2022
DOI: 10.3389/fpls.2022.852500
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A Putative P-Type ATPase Regulates the Secretion of Hydrolytic Enzymes, Phospholipid Transport, Morphogenesis, and Pathogenesis in Phytophthora capsici

Abstract: Phytophthora capsici is an important plant pathogenic oomycete with multiple hosts. The P4-ATPases, aminophospholipid translocases (APTs), play essential roles in the growth and pathogenesis of fungal pathogens. However, the function of P4-ATPase in P. capsici remains unclear. This study identified and characterized PcApt1, a P4-ATPase Drs2 homolog, in P. capsici. Deletion of PcAPT1 by CRISPR/Cas9 knock-out strategy impaired hyphal growth, extracellular laccase activity. Cytological analyses have shown that Pc… Show more

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Cited by 4 publications
(2 citation statements)
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“…capsici is one of the most destructive pathogens affecting pepper plants, causing significant losses in pepper quality and yields. 52 The antifungal performances of COS and its derivatives against Ph. capsici are shown in Figure 8d.…”
Section: Antifungal Activity (In Vitro)mentioning
confidence: 99%
“…capsici is one of the most destructive pathogens affecting pepper plants, causing significant losses in pepper quality and yields. 52 The antifungal performances of COS and its derivatives against Ph. capsici are shown in Figure 8d.…”
Section: Antifungal Activity (In Vitro)mentioning
confidence: 99%
“…29 Homologous flanking sequences containing ∼1kb upstream and downstream of the site were amplified and linked to the linearized pBluescript II KS + to obtain gene replacement plasmid constructs. The Polyethylene glycol-mediated protoplast transformation was carried out according to the previously described technique, 29,30 with a minor modification. An all-in-one plasmid and the donor plasmid were cotransformed into protoplasts of P. capsici isolate LT1534.…”
Section: Verification Of Point Mutation Transformants By Crispr/cas9mentioning
confidence: 99%