A Purified Recombinant Lipopeptide as Adjuvant for Cancer Immunotherapy

Song, Ying-Chyi; Liu, Hsueh-Hung; Chen, I-Hua; Chen, Hsin–Wei; Chong, Pele; Leng, Chih‐Hsiang; Liu, Shih‐Jen

doi:10.1155/2014/349783

Cited by 7 publications

(7 citation statements)

References 37 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polymyxin binds to LPS in Gram negative bacteria by electrostatic interaction by involving its N-terminal fatty acid tail that leads to its bactericidal action because of inhibition of synthesis of outer membrane [ 7 ]. Synthetic lipopeptides are widely used as vaccine adjuvants to enhance immune response, but bacterial derived recombinant lipopeptide, such as Lipo-Nter, is a novel adjuvant that can be used to induce superior antitumor effects as compared to a synthetic lipopeptide [ 8 ]. The principal representative of the lipopeptide family is Surfactin, which is produced by a bacterium Bacillus subtilis .…”

Section: Introductionmentioning

confidence: 99%

Lipopeptides as the Antifungal and Antibacterial Agents: Applications in Food Safety and Therapeutics

Meena

Kanwar

2015

BioMed Research International

340

203

View full text Add to dashboard Cite

A lot of crops are destroyed by the phytopathogens such as fungi, bacteria, and yeast leading to economic losses to the farmers. Members of the Bacillus genus are considered as the factories for the production of biologically active molecules that are potential inhibitors of growth of phytopathogens. Plant diseases constitute an emerging threat to global food security. Many of the currently available antimicrobial agents for agriculture are highly toxic and nonbiodegradable and thus cause extended environmental pollution. Moreover, an increasing number of phytopathogens have developed resistance to antimicrobial agents. The lipopeptides have been tried as potent versatile weapons to deal with a variety of phytopathogens. All the three families of Bacillus lipopeptides, namely, Surfactins, Iturins and Fengycins, have been explored for their antagonistic activities towards a wide range of phytopathogens including bacteria, fungi, and oomycetes. Iturin and Fengycin have antifungal activities, while Surfactin has broad range of potent antibacterial activities and this has also been used as larvicidal agent. Interestingly, lipopeptides being the molecules of biological origin are environmentally acceptable.

show abstract

Section: Introductionmentioning

confidence: 99%

Lipopeptides as the Antifungal and Antibacterial Agents: Applications in Food Safety and Therapeutics

Meena

Kanwar

2015

BioMed Research International

340

203

View full text Add to dashboard Cite

show abstract

“…Peptides with anticancer activities have been studied to develop "peptide vaccines." Currently, several peptide vaccines are available for breast cancer treatment as well as for many other types of cancer [1]. For example, peptides with anti-breast cancer activities, such as GP2, E75 and AE37, were successfully introduced into clinical trials [5,6,16].…”

Section: Discussionmentioning

confidence: 99%

“…In previous decades, numerous attempts have been made to develop therapeutic peptide vaccines for cancer [1]. Several peptide vaccines have been developed to induce anti-tumor immune responses in breast cancer patients [2].…”

Section: Introductionmentioning

confidence: 99%

The Effect of Peptide Treatment on the HLA-Binding and Antibody Production in Peripheral Blood Mononuclear Cells Obtained from Japanese Breast Cancer Patients

Kametani¹

2012

J Vaccines Vaccin

View full text Add to dashboard Cite

Background: Our previous predictive peptide binding studies indicated that a novel 20-mer multiple antigen peptide, CH401MAP, containing an anti-human epidermal growth factor receptor 2 (HER2) monoclonal antibody epitope (N163-182), may potentially bind to more than 95% of class I human leukocyte antigens (HLAs) and to 30-50% of class II HLAs expressed on peripheral blood mononuclear cells (PBMCs). In this study, CH401MAP was used for in vitro stimulation of PBMCs obtained from Japanese breast cancer patients, and anti-CH401MAP antibody secretion was evaluated. Methods: PBMCs of breast cancer patients were stimulated with CH401MAP peptide in vitro. Eight days after stimulation, the culture supernatants were collected and the anti-CH401MAP antibody levels were determined using enzyme-linked immunosorbent assay. The correlation of the antibody level and HER2 expression level after in vitro stimulation was also evaluated. Results: CH401MAP specific antibody was detected in the culture supernatants of patients' PBMCs after in vitro culture, irrespective of the peptide stimulation. The antibody levels of the three patient's groups were significantly higher than that of HD group. Significant correlation was not observed between specific antibody production and cancer progression. Conclusion: The PBMC of Japanese breast cancer patients possessed the potential of anti-CH401MAP antibody secretion. The antibody secretion level was significantly higher than that of HD. It is correlated with the expression of HER2 on the cancer tissues but not with the HER2 level in the sera of patients.

show abstract

“…Expression host may be an important factor in LPBV production as fatty acid incorporation in vivo not only varies among species but also results in a mixture of different lipoprotein structures that may have varying immunomodulating properties (86). This principle, however, has only been demonstrated when comparing the immunogenicity of naturally and synthetically produced LPBVs (87).…”

Section: Genetic Fusion Of Pamp To Pbvmentioning

confidence: 99%

Designs of Antigen Structure and Composition for Improved Protein-Based Vaccine Efficacy

et al. 2020

View full text Add to dashboard Cite

Today, vaccinologists have come to understand that the hallmark of any protective immune response is the antigen. However, it is not the whole antigen that dictates the immune response, but rather the various parts comprising the whole that are capable of influencing immunogenicity. Protein-based antigens hold particular importance within this structural approach to understanding immunity because, though different molecules can serve as antigens, only proteins are capable of inducing both cellular and humoral immunity. This fact, coupled with the versatility and customizability of proteins when considering vaccine design applications, makes protein-based vaccines (PBVs) one of today's most promising technologies for artificially inducing immunity. In this review, we follow the development of PBV technologies through time and discuss the antigen-specific receptors that are most critical to any immune response: pattern recognition receptors, B cell receptors, and T cell receptors. Knowledge of these receptors and their ligands has become exceptionally valuable in the field of vaccinology, where today it is possible to make drastic modifications to PBV structure, from primary to quaternary, in order to promote recognition of target epitopes, potentiate vaccine immunogenicity, and prevent antigen-associated complications. Additionally, these modifications have made it possible to control immune responses by modulating stability and targeting PBV to key immune cells. Consequently, careful consideration should be given to protein structure when designing PBVs in the future in order to potentiate PBV efficacy.

show abstract

A Purified Recombinant Lipopeptide as Adjuvant for Cancer Immunotherapy

Cited by 7 publications

References 37 publications

Lipopeptides as the Antifungal and Antibacterial Agents: Applications in Food Safety and Therapeutics

Lipopeptides as the Antifungal and Antibacterial Agents: Applications in Food Safety and Therapeutics

The Effect of Peptide Treatment on the HLA-Binding and Antibody Production in Peripheral Blood Mononuclear Cells Obtained from Japanese Breast Cancer Patients

Designs of Antigen Structure and Composition for Improved Protein-Based Vaccine Efficacy

Contact Info

Product

Resources

About