neuropathies. The objective of this study was to gain new insight into the whole protein composition of SCs.ResultsTwo-dimensional liquid chromatography coupled with tandem mass spectrometry (2D LC-MS/MS) was performed to identify the protein expressions in primary cultured S… Show more

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Cited by 19 publications
(16 citation statements)
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“…The most prominent difference between human and rodent SC cultures was the low expression of GFAP in human SCs. GFAP upregulation generally characterizes the immature state of rodent SC cultures and has also been linked to repair SCs in nerve regeneration (Kaewkhaw et al, ; Shen et al, ; Triolo et al, ). However, GFAP was not upregulated in human ex vivo degenerated peripheral nerves.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The most prominent difference between human and rodent SC cultures was the low expression of GFAP in human SCs. GFAP upregulation generally characterizes the immature state of rodent SC cultures and has also been linked to repair SCs in nerve regeneration (Kaewkhaw et al, ; Shen et al, ; Triolo et al, ). However, GFAP was not upregulated in human ex vivo degenerated peripheral nerves.…”
Section: Discussionmentioning
confidence: 99%
“…So far, expression analyses of nerve repair‐related processes have primarily been obtained from rodent tissue derived SC cultures in vitro (Shen et al, ; Ulrich et al, ) and peripheral nerves in situ or ex vivo (Fontana et al, ; Gomez‐Sanchez et al, ; Jimenez et al, ). This raises the question whether findings are readily transferrable to the human system.…”
Section: Introductionmentioning
confidence: 99%
“…The last decade of neuroproteomics research has resulted in a wealth of information on CNS protein profiles, and the proteomes of microglia [96,102,107,110,252], astrocytes [39,74,206,[253][254][255][256][257][258], and of other glial cell types [259][260][261][262][263] have been extensively analyzed with the intent of discovering biomarkers and therapeutic targets for CNS disorders. Glial proteins that are being studied as potential novel biomarkers include pentraxin-3 [102], serum amyloid A [264], cathepsin D [265], chitinase 3-like 1 (CHI3L1) [255,266], plasminogen activator inhibitor type 1 [267], metallothioneins [268], LCN2 [143,149,269], LCN-type prostaglandin D synthase, and apo D [270][271][272].…”
Section: Glioproteomics: a Methods Of Choice For The Discovery Of Biommentioning
confidence: 99%
“…Minimization of the number of contaminating fibroblasts, which can affect the biological analysis and experimentation of SCs, and increase scar tissue formation, is required. For this purpose, the present study modified the techniques of several previously published protocols and developed a method for the isolation and enrichment of rat SCs from sciatic nerves ( 9 14 ).…”
Section: Introductionmentioning
confidence: 99%
“…Therefore, several methods exist to separately remove either fibroblast cells from the SC cultures or SCs from fibroblasts, as a form of purification ( 15 ). The use of antimitotic chemicals is a commonly used technique to inhibit fibroblast growth on the basis of the higher proliferation rate of fibroblasts ( 9 ). Furthermore, the preferential surface expression of Thy-1 by fibroblast cells can be exploited by using anti-Thy1 antibodies, in conjunction with complement-mediated cell lysis ( 16 ).…”
Section: Introductionmentioning
confidence: 99%