A quantitative analysis of carbon-ion beam-induced reactive oxygen species and redox reactions

Matsumoto, Ken‐ichiro; Nyui, Minako; Ueno, Munetaka; Ogawa, Y.; Nakanishi, Ikuo

doi:10.3164/jcbn.18-34

Cited by 20 publications

(30 citation statements)

References 22 publications

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“…(25) High LET beams can create H 2 O 2 independently of oxygen, but low LET radiation creates H 2 O 2 mainly in an oxygendependent manner. (26) Therefore, high LET beams can yield H 2 O 2 Table 1. Gene specific primers used for quantitative RT PCR…”

Section: Resultsmentioning

confidence: 99%

“…X-ray irradiation was reported to cause only 0.26 mM/Gy of H 2 O 2 in water. (26) As H 2 O 2 generation caused by low LET radiation, such as X-rays, requires oxygen consumption, the X-ray-induced H 2 O 2 generation in cells or in vivo tissue may be much lower. Therefore, the main reactive oxygen species (ROS) causing oxidative stress during the X-ray irradiation in the thymocytes in this experiment was probably hydroxyl radical ( • OH).…”

Section: Gsh-px Activity Shown Inmentioning

confidence: 99%

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Preparation of an experimental mouse model lacking selenium-dependent glutathione peroxidase activities by feeding a selenium-deficient diet

Ueno

Shimokawa

Sekine-Suzuki

et al. 2021

J. Clin. Biochem. Nutr.

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Relatively young (4 week old) selenium deficient (SeD) mice, which lack the activity of selenium dependent glutathione peroxidase (GSH Px) isomers, were prepared using torula yeast based SeD diet. Mice were fed the torula yeast based SeD diet and ultra pure water. Several different timings for starting the SeD diet were assessed. The weekly time course of liver comprehensive GSH Px activity after weaning was monitored. Protein expression levels of GPx1 and 4 in the liver were measured by Western blot analysis. Gene expression levels of GPx1, 2, 3, 4, and 7 in the liver were measured by quantitative real time PCR. Apoptotic activity of thymocytes after hydrogen peroxide (H 2 O 2) exposure was com pared. Thirty day survival rates after whole body X ray irradiation were estimated. Pre birth or right after birth starting of the SeD diet in dams was unable to lead to creation of SeD mice due to neonatal death. This suggests that Se is necessary for normal birth and healthy growing of mouse pups. Starting the mother on the SeD diet from 2 weeks after giving birth (SeD trial 2w group) resulted in a usable SeD mouse model. The liver GSH Px activity of the SeD trial 2w group was almost none from 4 week olds, but the mice survived for more than 63 weeks. Protein and gene expression of GPx1 was suppressed in the SeD trial 2w group, but that of GPx4 was not. The thymocytes of the SeD trial 2w group were sensitive to H 2 O 2 induced apoptosis. The SeD trial 2w group was sensitive to whole body X ray irradiation compared with control mice. The SeD trial 2w model may be a useful animal model for H 2 O 2 /hydroperoxide induced oxidative stress.

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Section: Resultsmentioning

confidence: 99%

Section: Gsh-px Activity Shown Inmentioning

confidence: 99%

Preparation of an experimental mouse model lacking selenium-dependent glutathione peroxidase activities by feeding a selenium-deficient diet

Ueno

Shimokawa

Sekine-Suzuki

et al. 2021

J. Clin. Biochem. Nutr.

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“…The two different densities of localized OH generation did not vary according to the LET of carbonbeams; however, the yields of sparse OH, i.e. yield of DMPO-OH observed at the inflection point of 1 st and 2 nd phases, decreased with increasing LET [41,43]. On the other hand, the yield of DMPO-OH observed at the maximum DMPO density was not affected by LET.…”

Section: Detection/estimation Of Ohmentioning

confidence: 82%

“…The yield of H 2 O 2 expected from the primary G-value of H 2 O 2 of 0.7 [48] was 0.073 lmol/L/Gy. However, a larger yield of H 2 O 2 was experimentally observed as 0.26 ± 0.01 lmol/L/Gy in an X-ray-irradiated water sample [43]. For 20 keV/lm carbon-ion beams, the yield of H 2 O 2 was 0.20 ± 0.01 lmol/L/Gy.…”

Section: Detection Of H 2 Omentioning

confidence: 87%

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Heavy-ion beam-induced reactive oxygen species and redox reactions

Matsumoto

Ueno

Shoji

et al. 2021

Free Radical Research

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Quantification and local density estimation of radiation-induced reactive oxygen species (ROS) were described focusing on our recent and related studies. Charged particle radiation, i.e. heavyion beams, are currently utilized for medical treatment. Differences in ROS generation properties between photon and charged particle radiation may lead to differences in the quality of radiation. Radiation-induced generation of ROS in water was quantified using several different approaches to electron paramagnetic resonance (EPR) techniques. Two different densities of localized hydroxyl radical (OH) generation, i.e. milli-molar and molar levels, were described. Yields of sparse OH decreased with increasing linear energy transfer (LET), the yield total OH was not affected by LET. In the high-density, molar level, OH environment, OH can react and directly make hydrogen peroxide (H 2 O 2), and then possible to form a high-density H 2 O 2 cluster. The amount of total oxidation reactions caused by oxidative ROS, such as OH and hydroperoxyl radial (HO 2), was decreased with increasing LET. Possibilities of the sequential reactions were discussed based on the initial localized density at the generated site. Water-induced ROS have been well investigated. However, little is known about radiation-induced free radical generation in lipidic conditions. Radio-chemistry to understand the sequential radio-biological effects is still under development.

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Simultaneous removal of Cr and organic matters via coupling Cr-Fenton-like reaction with Cr flocculation: The key role of Cr flocs on coupling effect

Gao

Sun

et al. 2022

Chemosphere

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A quantitative analysis of carbon-ion beam-induced reactive oxygen species and redox reactions

Abstract: He received "SFRR Japan Award of Scientific Excellent" in 2017 in recognition of his outstanding work. ??

Cited by 20 publications

References 22 publications

Preparation of an experimental mouse model lacking selenium-dependent glutathione peroxidase activities by feeding a selenium-deficient diet

Preparation of an experimental mouse model lacking selenium-dependent glutathione peroxidase activities by feeding a selenium-deficient diet

Heavy-ion beam-induced reactive oxygen species and redox reactions

Simultaneous removal of Cr and organic matters via coupling Cr-Fenton-like reaction with Cr flocculation: The key role of Cr flocs on coupling effect

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