A Pyramid Breeding of Eight Grain-yield Related Quantitative Trait Loci Based on Marker-assistant and Phenotype Selection in Rice (Oryza sativa L.)

Zong, Guo; Wang, Ahong; Wang, Lu; Liang, Guohua; Gu, Minghong; Su, Tao; Han, Bin

doi:10.1016/j.jgg.2012.06.004

Cited by 35 publications

(15 citation statements)

References 38 publications

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“…Hence, molecular breeding in rice aids to pyramid elite allele of important genes of yield, quality and resistance into elite cultivars (Chen et al ., ; Wang et al ., ; Zhou et al ., ); therefore, assessment of allele performance is the prerequisite and biggest task. Recently, assessment of allele performance mainly depend on QTL analysis with genetic populations such as NILs and CSSLs (Ohsumi et al ., ; Zong et al ., ), which are time and money consuming. In our study, Huanghuazhan was bred after several decades of artificial selection, having conserved amount of valuable genome segments which was called HTBs in the study.…”

Section: Discussionmentioning

confidence: 99%

Pedigree‐based analysis of derivation of genome segments of an elite rice reveals key regions during its breeding

Zhou

Chen

Lin

et al. 2015

Plant Biotechnology Journal

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SummaryAnalyses of genome variations with high-throughput assays have improved our understanding of genetic basis of crop domestication and identified the selected genome regions, but little is known about that of modern breeding, which has limited the usefulness of massive elite cultivars in further breeding. Here we deploy pedigree-based analysis of an elite rice, Huanghuazhan, to exploit key genome regions during its breeding. The cultivars in the pedigree were resequenced with 7.69 depth on average, and 2.1 million high-quality single nucleotide polymorphisms (SNPs) were obtained. Tracing the derivation of genome blocks with pedigree and information on SNPs revealed the chromosomal recombination during breeding, which showed that 26.22% of Huanghuazhan genome are strictly conserved key regions. These major effect regions were further supported by a QTL mapping of 260 recombinant inbred lines derived from the cross of Huanghuazhan and a very dissimilar cultivar, Shuanggui 36, and by the genome profile of eight cultivars and 36 elite lines derived from Huanghuazhan. Hitting these regions with the cloned genes revealed they include numbers of key genes, which were then applied to demonstrate how Huanghuazhan were bred after 30 years of effort and to dissect the deficiency of artificial selection. We concluded the regions are helpful to the further breeding based on this pedigree and performing breeding by design. Our study provides genetic dissection of modern rice breeding and sheds new light on how to perform genomewide breeding by design.

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Section: Discussionmentioning

confidence: 99%

Pedigree‐based analysis of derivation of genome segments of an elite rice reveals key regions during its breeding

Zhou

Chen

Lin

et al. 2015

Plant Biotechnology Journal

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“…Exotic genetic libraries are a useful resource for crop improvement ( Zamir, 2001 ). Identification and pyramiding of yield-related QTLs were performed in rice ( Ashikari and Matsuoka, 2006 ; Zong et al, 2012 ) and tomato ( Gur and Zamir, 2004 ). In the current study, we demonstrated a start-to-end pathway that uses an exotic nearly isogenic library to address yield improvement in tomato followed by thorough genetic dissection of epistasis between the three underlying pyramided loci.…”

Section: Discussionmentioning

confidence: 99%

Mendelizing all Components of a Pyramid of Three Yield QTL in Tomato

Gur

Zamir

2015

Front. Plant Sci.

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Molecular markers allowed breeders to mendelize quantitative trait loci (QTL) providing another demonstration that quantitative traits are governed by the same principles as single qualitative genes. This research extends the QTL analysis to two and three QTL and tests our ability to mendelize an oligogenic trait. In tomato, agricultural yield is determined by the weight of the fruits harvested per unit area and the total soluble solids (% Brix)–sugars and acids. The current study explores the segregation of multiple independent yield-related QTL that were identified and mapped using introgression lines (IL) of Solanum pennellii in cultivated processing tomato (S. lycopersicum). We screened 45 different double and triple IL-QTL combinations for agricultural yield, to identify QTL pyramids that behaved in an additive manner and were suitable substrate for mendelizing an oligogenic trait. A pyramid of three independent QTL that significantly improved Brix∗Yield (BXY - the soluble solids output per unit area) compared to M82 was selected. In the progenies of the tri-hybrid we bred using markers a nearly isogenic ‘immortalized F2.’ While the common mode of QTL–QTL interactions across the 45 IL-QTLs combinations was less than additive, the three QTLs in the selected triple-stack performed in an additive manner which made it an exceptional material for breeding. This study demonstrates that using the phenotypic effect of all 27 possible QTL-alleles combinations it is possible to make reliable predictions about the genotypes that will maximize the yield.

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“…Recently, the characteristics of panicle size have been improved significantly by clustering 8 panicle number and weight-related QTLs via MAS (Zong et al 2012). Meanwhile, the strong restorer line Q611 and the hybrid rice variety Y-You-7 were obtained by transferring two high-yield genes of the Malaysian wild rice Oryza rufipogon , yld1.1 and yld2.1 (Wu et al 2010a), into the elite restorer lines Ce64-7 and 9311 via MAS (Table 1).…”

Section: Molecular Breeding For High Yieldmentioning

confidence: 99%

Recent progress on molecular breeding of rice in China

Rao

Qian

2014

Plant Cell Rep

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Molecular breeding of rice for high yield, superior grain quality, and strong environmental adaptability is crucial for feeding the world’s rapidly growing population. The increasingly cloned quantitative trait loci and genes, genome variations, and haplotype blocks related to agronomically important traits in rice have provided a solid foundation for direct selection and molecular breeding, and a number of genes have been successfully introgressed into mega varieties of rice. Here we summarize China’s great achievements in molecular breeding of rice in the following five traits: high yield, biotic stress resistance, abiotic stress resistance, quality and physiology. Further, the prospect of rice breeding by molecular design is discussed.

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A Pyramid Breeding of Eight Grain-yield Related Quantitative Trait Loci Based on Marker-assistant and Phenotype Selection in Rice (Oryza sativa L.)

Cited by 35 publications

References 38 publications

Pedigree‐based analysis of derivation of genome segments of an elite rice reveals key regions during its breeding

Pedigree‐based analysis of derivation of genome segments of an elite rice reveals key regions during its breeding

Mendelizing all Components of a Pyramid of Three Yield QTL in Tomato

Recent progress on molecular breeding of rice in China

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