A proton nuclear magnetic resonance study of the binding of methylmercury in human erythrocytes

Rabenstein, Dallas L.; Isab, Anvarhusein A.; Reid, R. S.

doi:10.1016/0167-4889(82)90038-6

Cited by 84 publications

(40 citation statements)

References 18 publications

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“…There are no resonances which could be assigned to a dimethylarsinic(II1) moiety, which would be expected approximately 0.5 ppm upfield of the parent acid (11). The cysteinyl group (g2) of glutathione does not indicate any binding of arsenic through the sulfur (18). Therefore the reduction of dimethylarsinic acid by glutathione is unlikely to occur by direct reaction between the two compounds although it may occur indirectly via some membrane-mediated process (i.e., the arsenic acid remains extracellular).…”

Section: Resultsmentioning

confidence: 94%

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Spin‐echo ¹H NMR detected response of ergothioneine to oxidative stress in the intact human erythrocyte

Reglinski

Smith

Sturrock

1988

Magnetic Resonance in Med

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Human erythrocytes were subjected to oxidative stress using arsenicals. The study is a model for thiol-related redox processes observed in the etiology of diseases such as rheumatoid arthritis. Spin-echo NMR spectroscopy of the living cell was used to monitor the cellular biochemistry. Oxidation of glutathione and the first demonstrated response of ergothioneine to a chemical stimulus in a cell were observed. The reversible nature of the ergothioneine response is interpreted as an environmental rather than a chemical change.

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Section: Resultsmentioning

confidence: 94%

“…Thus, the intensity and sign of this signal is a direct measure of the redox status of this molecule. Furthermore, direct binding of species like methylmercury to glutathione through the P-methylene group produces a specific change (nulling) in this residue (18), again indicating the sensitivity of this signal to molecular change.…”

Section: Resultsmentioning

confidence: 98%

Spin‐echo ¹H NMR detected response of ergothioneine to oxidative stress in the intact human erythrocyte

Reglinski

Smith

Sturrock

1988

Magnetic Resonance in Med

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“…Previous studies have estimated binding affinities of MMHg to small amino acids including glutamic acid and alanine in both laboratory settings (Corbeil et al, 1986;Alex and Savoie, 1987) and by using biological media such as human erythrocytes (Rabenstein et al, 1982). These studies have demonstrated that, while MMHg demonstrates appreciable binding affinity with these amino acids (Corbeil et al, 1986;Alex and Savoie, 1987), the binding affinity is weak enough to lessen MMHg transport to various biological tissues when compared to thiol-ligands (Rabenstein et al, 1982). Other amino acids examined by Bradley et al (2014) (i.e., glycine, lysine, serine, proline) displayed equally weak binding affinity with MMHg (Alex and Savoie, 1987).…”

Section: And Dmentioning

confidence: 99%

Quantifying mercury isotope dynamics in captive Pacific bluefin tuna (Thunnus orientalis)

Kwon

Blum

Madigan

et al. 2016

Elementa: Science of the Anthropocene

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Analyses of mercury (Hg) isotope ratios in fish tissues are used increasingly to infer sources and biogeochemical processes of Hg in natural aquatic ecosystems. Controlled experiments that can couple internal Hg isotope behavior with traditional isotope tracers (d 13 C, d15 N) can improve the applicability of Hg isotopes as natural ecological tracers. In this study, we investigated changes in Hg isotope ratios (d 202 Hg, D 199 Hg) during bioaccumulation of natural diets in the pelagic Pacific bluefin tuna (Thunnus orientalis; PBFT). Juvenile PBFT were fed a mixture of natural prey and a dietary supplement (60% Loligo opalescens, 31% Sardinops sagax, 9% gel supplement) in captivity for 2914 days, and white muscle tissues were analyzed for Hg isotope ratios and compared to time in captivity and internal turnover of d 13 C and d 15 N. PBFT muscle tissues equilibrated to Hg isotope ratios of the dietary mixture within ∼700 days, after which we observed a cessation in further shifts in D

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“…It can be metabolised (degraded) or it can interact with the cell macrostructure. Should the latter occur, the molecule will change relaxation time and would subsequently be expected to be filtered from the spectrum (Rabenstein et al, 1982). two effects on the HeLa cell.…”

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confidence: 99%

The use of 1H spin echo NMR and HPLC to confirm doxorubicin induced depletion of glutathione in the intact HeLa cell

Al-Kabban

Watson²,

Stewart³

et al. 1988

Br J Cancer

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Summary The effects of doxorubicin on the cellular biochemistry of the HeLa cell using 1H spin echo nuclear magnetic resonance spectroscopy (NMR) of the intact and viable cell in conjunction with dual wavelength HPLC of cell lysates is reported. Directly dose-related changes were observed in lactate and reduced glutathione concentration. Doxorubicin induces a time-dependent depletion of the cytosolic pool of glutathione and a change in the glycolytic pattern of the cell. The glutathione depletion could be partially reversed by controlled pre-treatment of the cells with N-acetylcysteine and cysteine, the protection being linked to the intracellular concentration of the thiol.

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A proton nuclear magnetic resonance study of the binding of methylmercury in human erythrocytes

Cited by 84 publications

References 18 publications

Spin‐echo ¹H NMR detected response of ergothioneine to oxidative stress in the intact human erythrocyte

Spin‐echo ¹H NMR detected response of ergothioneine to oxidative stress in the intact human erythrocyte

Quantifying mercury isotope dynamics in captive Pacific bluefin tuna (Thunnus orientalis)

The use of 1H spin echo NMR and HPLC to confirm doxorubicin induced depletion of glutathione in the intact HeLa cell

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A proton nuclear magnetic resonance study of the binding of methylmercury in human erythrocytes

Cited by 84 publications

References 18 publications

Spin‐echo 1H NMR detected response of ergothioneine to oxidative stress in the intact human erythrocyte

Spin‐echo 1H NMR detected response of ergothioneine to oxidative stress in the intact human erythrocyte

Quantifying mercury isotope dynamics in captive Pacific bluefin tuna (Thunnus orientalis)

The use of 1H spin echo NMR and HPLC to confirm doxorubicin induced depletion of glutathione in the intact HeLa cell

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Spin‐echo ¹H NMR detected response of ergothioneine to oxidative stress in the intact human erythrocyte

Spin‐echo ¹H NMR detected response of ergothioneine to oxidative stress in the intact human erythrocyte