A purine scaffold Hsp90 inhibitor destabilizes BCL-6 and has specific antitumor activity in BCL-6–dependent B cell lymphomas

Cerchietti, Leandro; Lopes, Eloisi Caldas; Yang, Shao Ning; Hatzi, Katerina; Bunting, Karen; Tsikitas, Lucas; Mallik, Alka; Robles, Ana I.; Walling, Jennifer; Varticovski, Lyuba; Shaknovich, Rita; Bhalla, Kapil N.; Chiosis, Gabriela; Melnick, Ari

doi:10.1038/nm.2059

Cited by 149 publications

(165 citation statements)

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“…HSP90 is a molecular chaperone that plays a key role in the conformational maturation of oncogenic signalling proteins, including HER2/ERBB2, AKT, RAF1, BCR-ABL, and mutated p53. Tumour cells could contain HSP90 complexes in an activated, high-affinity conformation that facilitates malignant progression, which may represent a unique target for cancer therapeutics [15,16]. The transcription factor SP1 is a DNA-binding protein that interacts with a variety of gene promoters containing GC-box elements.…”

Section: Identification Of Hif1-α Interacting Proteins With Bioinformmentioning

confidence: 99%

Searching for Hif1-α interacting proteins in renal cell carcinoma

et al. 2012

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Introduction. Kidney tumours are frequently characterised by hypoxic conditions due to a local imbalance between oxygen (O 2 ) supply and consumption. Hif1-α regulates angiogenesis, tumour growth, tumour progression, metastatic spread, and glucose metabolism by acting as a transcription factor for relevant genes. Here, we describe an immunohistochemical study of Hif1-α, a comprehensive computational study of Hif1-α interacting proteins (HIPs), an analysis correlating expression levels of Hif1-α with upstream and downstream proteins, and an analysis of the utility of Hif1-α for prognosis in a cohort of patients with renal cell carcinoma. Materials and methods. The patient cohort included 80 patients. For immunohistochemistry evaluation, tissue microarrays were constructed. The IntAct, MINT, and BOND databases were used for the HIP approach. The Kruskal-Wallis test was used for comparing protein expression with pathology measurements. Correlation was expressed as the Pearson coefficient. Results. Hif1-α expression correlates significantly with the "clear" histological subtype of renal cell carcinoma (p < 0.01). The samples with the worst prognoses related to the pathological variables analysed showed the highest levels of Hif1-α expression. Significant correlations were found with Bcl-2, CAIX, C-kit, EGFR, TGF-β, proteins of the VEGF family, proteins related to differentiation (such as Notch1 and Notch3) and certain metabolic enzymes. Bioinformatic analysis suggested 45 evidence-based HIPs and 4 complexes involving protein Hif1-α. Conclusions. This work summarises the multifaceted role of Hif1-α in the pathology of renal cell carcinomas, and it identifies HIPs that could help provide mechanistic explanations for the different behaviours seen in tumours.

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Section: Identification Of Hif1-α Interacting Proteins With Bioinformmentioning

confidence: 99%

Searching for Hif1-α interacting proteins in renal cell carcinoma

et al. 2012

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“…The hsp90 inhibitor PU-H71 has demonstrated preclinical activity in bcl-6 -dependent large-cell lymphomas, making it potentially relevant for clinical development in B-cell malignancies derived from the germinal center, including FL. 53 …”

Section: Bcl-6 Inhibitorsmentioning

confidence: 99%