A Proteomic Approach to Study of the Function of the Sec14P-Like p45 Protein

Abstract

We undertook a search for proteins interacting with protein p45 by the method of two-hybrid screening in order to determine the function of the Sec14p-like protein p45. A screening of the yeast library of rat lung cDNA, six proteins specifically activating the reporter genes of a two-hybrid system and 21 unlikely protein partners of protein p45 were identified. The most likely candidate for the role of a p45 partner is the surfactant protein C (Sftpc). These results and previous studies led us to the hypothesis that protein p45 fulfills its protective function by participating in the biogenesis of cell membranes. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2005, vol. 31, no. 3; see also http://www.maik.ru.

A New Method for Studying in vitro Lipid Transport: The 45-kDa Protein from Rat Olfactory Epithelium Is a Specific Carrier of Phosphatidylinositol 3,4,5-Triphosphate

“…Here we report a new approach to the detection of unknown lipid ligands of proteins and the study of lipid transport in vitro by the example of detection of the PtdIns(3,4,5) P 3 -transporting function of the 45-kDa protein from the rat olfactory epithelium (p45) [7,8].…”