A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning

Teulé, Florence; Cooper, Alyssa R.; Furin, William A; Bittencourt, Daniela; Rech, E. L.; Brooks, Amanda E.; Lewis, Randolph V.

doi:10.1038/nprot.2008.250

Cited by 172 publications

(204 citation statements)

References 50 publications

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“…To quantitatively estimate the stress experienced by the fiber in the constriction, a model, inspired from models of the scission of polymer chains in elongational flowfields, is proposed (8). No eddies are observed and the flow is considered as laminar.…”

Section: Discussionmentioning

confidence: 99%

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Kinetics of fiber solidification

Mercader

Lucas

Derré

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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Many synthetic or natural fibers are produced via the transformation of a liquid solution into a solid filament, which allows the wet processing of high molecular weight polymers, proteins, or inorganic particles. Synthetic wet-spun fibers are used in our everyday life from clothing to composite reinforcement applications. Spun fibers are also common in nature. Silk solidification results from the coagulation of protein solutions. The chemical phenomena involved in the formation of all these classes of fibers can be quite different but they all share the same fundamental transformation from a liquid to a solid state. The solidification process is critical because it governs the production rate and the strength that fibers can sustain to be drawn and wound. An approach is proposed in this work to investigate the kinetics of fiber solidification. This approach consists in circulating solidifying fibers in the extensional flow of a surrounding liquid. Such as polymers in extensional flows, the fibers break if resultant drag forces exceed the fiber tensile strength. The solidification kinetics of nanotube composite fibers serves as a validation example of this approach. The method could be extended to other systems and advance thereby the science and technology of fiber and textile materials. It is also a way to directly visualize the scission of chain-like systems in extensional flows.diffusion | elongational | microfluidic | rheology

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Section: Discussionmentioning

confidence: 99%

“…Natural or synthetic polymer solidification can indeed result from various phenomena including dehydration, injection in poor solvents, pH variations, temperature variations, phase transitions, etc. (2)(3)(4)(5)(6)(7)(8)(9). The kinetics of solidification is primarily limited by the diffusion of the solvent, additives, and polymer molecules.…”

mentioning

confidence: 99%

Kinetics of fiber solidification

Mercader

Lucas

Derré

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

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show abstract

“…Heterologous silk protein studies have been conducted using a range of organisms including bacteria, 78 tobacco plants, 79 yeast, 80 silkworms, 81 goats, 82 and mammalian cells 83 (for an in-depth review, see Refs. 9 and 52).…”

Section: A Making Synthetic Silkmentioning

confidence: 99%

“…27 The distinction is the subject of quite some philosophical scrutiny. 51 Here we discuss primarily biosynthetic recombinant spider silks, which are produced by suitable hosts and then purified and processed into fibers via extrusion of a dope solution, 78 and regenerated natural silks, in which natural silk proteins are extracted, chemically treated, and extruded. 72 Fibers formed from regenerated natural silks have generally exhibited mechanical performance that compares unfavorably with natural spider silk, regardless of whether the regenerated silk was formed from aqueous solution 107 or from organic solvents.…”

Section: Overcoming the Mechanical Performance Deficitmentioning

confidence: 99%

With great structure comes great functionality: Understanding and emulating spider silk

et al. 2014

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The overarching aim of biomimetic approaches to materials synthesis is to mimic simultaneously the structure and function of a natural material, in such a way that these functional properties can be systematically tailored and optimized. In the case of synthetic spider silk fibers, to date functionalities have largely focused on mechanical properties. A rapidly expanding body of literature documents this work, building on the emerging knowledge of structure-function relationships in native spider silks, and the spinning processes used to create them. Here, we describe some of the benchmark achievements reported until now, with a focus on the last five years. Progress in protein synthesis, notably the expression on full-size spidroins, has driven substantial improvements in synthetic spider silk performance. Spinning technology, however, lags behind and is a major limiting factor in biomimetic production. We also discuss applications for synthetic silk that primarily capitalize on its nonmechanical attributes, and that exploit the remarkable range of structures that can be formed from a synthetic silk feedstock.

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“…The morphological features and performance of silk fibers are dependent on the coagulation conditions, such as the temperature, pH and coagulant type and rate, that are used to form the fibers. 6,26,27 Purified aneroin proteins were fully dissolved in HFIP and then extruded through a thin needle into a reservoir containing various organic solvents. The ability of the fibers to be spun was tested under various conditions by monitoring the breakage of the fibers and the microscale morphology of the resulting fibers (data not shown).…”

Section: Sequence and Immunohistochemical Analysesmentioning

confidence: 99%

Production of a novel silk-like protein from sea anemone and fabrication of wet-spun and electrospun marine-derived silk fibers

et al. 2013

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Silk-based biomaterials, which for centuries were primarily used as the raw materials for textile fabrics, are now being used in the medical, food and industrial fields because of their extraordinary properties. Their advantageous characteristics include high strength, extensibility, biocompatibility, biodegradability and minimal production of inflammatory reactions. To date, only silkworm silk has been applied in mass-produced technological applications, but spider silk proteins have been extensively studied because of their superior mechanical stability. Here we report the discovery of a novel silk-like protein (named aneroin) from the sea anemone Nematostella vectensis and the successful fabrication of wet-spun and electrospun silk fibers from purified recombinant aneroins. Aneroin fibers have promising mechanical properties, similar to those of recombinant spider silks with molecular weights of B100 kDa and those of natural mammalian tendon collagen. The results of this study demonstrate that aneroin, a new repertoire of silk-like protein found in sea anemones, has potential for use as a novel fibrous biomaterial. Its use would expand the applications of silk in the development of multifunctional and bio-inspired materials.

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A protocol for the production of recombinant spider silk-like proteins for artificial fiber spinning

Cited by 172 publications

References 50 publications

Kinetics of fiber solidification

Kinetics of fiber solidification

With great structure comes great functionality: Understanding and emulating spider silk

Production of a novel silk-like protein from sea anemone and fabrication of wet-spun and electrospun marine-derived silk fibers

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