2021
DOI: 10.3390/ijms22157957
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A Proteomic Study Suggests Stress Granules as New Potential Actors in Radiation-Induced Bystander Effects

Abstract: Besides the direct effects of radiations, indirect effects are observed within the surrounding non-irradiated area; irradiated cells relay stress signals in this close proximity, inducing the so-called radiation-induced bystander effect. These signals received by neighboring unirradiated cells induce specific responses similar with those of direct irradiated cells. To understand the cellular response of bystander cells, we performed a 2D gel-based proteomic study of the chondrocytes receiving the conditioned m… Show more

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Cited by 7 publications
(12 citation statements)
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“…X-ray irradiations were performed with a PxiXradSmart 225cX irradiator using a tube tension of 225 kV, as previously described [21][22][23][24][25][26][27][28][29][30], and C-ion irradiations were performed in the GANIL facility (Caen, France) using the high-energy beam line IRABAT, as previously described [31,32]. For C-ions, a LET of 73 keV/µm was selected to reproduce clinical conditions.…”
Section: Irradiationmentioning
confidence: 99%
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“…X-ray irradiations were performed with a PxiXradSmart 225cX irradiator using a tube tension of 225 kV, as previously described [21][22][23][24][25][26][27][28][29][30], and C-ion irradiations were performed in the GANIL facility (Caen, France) using the high-energy beam line IRABAT, as previously described [31,32]. For C-ions, a LET of 73 keV/µm was selected to reproduce clinical conditions.…”
Section: Irradiationmentioning
confidence: 99%
“…Proteins were extracted from bystander chondrocytes (dry pellet) in lysis buffer (RIPA made without protease, in order not to interfere with proteases mass spectrometry) and left on ice for 20 min [29]. Mechanical grinding was carried out using a hand-held motorized pellet pestle for 20 s on ice, followed by 20 min at rest.…”
Section: Protein Sample Preparationmentioning
confidence: 99%
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