A quadratic isothermal amplification fluorescent biosensor without intermediate purification for ultrasensitive detection of circulating tumor DNA

Wu, Zhaojie; Zheng, Hongshan; Bian, Yongjun; Weng, Jian; Zeng, Ru; Sun, Liping

doi:10.1039/d4an00460d

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2024

DOI: 10.1039/d4an00460d

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A quadratic isothermal amplification fluorescent biosensor without intermediate purification for ultrasensitive detection of circulating tumor DNA

Zhaojie Wu,

Hongshan Zheng,

Yongjun Bian

et al.

Abstract: A quadratic amplification fluorescence biosensor constructed by combining nicking enzyme mediated amplification (NEMA) and catalytic hairpin assembly (CHA) reactions. After quadratic amplification, this biosensor achieved nearly 107-fold, enabling it to detect ctDNA with ultra-sensitivity.

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Emerging Combination of Hydrogel and Electrochemical Biosensors

Huang,

Zhou,

et al. 2024

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Electrochemical sensors are among the most promising technologies for biomarker research, with outstanding sensitivity, selectivity, and rapid response capabilities that make them important in medical diagnostics and prognosis. Recently, hydrogels have gained attention in the domain of electrochemical biosensors because of their superior biocompatibility, excellent adhesion, and ability to form conformal contact with diverse surfaces. These features provide distinct advantages, particularly in the advancement of wearable biosensors. This review examines the contemporary utilization of hydrogels in electrochemical sensing, explores strategies for optimization and prospective development trajectories, and highlights their distinctive advantages. The objective is to provide an exhaustive overview of the foundational principles of electrochemical sensing systems, analyze the compatibility of hydrogel properties with electrochemical methodologies, and propose potential healthcare applications to further illustrate their applicability. Despite significant advances in the development of hydrogel‐based electrochemical biosensors, challenges persist, such as improving material fatigue resistance, interfacial adhesion, and maintaining balanced water content across various environments. Overall, hydrogels have immense potential in flexible biosensors and provide exciting opportunities. However, resolving the current obstacles will necessitate additional research and development efforts.

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Emerging Combination of Hydrogel and Electrochemical Biosensors

Huang,

Zhou,

et al. 2024

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A quadratic isothermal amplification fluorescent biosensor without intermediate purification for ultrasensitive detection of circulating tumor DNA

Abstract: A quadratic amplification fluorescence biosensor constructed by combining nicking enzyme mediated amplification (NEMA) and catalytic hairpin assembly (CHA) reactions. After quadratic amplification, this biosensor achieved nearly 107-fold, enabling it to detect ctDNA with ultra-sensitivity.

Cited by 1 publication

References 35 publications

Emerging Combination of Hydrogel and Electrochemical Biosensors

Emerging Combination of Hydrogel and Electrochemical Biosensors

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