A quantitative assay of cortisol in human plasma by high performance liquid chromatography using a selective chemically bonded stationary phase

Berg, van den J Jan; Mol, CR Chris; Deelder, RS Rob; Thijssen, J. H. H.

doi:10.1016/0009-8981(77)90303-5

Cited by 65 publications

(4 citation statements)

References 13 publications

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“…Plasma cortisol samples were drawn in the morning and always at the same time for each patient, frozen, stored, and delivered to Astra Draco AB, Lund, for analysis using a high-performance liquid chromatography method (9).…”

Section: Laboratory Assessmentsmentioning

confidence: 99%

A Steroid Enema, Budesonide, Lacking Systemic Effects for the Treatment of Distal Ulcerative Colitis or Proctitis

Danielsson¹,

Löfberg²,

Persson³

et al. 1992

Scandinavian Journal of Gastroenterology

113

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The aim of this study was to evaluate whether budesonide enema (2 mg/100 ml) had a significantly better effect than placebo in the treatment of distal ulcerative colitis or proctitis. The trial was of controlled, randomized, double-blind design and included 41 treated patients. The treatment time was 4 weeks, with revisits after 2 and 4 weeks. If no improvement was seen, the patient could be switched over to open-label therapy with budesonide enema. Sigmoidoscopy, histology, blood chemistry, and diary cards were used for estimating the effect of treatment. The results showed that budesonide was superior to placebo. Sigmoidoscopy and biopsy scores improved significantly (p less than 0.01) in budesonide-treated patients compared with placebo. Significantly more patients switched over to open budesonide treatment in the placebo group owing to lack of efficacy compared with budesonide (p less than 0.001). No drug-related adverse experiences occurred, and there was no decrease in endogenous morning plasma cortisol levels. It is concluded that budesonide enema appears to be an effective and safe treatment for distal ulcerative colitis and proctitis.

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Section: Laboratory Assessmentsmentioning

confidence: 99%

A Steroid Enema, Budesonide, Lacking Systemic Effects for the Treatment of Distal Ulcerative Colitis or Proctitis

Danielsson¹,

Löfberg²,

Persson³

et al. 1992

Scandinavian Journal of Gastroenterology

113

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“…Serum glucagon, and serum and adrenal aldosterone were analysed with the RIA kits of Serono Diagnostics (Hypolab, SA, Switzerland). The glucagon antiserum 30K has I O O~~ cross-reactivity against glucagon and proglucagon, but it does not cross-react significantly against enteroglucagons (Weir et al 1973). Aldosterone was determined from serum after extraction with a 10-fold volume of diethyl ether and from the adrenals after extraction with 6 ml of dichloromethane.…”

Section: Methodsmentioning

confidence: 99%

“…Extraction of glucocorticoids. The glucocorticoids were extracted from I ml serum samples with a six-fold volume of dichloromethane (Van den Berg et al 1977) and from the adrenal glands by homogenizing (in a glass homogenizer) one gland from each animal in 6 ml of dichloromethane that contained 500 ng cortisol as an internal standard. After filtering through Whatman No.…”

Section: Methodsmentioning

confidence: 99%

Adrenal function of the rat in relation to peroral administration of xylitol: depression of aldosterone

Hämäläinen

Mäkinen

1987

Acta Physiologica Scandinavica

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The effect of peroral administration of xylitol (5% or 20% in food) on adrenal function was investigated in thirty-five Long-Evans male rats. The control rats were fed either a non-substituted stock diet or a 20% glucose diet. Glucose elevated and 20% xylitol reduced the growth of the rats (P less than 0.001), but 5% xylitol had no effect on the body weight. The concentrations of serum glucose and lactic acid decreased in rats fed 20% and 5% xylitol, respectively, but those of insulin, glucagon, corticosterone and aldosterone were not affected. In the adrenal glands, 20% xylitol loading was associated with increased epinephrine (P less than 0.05) and norepinephrine (P less than 0.001), but with decreased aldosterone (P less than 0.001) concentrations. The weights and histological picture of adrenal glands were normal. The urinary pH of xylitol-fed rats decreased significantly (P less than 0.01). Although peroral xylitol affected the levels of aldosterone and catecholamines, a normal glucocorticoid metabolism was permitted. The reduced aldosterone levels were regarded as secondary reactions, possibly resulting from alterations in electrolyte and/or acid-base balance. The increased catecholamine synthesis may be associated with the promoting effect of xylitol on intestinal calcium absorption.

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“…Cortisol in plasma was quantified at the Department of Bioatialytical Chemistry, Astra-Draco, by high-performance liquid chrotnatography (HPLC) (7). Paired ?-tests were used to compare cortisol levels after 1) corresponding doses of the two drugs and 2) each GCS treatment with placebo.…”

Section: Methodsmentioning

confidence: 99%

Systemic effects of two nasally administered glucocorticosteroids

Andersson

Johansson

1997

Allergy

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Two topical corticosteroids, budesonide (BUD) and beclomethasone dipropionate (BDP), both administered as suspensions in water, were investigated in healthy volunteers regarding influence on cortisol in plasma and urine (U-cortisol) after nasal application. In the first study, single doses of 200, 400, and 800 micrograms of BDP and BUD were given at 10:00 pm. In the second study, 100, 200, and 400 micrograms were given mornings and evenings for 4 days. In the single-dose study, none of the drugs or doses showed any significant influence on cortisol in plasma. However, U-cortisol decreased significantly after BUD 400 and 800 micrograms. In the multidose study, U-cortisol values were significantly reduced after all doses of BUD and the highest dose of BDP. The compounds tested showed different ability to cause measurable systemic effects after nasal application. The clinical implication is that the prescriber, when choosing a compound, should take the application site into consideration and should also be encouraged to find the lowest effective dose.

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A quantitative assay of cortisol in human plasma by high performance liquid chromatography using a selective chemically bonded stationary phase

Cited by 65 publications

References 13 publications

A Steroid Enema, Budesonide, Lacking Systemic Effects for the Treatment of Distal Ulcerative Colitis or Proctitis

A Steroid Enema, Budesonide, Lacking Systemic Effects for the Treatment of Distal Ulcerative Colitis or Proctitis

Adrenal function of the rat in relation to peroral administration of xylitol: depression of aldosterone

Systemic effects of two nasally administered glucocorticosteroids

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