A putative protein structurally related to zygote arrest 1 (Zar1), Zar1-like, is encoded by a novel gene conserved in the vertebrate lineage

Sangiorgio, Lorenzo; Strumbo, Bice; Brevini, Tiziana A. L.; Ronchi, Severino; Simonic, Tatjana

doi:10.1016/j.cbpb.2008.03.007

Cited by 17 publications

(32 citation statements)

References 33 publications

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“…However, they exhibit a high similarity in protein sequence in their C-terminus. When our Zar1l project was ongoing, Sangiorgio et al (2008) reported the expression and preliminary sequence analysis of Bovine ZAR1-like gene and its orthologs in vertebrates. Unlike bovine ZAR1-like gene, we found that mouse Zar1-like gene was predominantly expressed in ovaries, oocytes, and early preimplantation embryos.…”

Section: Wu Et Al (2003a) Have Reported Thatmentioning

confidence: 99%

Mouse ZAR1‐like (XM_359149) colocalizes with mRNA processing components and its dominant‐negative mutant caused two‐cell‐stage embryonic arrest

Wang

Zhu

et al. 2010

Developmental Dynamics

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*Maternal effect genes and encoding proteins are necessary for nuclear reprogramming and zygotic genome activation. However, the mechanisms that mediate these functions are largely unknown. Here we identified XM_359149, a Zar1-like gene that is predominantly expressed in oocytes and zygotes, which we designated Zar1-like (Zar1l). ZAR1L-EGFP formed multiple cytoplasmic foci in late two-cell-stage embryos. Expression of the ZAR1L C-terminus induced two-cell-stage embryonic arrest, accompanied with abnormal methylation of histone H3K4me2/3 and H3K9me2/3, and marked down-regulation of a group of chromatin modification factors including Dppa2, Dppa4, and Piwil2. When ectopically expressed in somatic cells, ZAR1L colocalized with P-body components including EIF2C1(AGO1), EIF2C2(AGO2), DDX6 and LSM14A, and germline-specific chromatoid body components including PIWIL1, PIWIL2, and LIN28. ZAR1L colocalized with ZAR1 and interacted with human LIN28. Our data suggest that ZAR1L and ZAR1 may comprise a novel family of processing-body/chromatoid-body components that potentially function as RNA regulators in early embryos.

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Section: Wu Et Al (2003a) Have Reported Thatmentioning

confidence: 99%

Mouse ZAR1‐like (XM_359149) colocalizes with mRNA processing components and its dominant‐negative mutant caused two‐cell‐stage embryonic arrest

Wang

Zhu

et al. 2010

Developmental Dynamics

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“…Amino acid sequence comparison revealed the presence of a FYVE/PHD zinc finger (ZnF) domain, as indicated by the presence of the conserved 12 cysteine pattern in the C-terminus of the deduced chicken Zar1 and Zar1-like proteins, which was also conserved in the C-terminus of mouse and human proteins. This ZnF domain, which is conserved from yeast to human, was also observed in the C-terminal region of other vertebrate Zar1 and Zar1-like proteins, including human, mice, rat, xenopus, zebrafish, fugu, cattle, pig and dog (Uzbekova et al 2006;Sangiorgio et al 2008). Although typical PHD motif is a conserved C 8 pattern, the atypical PHD motif in all Zar1 and Zar1-like orthologs is a conserved C 12 pattern.…”

Section: Discussionmentioning

confidence: 94%

“…In addition the sequences for mouse and human Zar1 and Zar1-like genes, reported by Sangiorgio et al (2008), were also included in the sequence comparison in order to identify sequence similarities of these proteins in different vertebrates.…”

Section: Protein Sequence Comparisonmentioning

confidence: 99%

“…Primers for reverse transcriptase polymerase chain reaction (RT-PCR) were designed based on the chicken for Zar1 and Zar1-like cDNA sequences reported by Sangiorgio et al (2008). The primer sequences for the Zar1 gene were forward 5′-CAC TGC AAG GAC TGC AAC AT-3 and reverse 5′-CTC TGG CAG GTG ATG TCC TC-3′ and for the Zar1-like gene were forward 5′-TCT CCG GGA GTA ACA AGG TG-3′ and reverse 5′-GAG GGC ACA CAA GTC AGA CA-3′.…”

Section: Rna Isolation and Rt-pcr Analysismentioning

confidence: 99%

“…More recently it has been reported that a chicken Zar1 transcript was identified and it's expression was observed in the ovary, in oocytes during follicular maturation and in early embryo development and was expressed at higher levels after activation of the chicken embryonic genome (Elis et al 2008). Furthermore, Sangiorgio et al (2008) using in silico analyses identified the sequences of genes encoding a novel protein structurally related to Zar1, called Zar1-like in cattle and many other vertebrate species, including the chicken.…”

Section: Introductionmentioning

confidence: 96%

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Expression of chicken zygote arrest 1 (Zar1) and Zar1-like genes during sexual maturation and embryogenesis

2010

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Maternal mRNAs, which are expressed in oocytes, play an important role in the success of early embryo development, as they allow the first cleavages to occur. Zygote arrest 1 (Zar1) is an oocyte-specific maternal-effect gene that functions at the oocyte-toembryo transition in many vertebrate species including human, pig, cattle, sheep, mouse, rat, frog and zebrafish. Recently, through in silico studies, a gene structurally related to Zar1, called Zar1-like has been identified in many vertebrates, including the chicken. The objectives of this study were to investigate the expression of the chicken Zar1 and Zar1-like genes in chicken tissues and embryos and to determine whether sexual maturation affects their mRNA abundance. RNA was extracted from various organs of chickens aged from one month up to two years old and from chicken embryos until day ten of embryonic development. Expression analysis of the genes was performed using RT-PCR and real-time PCR. RT-PCR analysis revealed that both genes were preferentially expressed in chicken oocytes, ovary and testes and in embryos during embryonic development. Quantitative realtime PCR analysis revealed a significant up regulation of Zar1 in the mature ovary, and also a significant up regulation of Zar1 and Zar1-like genes in the testes of sexually mature roosters, suggesting a key role of these genes in the chicken fertility. In contrast, expression of Zar1-like was not affected by age in the chicken ovary. Our results indicate that the chicken Zar1 and Zar1-like transcripts are co-expressed in high levels in the chicken gonads. In addition their expression beyond the stage of embryonic genome activation suggests an embryonic and not only a maternal origin of these transcripts.

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Revisiting ZAR proteins: the understudied regulator of female fertility and beyond

Fan

2022

Cell. Mol. Life Sci.

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A putative protein structurally related to zygote arrest 1 (Zar1), Zar1-like, is encoded by a novel gene conserved in the vertebrate lineage

Cited by 17 publications

References 33 publications

Mouse ZAR1‐like (XM_359149) colocalizes with mRNA processing components and its dominant‐negative mutant caused two‐cell‐stage embryonic arrest

Mouse ZAR1‐like (XM_359149) colocalizes with mRNA processing components and its dominant‐negative mutant caused two‐cell‐stage embryonic arrest

Expression of chicken zygote arrest 1 (Zar1) and Zar1-like genes during sexual maturation and embryogenesis

Revisiting ZAR proteins: the understudied regulator of female fertility and beyond

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