A proteomic approach reveals biomineralization and immune response for mantle to pearl sac in the freshwater pearl mussel (Hyriopsis cumingii)

Li, Xue-Nan; Feng, Shangle; Xuan, Xingrong; Wang, He; Shen, Xiaoya; Chen, Yige; Fu, Yuanshuai; Bai, Zhiyi; Li, Wenjuan

doi:10.1016/j.fsi.2022.06.057

Cited by 8 publications

(4 citation statements)

References 30 publications

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“…The regulation of the release of inflammatory mediators and apoptosis may be beneficial to the healing of epithelial cells and the formation of the pearl sac after insertion (Li et al, 2022a). In this study, the lipopolysaccharide-binding protein, serine/threonineprotein kinase NLK, antileukoproteinase (fragment), nuclear factor NF-kappa-B p105 subunit, and fibrillin-1 were related to inflammatory responses and cell apoptosis and were differentially expressed.…”

Section: Discussionmentioning

confidence: 70%

“…The immune system of invertebrates differs from that of vertebrates in that they only show innate immunity and no acquired immunity. As invertebrates, mollusks have no specific immune cells and corresponding antibodies and rely on humoral factors and hemocytes in the hemolymph to defend against foreign invasion, so-called 'humoral and cellular immunity' (Li et al, 2022a;Roch, 1999). One of the earliest immune system components, the complement system is crucial to both innate and acquired immunity (Hajishengallis et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

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Transcriptome and digital gene expression analysis reveal immune responses of mantle and visceral mass pearl culturing in Hyriopsis cumingii

Shen,

Chen,

Jia

et al. 2023

Front. Mar. Sci.

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Biomineralization is a widespread phenomenon in marine mollusks and is responsible for the production of shells and pearls. However, the regulatory mechanisms governing the adaptive immune responses in the mollusk mantle and visceral mass during mineralization remain unclear. In this work, we examined the mantle and visceral mass immune responses of Hyriopsis cumingii during pearl culture using high-throughput sequencing techniques. A mantle transcriptome database was established using transcriptome sequencing technology and reference to the major databases. Digital gene expression profiling was used to identify the differentially expressed genes of mantle and visceral mass at different insertion periods. Moreover, quantitative real-time PCR was used to verify the expression of five immune-related genes. Transcriptome sequencing results showed 257,457 unigenes were identified. Digital gene expression profiles showed 1389, 3572, 1888, and 2613 differentially expressed genes (DEGs) in the mantle and visceral mass at 5, 20, 50, and 90 d after insertion, respectively, with the highest number at 20 d and the lowest at 5 d after insertion (q < 0.05). A cluster analysis of the DEGs showed similar clustering and expression features in the mantle to the control group, and at 5, 50 and 90 d, after mantle insertion. The DEGs in the visceral mass showed similar clustering and expression features to the control group and at 5, 20 and 50 d after insertion. We also screened 22 immune-related DEGs in the mantle and visceral mass during the same pearl culture period, including serine/threonine-protein kinase NLK, C-type lectin, and galectin. The greatest number of DEGs was found 90 d after insertion. Compared with the mantle, more immune-related DEGs were down-regulated than up-regulated in the visceral mass during pearl culture, indicating that the immune regulatory mechanisms in the visceral mass and the mantle differ during pearl culture, and that the visceral mass is liable to higher infection and mortality rates. Quantitative real-time PCR results showed that the expression of five immune-related genes was consistent with DGE results. Our findings will further knowledge of the immune systems that are present in the mantle and visceral mass during pearl culture.

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Section: Discussionmentioning

confidence: 70%

Section: Discussionmentioning

confidence: 99%

Transcriptome and digital gene expression analysis reveal immune responses of mantle and visceral mass pearl culturing in Hyriopsis cumingii

Shen,

Chen,

Jia

et al. 2023

Front. Mar. Sci.

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“…Proteome Discoverer (Thermo Electron, version 2.2) was used for protein identification. The transcriptome database of H.cumingii mantle (30,31) and FBS (https://www.ncbi.nlm.nih.gov) was used as a reference for the analysis. In protein quantification, the proteins must contain unique peptides.…”

Section: Liquid Chromatography-mass Spectrometry Analysismentioning

confidence: 99%

Presence of immune factors in freshwater mussel (Hyriopsis cumingii) entails autologous serum an essential component in the culture of mantle cells

Bai

Wang

et al. 2023

Front. Immunol.

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Mussel cell culture is a challenging problem and serum serves a crucial biological role in cell culture as an autologous supply and an immunizing agent. In this study, the biology (calcium ions, total protein, pH, and osmotic pressure) of fetal bovine serum (FBS) and Hyriopsis cumingii serum (HCS) was investigated, and the development of Hyriopsis cumingii (H. cumingii) mantle cells in HCS and FBS systems was examined. The results showed that total protein, calcium ions, and osmotic pressure varied significantly (p<0.05). The activity of mantle cells was superior in the HCS culture system to that in the FBS culture system. The label-free technique was used to distinguish the two serum proteins to investigate the supportive effect of autologous serum on cell culture. These were examined for 109 unique proteins and 35 particular HCS proteins. Most differentially expressed proteins (DEPs) were involved in immune response, cell differentiation, and calcium ion binding. Furthermore, immune factors such as HSP, CALR, APOB, C3 were identified with significant differences. HSP was significantly more present in HCS than in FBS as an endogenous protective protein that regulates immune system function, cell differentiation, transport, and activity regulation. Parallel reaction monitoring (PRM) analysis was carried out to validate the expression levels of 19 DEPs, indicating high reliability of the proteomic results. This study reveals the important role of immune factors in mussel cell culture, providing a theoretical basis for explaining the applicability of autologous serum in cell culture. It is also helpful in improving the cell culture conditions of mussels.

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“…Notably, the mantle is not only a tissue for shell formation but also an immune-related tissue in the mussel innate immune system (Huang et al, 2022;Li et al, 2022). Previous studies have demonstrated that elevated pCO 2 has a significant effect on the immune system of Pacific oysters, with the mRNA expression patterns of several immune-related genes varying depending on the exposure time and tissues (Wang et al, 2016).…”

Section: Expression Of Immune-related and Energy-related Transcriptsmentioning

confidence: 99%

Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage

Liao,

Liu,

Wang

et al. 2023

Front. Physiol.

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Mytilus coruscus is an economically important marine calcifier living in the Yangtze River estuary sea area, where seasonal fluctuations in natural pH occur owing to freshwater input, resulting in a rapid reduction in seawater pH. In addition, Mytilus constantly suffers from shell fracture or injury in the natural environment, and the shell repair mechanisms in mussels have evolved to counteract shell injury. Therefore, we utilized shell-complete and shell-damaged Mytilus coruscus in this study and performed transcriptomic analysis of the mantle to investigate whether the expression of mantle-specific genes can be induced by acute seawater acidification and how the mantle responds to acute acidification during the shell repair process. We found that acute acidification induced more differentially expressed genes than shell damage in the mantle, and the biomineralization-related Gene Ontology terms and KEGG pathways were significantly enriched by these DEGs. Most DEGs were upregulated in enriched pathways, indicating the activation of biomineralization-related processes in the mussel mantle under acute acidification. The expression levels of some shell matrix proteins and antimicrobial peptides increased under acute acidification and/or shell damage, suggesting the molecular modulation of the mantle for the preparation and activation of the shell repairing and anti-infection under adverse environmental conditions. In addition, morphological and microstructural analyses were performed for the mantle edge and shell cross-section, and changes in the mantle secretory capacity and shell inner film system induced by the two stressors were observed. Our findings highlight the adaptation of M. coruscus in estuarine areas with dramatic fluctuations in pH and may prove instrumental in its ability to survive ocean acidification.

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A proteomic approach reveals biomineralization and immune response for mantle to pearl sac in the freshwater pearl mussel (Hyriopsis cumingii)

Cited by 8 publications

References 30 publications

Transcriptome and digital gene expression analysis reveal immune responses of mantle and visceral mass pearl culturing in Hyriopsis cumingii

Transcriptome and digital gene expression analysis reveal immune responses of mantle and visceral mass pearl culturing in Hyriopsis cumingii

Presence of immune factors in freshwater mussel (Hyriopsis cumingii) entails autologous serum an essential component in the culture of mantle cells

Transcriptomic response of Mytilus coruscus mantle to acute sea water acidification and shell damage

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