Plate girders are widely used as structural members in the steel construction industry because of their ability to support heavy loads over long spans. Therefore, it is very important to know their behaviour under different conditions. For material efficiency, plate girders usually have slender webs when compared to the ones on commercial hot rolled profiles, making them prone to the occurrence of shear buckling. In case of fire, shear buckling may be precipitated due to the reduction of the steel mechanical properties caused by the elevated temperatures. Regarding the resistance determination, numerical simulation has been gaining an edge on experimental analysis over the past years, mainly due to the high cost of the fire resistance experimental tests. However, the numerical models must be properly validated in order to perform reliable numerical studies. With this purpose, as no experimental tests in stainless steel plate girders subjected to elevated temperatures were found, a total of 34 experimental tests at normal temperature have been numerically modelled. The tested stainless steel plate girders had different configurations, provided with transversal and longitudinal stiffeners. Comparative analyses between those experimental and numerical results have been done. Afterwards, the developed numerical model has been used to perform a sensitivity analysis on the influence of the initial geometric imperfections at both normal and elevated temperatures, considering different values for its amplitudes. The effect of the residual stresses has also been analysed at normal temperature and in case of fire. Finally, comparisons between the numerical results and the Eurocode 3 design procedures have been performed.