A putative HCO−3 transporter in the cyanobacterium Synechococcus sp. strain PCC 79421

Bonfil, David J.; Ronen-Tarazi, Michal; Sültemeyer, Dieter; Lieman-Hurwitz, Judy; Schatz, Daniella

doi:10.1016/s0014-5793(98)00662-0

Cited by 63 publications

(44 citation statements)

References 28 publications

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“…This concentration process involves the action of active transporters thought to be associated with the cytoplasmic membrane. The predicted energy-dependent intracellular accumulation of HCO 3 Ϫ has been experimentally demonstrated in a number of cyanobacteria and in H. neapolitanus (10,29,35,42). Moreover, ectopic expression of human CA in the cytoplasm of Synechococcus strain PCC7942 yields transformants that have the high-CO 2 -requiring (HCR) phenotype and leak CO 2 into the surrounding medium (47).…”

Section: Carboxysome Functionmentioning

confidence: 87%

“…Despite the attractiveness of the hypothesis, CA does not appear to colocalize with carboxysomes from several chemoautotrophs and cyanobacteria (6,13,18,22,38). Nevertheless, several groups have reported CA activity in cyanobacterial carboxysome preparations and have taken this as evidence for the role of the particles in an inorganic carbon-concentrating mechanism (CCM) in those organisms (2,10,34,49,66). Carboxysomes are thought to represent the second stage of the CCM that rapidly converts HCO 3 Ϫ to CO 2 to feed the RuBisCO-catalyzed carboxylation reaction.…”

Section: Carboxysome Functionmentioning

confidence: 99%

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Microcompartments in Prokaryotes: Carboxysomes and Related Polyhedra

Cannon

Bradburne

Aldrich

et al. 2001

Appl Environ Microbiol

210

201

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Section: Carboxysome Functionmentioning

confidence: 87%

Section: Carboxysome Functionmentioning

confidence: 99%

Microcompartments in Prokaryotes: Carboxysomes and Related Polyhedra

Cannon

Bradburne

Aldrich

et al. 2001

Appl Environ Microbiol

210

201

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“…P. marinus strains MED4 and PCC 9511 are therefore naturally deficient for assimilation of these nitrogen sources. In contrast, the ure genes encoding the urease complex that have been Shi et al (1998) ; 2, Leonard et al (1998) ;3, Irmler & Forchhammer (2001) ; 4, Shibata et al (2001) ; 5, Bonfil et al (1998) ;6, Omata et al (2001). characterized in strain PCC 9511 (Palinska et al, 2000) and amt1, one of the three ORFs encoding ammonium permeases in Synechocystis sp.…”

Section: Lack Of Nitrate and Nitrite Assimilationmentioning

confidence: 99%

The signal transducer PII and bicarbonate acquisition in Prochlorococcus marinus PCC 9511, a marine cyanobacterium naturally deficient in nitrate and nitrite assimilation a aThe GenBank accession number for the glnB gene sequence reported in this paper is AJ271089.

et al. 2002

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The amino acid sequence of the signal transducer P II (GlnB) of the oceanic photosynthetic prokaryote Prochlorococcus marinus strain PCC 9511 displays a typical cyanobacterial signature and is phylogenetically related to all known cyanobacterial glnB genes, but forms a distinct subclade with two other marine cyanobacteria. P II of P. marinus was not phosphorylated under the conditions tested, despite its highly conserved primary amino acid sequence, including the seryl residue at position 49, the site for the phosphorylation of the protein in the cyanobacterium Synechococcus PCC 7942. Moreover, P. marinus lacks nitrate and nitrite reductase activities and does not take up nitrate and nitrite. This strain, however, expresses a low-and a high-affinity transport system for inorganic carbon (C i ; K m,app 240 and 4 µM, respectively), a result consistent with the unphosphorylated form of P II acting as a sensor for the control of C i acquisition, as proposed for the cyanobacterium Synechocystis PCC 6803. The present data are discussed in relation to the genetic information provided by the P. marinus MED4 genome sequence.

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“…Today, it is widely accepted that the low CO 2 affinity of Rubisco is compensated by an efficient inorganic carbon (C i )-concentrating mechanism (CCM) that raises the concentration of CO 2 in close proximity to Rubisco (13)(14)(15). Mutants impaired in functional components of the CCM, such as the carboxysomes (16)(17)(18)(19) or transport and internal accumulation of C i (20)(21)(22), show very low apparent photosynthetic affinity for external C i and, thus, exhibit a HCR phenotype. These findings clearly revealed the essential function of the CCM for cyanobacterial survival under the present atmosphere and prompted the widely accepted notion that oxygenase activity of Rubisco is almost totally repressed in cyanobacteria.…”

mentioning

confidence: 99%

The photorespiratory glycolate metabolism is essential for cyanobacteria and might have been conveyed endosymbiontically to plants

Eisenhut

Ruth

Haimovich

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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274

308

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Photorespiratory 2-phosphoglycolate (2PG) metabolism is essential for photosynthesis in higher plants but thought to be superfluous in cyanobacteria because of their ability to concentrate CO 2 internally and thereby inhibit photorespiration. Here, we show that 3 routes for 2PG metabolism are present in the model cyanobacterium Synechocystis sp. strain PCC 6803. In addition to the photorespiratory C2 cycle characterized in plants, this cyanobacterium also possesses the bacterial glycerate pathway and is able to completely decarboxylate glyoxylate via oxalate. A triple mutant with defects in all 3 routes of 2PG metabolism exhibited a high-CO 2-requiring (HCR) phenotype. All these catabolic routes start with glyoxylate, which can be synthesized by 2 different forms of glycolate dehydrogenase (GlcD). Mutants defective in one or both GlcD proteins accumulated glycolate under high CO 2 level and the double mutant ⌬glcD1/⌬glcD2 was unable to grow under low CO2. The HCR phenotype of both the double and the triple mutant could not be attributed to a significantly reduced affinity to CO2, such as in other cyanobacterial HCR mutants defective in the CO2-concentrating mechanism (CCM). These unexpected findings of an HCR phenotype in the presence of an active CCM indicate that 2PG metabolism is essential for the viability of all organisms that perform oxygenic photosynthesis, including cyanobacteria and C3 plants, at ambient CO 2 conditions. These data and phylogenetic analyses suggest cyanobacteria as the evolutionary origin not only of oxygenic photosynthesis but also of an ancient photorespiratory 2PG metabolism. I t is well established that the photorespiratory C2 pathway, whereby 2-phosphoglycolate (2PG) is metabolized (1), is essential for photosynthesis in the majority of plants (2). In contrast, the functioning of the C2 pathway and its importance are still under discussion for cyanobacteria. These organisms were the first to have evolved oxygenic photosynthesis, and endosymbiotic engulfment of an ancient cyanobacterium led to the evolution of plant chloroplasts (3). In cyanobacteria, as in C3 plants, the primary carbon fixation is catalyzed by ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco). Ribulose 1,5-bisphosphate reacts with either CO 2 , leading to the formation of 2 molecules of 3-phosphoglycerate (3PGA), or O 2 , generating 3PGA and 2PG. The latter compound is toxic to plant metabolism because it inhibits distinct steps in the carbon-fixing Calvin-Benson cycle (4, 5). Therefore, plants employ the socalled photorespiratory glycolate pathway (or C2 cycle), which degrades 2PG and converts 2 molecules of 2PG into 1 molecule each of 3PGA, CO 2 , and NH 4 ϩ (1, 6, 7). In a typical C3 plant, the ammonium is refixed at the expense of ATP, and 25% of the carbon entering the path is released as CO 2 . Generally, the photorespiratory cycle is indispensable for C3 plants, because mutations in single steps of the C2 cycle resulted in high-CO 2 -requiring (HCR) phenotypes (2,(8)(9)(10).In contrast to plants, ear...

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A putative HCO⁻₃ transporter in the cyanobacterium Synechococcus sp. strain PCC 7942¹

Cited by 63 publications

References 28 publications

Microcompartments in Prokaryotes: Carboxysomes and Related Polyhedra

Microcompartments in Prokaryotes: Carboxysomes and Related Polyhedra

The signal transducer PII and bicarbonate acquisition in Prochlorococcus marinus PCC 9511, a marine cyanobacterium naturally deficient in nitrate and nitrite assimilation a aThe GenBank accession number for the glnB gene sequence reported in this paper is AJ271089.

The photorespiratory glycolate metabolism is essential for cyanobacteria and might have been conveyed endosymbiontically to plants

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