A proteomic and RNA-seq transcriptomic dataset of capsaicin-aggravated mouse chronic colitis model

Chen, Kexin; Shen, Shunyao; Chen, Yiding; Jiang, Meng; Hu, Kehan; Zou, Yuheng; Li, Lili; Zeng, Zhen; Ma, Chaoqiong; Dang, Yuan; Hu, Zhang

doi:10.1038/s41597-022-01637-3

Cited by 6 publications

(3 citation statements)

References 34 publications

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“…In addition, capsaicin can activate TRPV1 channels and has been shown to decrease DSS-induced colitis in rats when delivered subcutaneously [ 46 ]. In contrast, orally consumed capsaicin appears to be able to induce or worsen chronic colitis in some models [ 47 ]. This may be due to capsaicin inducing dysbiosis—specifically, reducing Firmicutes and increasing Bacteroides—resulting in intestinal permeability and the translocation of gut pathogen molecules to exacerbate inflammation [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effects of Serotonin Receptor and Transient Receptor Potential Channel Ligands in Human Small Intestinal Epithelial Cells

Robinson,

Li,

Wang

et al. 2023

CIMB

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Intestinal inflammation and dysbiosis can lead to inflammatory bowel diseases (IBD) and systemic inflammation, affecting multiple organs. Developing novel anti-inflammatory therapeutics is crucial for preventing IBD progression. Serotonin receptor type 2A (5-HT2A) ligands, including psilocybin (Psi), 4-Acetoxy-N,N-dimethyltryptamine (4-AcO-DMT), and ketanserin (Ket), along with transient receptor potential (TRP) channel ligands like capsaicin (Cap), curcumin (Cur), and eugenol (Eug), show promise as anti-inflammatory agents. In this study, we investigated the cytotoxic and anti-inflammatory effects of Psi, 4-AcO-DMT, Ket, Cap, Cur, and Eug on human small intestinal epithelial cells (HSEIC). HSEIC were exposed to tumor necrosis factor (TNF)-α and interferon (IFN)-γ for 24 h to induce an inflammatory response, followed by treatment with each compound at varying doses (0–800 μM) for 24 to 96 h. The cytotoxicity was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and protein expression by Western blot (WB) analysis. As single treatments, Psi (40 μM), Cur (0.5 μM), and Eug (50 μM) significantly reduced COX-2 levels without cytotoxic effects. When combined, Psi (40 μM) and Cur (0.5 μM) exhibited synergy, resulting in a substantial decrease in COX-2 protein levels (−28× fold change), although the reduction in IL-6 was less pronounced (−1.6× fold change). Psi (20 μM) and Eug (25 μM) demonstrated the most favorable outcomes, with significant decreases in COX-2 (−19× fold change) and IL-6 (−10× fold change) protein levels. Moreover, the combination of Psi and Eug did not induce cytotoxic effects in vitro at any tested doses. This study is the first to explore the anti-inflammatory potential of psilocybin and 4-AcO-DMT in the intestines while highlighting the potential for synergy between the 5-HT2A and TRP channel ligands, specifically Psi and Eug, in alleviating the TNF-α/IFN-γ-induced inflammatory response in HSEIC. Further investigations should evaluate if the Psi and Eug combination has the therapeutic potential to treat IBD in vivo.

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Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effects of Serotonin Receptor and Transient Receptor Potential Channel Ligands in Human Small Intestinal Epithelial Cells

Robinson,

Li,

Wang

et al. 2023

CIMB

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“…Therefore, capsaicin may be used as a new active ingredient to treat sepsis and inflammation. Chen and colleagues gathered proteomic data in the colon tissues of capsaicin-treated mice with chronic colitis (42). A total of 2,763 proteins were detected using MS, among which several differentially expressed proteins were found closely associated with inflammatory pathways.…”

Section: Effects Of Capsaicin On Inflammatory-related Metabolic Pathwaymentioning

confidence: 99%

Recent advances in analysis of capsaicin and its effects on metabolic pathways by mass spectrometry

Peng

Zhang

et al. 2023

Front. Nutr.

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Capsaicin is the main food active component in Capsicum that has gained considerable attention due to its broad biological activities, including antioxidation, anti-inflammation, anti-tumor, weight regulation, cardiac protection, anti-calculi, and diurnal-circadian regulation. The potent biological effects of capsaicin are intimately related to metabolic pathways such as lipid metabolism, energy metabolism, and antioxidant stress. Mass spectrometry (MS) has emerged as an effective tool for deciphering the mechanisms underlying capsaicin metabolism and its biological impacts. However, it remains challenging to accurately identify and quantify capsaicin and its self-metabolites in complex food and biological samples, and to integrate multi-omics data generated from MS. In this work, we summarized recent advances in the detection of capsaicin and its self-metabolites using MS and discussed the relevant MS-based studies of metabolic pathways. Furthermore, we discussed current issues and future directions in this field. In-depth studies of capsaicin metabolism and its physiological functions based on MS is anticipated to yield new insights and methods for preventing and treating a wide range of diseases.

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“…IBD has become a global disease with the highest prevalence in Westernized countries and the greatest growing incidence in newly industrialized countries [ 7 ]. The disease puts a heavy burden not only on patients themselves and their families, but also on health care systems [ 8 ]. Substantial evidence indicates that IBD results from the interaction of genetic/epigenetic, environmental, immunological, and microbial aspects.…”

Section: Introductionmentioning

confidence: 99%

Precision medicine in inflammatory bowel disease

Zeng,

Jiang,

et al. 2023

Precision Clinical Medicine

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Inflammatory bowel disease (IBD) is an incurable disease characterized by remission-relapse cycles throughout its course. Both Crohn's disease (CD) and ulcerative colitis (UC), the two main forms of IBD, exhibit tendency to develop complications and substantial heterogeneity in terms of frequency and severity of relapse, thus posing great challenges to the clinical management for IBD. Current treatment strategies are effective in different ways in induction and maintenance therapies for IBD. Recent advances in studies of genetics, pharmacogenetics, proteomics and microbiome provide a strong driving force for identifying molecular markers of prognosis and treatment response, which should help clinicians manage IBD patients more effectively, and then, improve clinical outcomes and reduce treatment costs of patients. In this review, we summarize and discuss precision medicine in IBD, focusing on predictive markers of disease course and treatment response, and monitoring indices during therapeutic drug monitoring.

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A proteomic and RNA-seq transcriptomic dataset of capsaicin-aggravated mouse chronic colitis model

Cited by 6 publications

References 34 publications

Anti-Inflammatory Effects of Serotonin Receptor and Transient Receptor Potential Channel Ligands in Human Small Intestinal Epithelial Cells

Anti-Inflammatory Effects of Serotonin Receptor and Transient Receptor Potential Channel Ligands in Human Small Intestinal Epithelial Cells

Recent advances in analysis of capsaicin and its effects on metabolic pathways by mass spectrometry

Precision medicine in inflammatory bowel disease

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