A Quantitative Chromatographic Study of Nitrogenous Components of Shoot Apices

Steward, F. C.; Wetmore, Ralph H.; Thompson, John F.; Nitsch, J. P.

doi:10.2307/2439318

Cited by 35 publications

(9 citation statements)

References 8 publications

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“…The free acid was responsible for the very strong spot labelled C in the earlier paper (No. 79 on the maps of Steward et al, 1955). Suffice it to say here that the evidence of identity obtained by two of us (J.K.P.…”

Section: Results-y-hydroxy Y-methylglutamic Acid Inmentioning

confidence: 85%

“…This unidentified Substance C in Adiantum of Steward et al, (1954) is present in relatively large amount and may be isolated as a crystalline product. From its chromatographic behavior, its possible identity as a dicarboxylic amino acid, probably a hydroxydicarboxylic amino acid, was suspected.…”

Section: Results-y-hydroxy Y-methylglutamic Acid Inmentioning

confidence: 99%

“…Adiantum.-When first seen, the unidentified substance in the apex of Adiantum (Substance C of fig. 4, p. 129, Steward et al, 1954) was thought to be acid unstable, probably yielding glutamic acid on hydrolysis, because a shift in its position on the chromatograms seemed to occur after acid hydrolysis and no other ninhydrin-reactive substance appeared. This substance has now been submitted to an intensive investigation, the more chemical details of which will be published elsewhere.…”

Section: Results-y-hydroxy Y-methylglutamic Acid Inmentioning

confidence: 99%

“…methylglutamic acid to the methylglutamic acid which was chromatographically identical with v: methylglutamic acid (No. 148 on maps of Steward et al, 1955).…”

Section: Results-y-hydroxy Y-methylglutamic Acid Inmentioning

confidence: 99%

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The Nitrogenous Components of the Shoot Apex of Adiantum pedatum

Steward

Wetmore

Pollard

1955

American Journal of Botany

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THE P~ESENT communication is a sequel to a previous paper which described an investigation, by quantitative paper chromatography, of the nitrogen compounds which, when free, comprise the soluble nitrogen fraction and, when combined, the protein nitrogen of certain shoot apices. The earlier investigation revealed general effects of two kinds. First, the angiosperm shoot apices which were investigated differed greatly from the fern apex studied with respect to the range of soluble amino-nitrogen compounds that they contained. Second, there were evident signs that differences in composition in these apices occurred pari passu with development. The data here reported extend our knowledge with respect to Adiantum pedatum.The shoot apex of Adiantum as earlier reported was somewhat remarkable in contrast to that of Lupinus in that the alcohol soluble nitrogen consisted of relatively few identifiable amino acids and ami des present in quantity (aspartic, glutamic, yaminobutyric acids, valine, leucine and glutamine) and a substance, not identified, which was present in far greater quantity than any of these. The reexamination of the apex of Adiantum had the following objectives: (i ) To investigate the apex at a time when it was most actively growing. (ii l To determine the composition of various regions of the apex which were sampled according to their state of maturity. (iii) To shed further light on the chemical nature of the hitherto unidentified nitrogen compound that occurs in the Adiantum apex.MATERIALS AND METHODs.-Plants of Adiantum pedatum were purchased from the Fern Nursery of Isaac Langley Williams of Exeter, New Hampshire, after the leaves had died down in the autumn. The rhizomes were planted in flats and kept in a 4°C. cold room until brought to the greenhouses at Harvard University. They were grown in daylight supplemented by artifical light, the amount varying with the season. They were also given extra illumi-1

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Section: Results-y-hydroxy Y-methylglutamic Acid Inmentioning

confidence: 85%

Section: Results-y-hydroxy Y-methylglutamic Acid Inmentioning

confidence: 99%

Section: Results-y-hydroxy Y-methylglutamic Acid Inmentioning

confidence: 99%

“…methylglutamic acid to the methylglutamic acid which was chromatographically identical with v: methylglutamic acid (No. 148 on maps of Steward et al, 1955).…”

Section: Results-y-hydroxy Y-methylglutamic Acid Inmentioning

confidence: 99%

See 2 more Smart Citations

The Nitrogenous Components of the Shoot Apex of Adiantum pedatum

Steward

Wetmore

Pollard

1955

American Journal of Botany

Self Cite

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show abstract

“…The amino acid composition of a leaf may also undergo considerable change as the leaf ages. This fact was well illustrated by the results of Steward, Whetmore, Thompson & Nitsch (1954), who studied the composition of the leaves of white lupin, Lupinus albus, at different stages of their development from primordia to the fully expanded state. Asparagine represented a progressively increasing amount of the total soluble nitrogen of the leaf as its 26-2 4'2 L. FOWDEN development proceeded, whilst the basic amino acid, and glutamine, glutamic acid and y-aminobutyric acid all decreased in relative amount.…”

Section: (A) Amino Acid Concentrations In Relation To Plant Developmentmentioning

confidence: 74%