A pyrolysis-mass spectrometry study of Corynebacterium spp.

Hindmarch, J. M.; Magee, J. T.; Hadfield, M. A.; Duerden, B. I.

doi:10.1099/00222615-31-2-137

Cited by 36 publications

(8 citation statements)

References 28 publications

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“…However, the interstrain similarity between them (40 %), was less than that for all strains of CD2 suggesting that CD2 is a more homogeneous taxon than C. jeikeium. This finding is supported by Hindmarch and co-workers [22] in a pyrolysis-mass spectrometric analysis of Corynebacterium spp., CD2 strains could be accommodated in a single cluster whereas C. jeikeium strains were located in six clusters.…”

Section: Di)is( Lssl( )Nsupporting

confidence: 70%

A numerical analysis of ribosomal RNA gene patterns for typing clinical isolates ofCorynebacteriumgroup D2

Soto

Pitcher²,

Soriano

1991

Epidemiol. Infect.

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SUMMARYRestriction digest fragments of DNA from 46 clinical isolates identified as Corynebacterium group D2, were separated by electrophoresis, Southern blotted onto nylon membranes and hybridized to a ribosomal RNA gene probe. The resulting band patterns were subjected to unweighed pair-group cluster analysis. Representative strains from the main clusters were compared with similarly prepared band patterns from type strains of human Corynebacterium species.The results indicate that strains identified as Corynebacterium group D2 represent a unique taxon and that computer-assisted analysis of rRNA gene restriction fragment polymorphism (ribotyping) could be a useful technique in epidemiological studies of these bacteria.

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Section: Di)is( Lssl( )Nsupporting

confidence: 70%

A numerical analysis of ribosomal RNA gene patterns for typing clinical isolates ofCorynebacteriumgroup D2

Soto

Pitcher²,

Soriano

1991

Epidemiol. Infect.

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“…For identifying the coryneforms, these systems were reasonably accurate (5,6,9,15,17), but for identifying anaerobes, there were significant gaps, mainly with the biochemically inactive clostridia (3,13,16). For the identification of other anaerobic genera, results have been variable.…”

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confidence: 99%

Multicenter Evaluation of the Vitek 2 Anaerobe and Corynebacterium Identification Card

et al. 2008

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The new anaerobe and Corynebacterium (ANC) identification card for Vitek 2 was compared with a 16S rRNA gene sequencing (16S) reference method for accuracy in the identification of corynebacteria and anaerobic species. Testing was performed on a Vitek 2 XL system with modified software at three clinical trial laboratories. Reproducibility was determined with nine ATCC quality control strains that were tested 20 times over a minimum of 10 days at all three sites. A challenge set of 50 well-characterized strains and 365 recent fresh and frozen clinical isolates were included in the study. The expected positive and negative biochemical well reactions were also evaluated for substrate reproducibility. All strains were tested with the ANC card, and clinical isolates were saved for 16S rRNA gene sequencing. All reproducibility tests yielded expected results within a 95% confidence interval, except for that with Corynebacterium striatum ATCC 6940, for which identification failed at one trial site. For the challenge isolates, there was 98% correct identification, 5% low discrimination, and 2% incorrect identification, and 0% were unidentified. For clinical strains, there was 95.1% correct identification, 4.9% low discrimination, and 4.6% incorrect identification, and 0.3% were unidentified. The 4.6% (17/365) of clinical isolates that were incorrectly identified consisted of 14 isolates that were correct at the genus level and three that were incorrect at the genus level. The new ANC card met all performance criteria within a 95% confidence interval compared to the identification performance by 16S rRNA gene sequencing.

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“…All agar was sterilized by autoclaving at 121 °C for 15 min before use. Isolated organisms were tentatively identified using the standard biochemical tests in the API 20NE test kits (bioMe´rieux) and further examined by pyrrolysis mass‐spectroscopy ( Hindmarch et al . 1990 ).…”

Section: Methodsmentioning

confidence: 99%

The degradation of sodiumO,O‐diethyl dithiophosphate by bacteria from metalworking fluids

Sherburn

Large

1999

Letters in Applied Microbiology

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The breakdown of sodium O,O‐diethyl dithiophosphate (O,O‐diethyl phosphorodithioate) by four bacterial strains (tentatively identified as strains of Aeromonas, Pseudomonas, Flavobacterium and Bacillus) isolated from contaminated metalworking fluids was shown to involve the successive formation of ethanol, aldehyde and orthophosphate. An acid phosphodiesterase was identified in cell‐free extracts that was five‐ to sevenfold enhanced in specific activity in bacteria grown on O,O‐diethyl dithiophosphate as sole phosphorus source, compared with bacteria grown on orthophosphate. This is thought to initiate the breakdown process.

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A pyrolysis-mass spectrometry study of Corynebacterium spp.

Cited by 36 publications

References 28 publications

A numerical analysis of ribosomal RNA gene patterns for typing clinical isolates ofCorynebacteriumgroup D2

A numerical analysis of ribosomal RNA gene patterns for typing clinical isolates ofCorynebacteriumgroup D2

Multicenter Evaluation of the Vitek 2 Anaerobe and Corynebacterium Identification Card

The degradation of sodiumO,O‐diethyl dithiophosphate by bacteria from metalworking fluids

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