A quantification of goldfish behavior by an image processing system

Upregulation of anti-apoptotic factors in upper motor neurons after spinal cord injury in adult zebrafish

“…To quantify the restoration of motor function, behavioral analysis was performed with a slight modification of previously described method (Kato et al, 1996;Kato et al, 2004;Kaneda et al, 2008). In brief, a single zebrafish was placed in a water tank (370 mm × 240 mm, water depth = 80 mm) and its spontaneous movement was captured by two video cameras (EVI-1011; Sony, Tokyo, Japan) placed both on the top and at the side of the tank for 5 min at 30 frames/sec.…”

“…In brief, a single zebrafish was placed in a water tank (370 mm × 240 mm, water depth = 80 mm) and its spontaneous movement was captured by two video cameras (EVI-1011; Sony, Tokyo, Japan) placed both on the top and at the side of the tank for 5 min at 30 frames/sec. The position of the fish in each frame was determined, and the three-dimensional coordination of the fish was then reconstructed by combining two images (from the top and side view) with previously developed software (Kato et al, 1996;Kato et al, 2004;Kaneda et al, 2008) on a computer (Precision 490; Dell, Round Rock, TX, USA). The total distance of swimming was calculated as described in Kato et al (1996).…”

“…The position of the fish in each frame was determined, and the three-dimensional coordination of the fish was then reconstructed by combining two images (from the top and side view) with previously developed software (Kato et al, 1996;Kato et al, 2004;Kaneda et al, 2008) on a computer (Precision 490; Dell, Round Rock, TX, USA). The total distance of swimming was calculated as described in Kato et al (1996). At least 3 fish were 11 recruited for each time point after SCI.…”

“…To evaluate the recovery of motor function in zebrafish after SCI, we measured moving behavior in a three-dimensional fashion with a computer image processing system (Kato et al, 1996;Kato et al, 2004;Kaneda et al, 2008). The unlesioned fish can swim freely in an aquarium without any limitation (Fig.…”

Comparing the EthoVision 2.3 system and a new computerized multitracking prototype system to measure the swimming behavior in fry fish

“…Thanks to the development of digital imaging techniques, a third approach in the laboratory is able to detect and measure behavioral parameters-impossible to quantify with manual recording-with precision and over long time periods (Kato, Tamada, Shimada, & Chujo, 1996;Martin, Prescott, & Zhu, 1992;Mukhina, Bachurin, Lermontova, & Zefirov, 2001;Nilsson, Rosén, & Johansson, 1993;Olivo & Thompson, 1988;Winberg, Nilsson, Spruijt, & Höglund, 1993). For example, the EthoVision system, a commercial (Noldus Information Technology) video tracking device, was developed to measure three elements: behaviors that occur briefly and are then interspersed with long periods of inaction, behaviors that occur over many hours, and spatial measurements (e.g., distance moved, moving velocity, radial speed, or time spent in different zones of an arena) , 2002Spink, Tegelenbosch, Buma, & Noldus, 2001).…”