A quantitative determination of tocotrienols and tocopherols in palm oil by TLC‐GLC

Meijboom, P. W.; Jongenotter, G. A.

doi:10.1007/bf02671757

Cited by 21 publications

(4 citation statements)

References 8 publications

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“…The total tocopherol content, determined (as total reducing substances) by the vitamin E panel method (16), also showed high values for fat from these pulses. The unsaponifiable matter of the fat from these pulses showed an unusually large amount of tocopherol as examined by qualitative thinlayer chromatography (TLC) (17). The spots on a TLC plate that correspond to standard tocopherols produced a pink color when sprayed with a mixture of 0.2% ferric chloride and 0.5% dipyridyl in ethanol.…”

Section: Resultsmentioning

confidence: 99%

Tocopherol and fatty acid composition of some Indian pulses

Krishna

Prabhakar

Aitzetmüller³

1997

J Americ Oil Chem Soc

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The tocopherol and tocotrienol composition of the Indian pulses Bengal gram (Cicer arietinum L.), blackgram (Vigna mungo L.), green gram (Vigna radiata L.), and horse gram (Dolichos biflorus L.) were studied. The total tocopherol content ranged from 230 to 1567 mg/100 g fat, while the tocopherol content of the pulses as a whole ranged from 6.76 to 12.54 mg/100 g seed. Presence of such a high amount of tocopherol, both in the oil fraction of Indian pulses and in an oil fraction of a food material, is being reported for the first time. The fatty acid composition of the fat extracted from these pulses showed substantial amounts of unsaturated fatty acids (Bengal gram, 88.7%; black gram, 82.9%; green gram, 64.3%; and horse gram, 66.9%). These pulses contained 3.8 to 49.1% linolenic acid in the fat.

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Section: Resultsmentioning

confidence: 99%

Tocopherol and fatty acid composition of some Indian pulses

Krishna

Prabhakar

Aitzetmüller³

1997

J Americ Oil Chem Soc

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“…The separated components were visualized with 2, 7-dichloro-fluorescein in ethanol 20) . The Rf values of long chain alcohols and sterols were 0.4 and 0.3 respectively.…”

Section: Separation Of Long Chain Fatty Alcohols From Sterolsmentioning

confidence: 99%

Investigation of Liquid Wax Components of Egyptian Jojoba Seeds

El-Mallah

El-Shami

2009

J. Oleo Sci.

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Egyptian jojoba seeds newly cultivated in Ismailia desert in Egypt promoted us to determine its lipid components. Fatty alcohols, fatty acids, wax esters and sterols patterns were determined by capillary GLC whereas, tocopherols profile, isopropenoid alcohols and sterylglycosides were determined by HPLC. The Egyptian seeds are rich in wax esters (55 %) with fatty alcohols C20:1and C22:1 as major components and amounted to 43.0 % and 45.6 % respectively followed by C24:1 and C18:1(9.6 % and 1.3 % respectively). The fatty acids profile showed that C20:1 is the major constituent (60 %) followed by C18:1and C22:1 (14.5 and 11.8 % respectively) whereas C24:1 was present at low concentration amounted to 1.6 %. In addition, the Egyptian jojoba wax contained C18:2 fatty acid at a level of 8.7 %. Wax esters composition showed that the local wax had C42 and C40 esters as major components amounted to 51.1 and 30.1 % respectively. Also, it had C44 and C38 at reasonable amounts (10.0 and 6.3 % respectively). Whereas C36 and C46 were present at lower concentrations amounted to 1.4 and 1.1 respectively. The sterols analysis showed the presence of campe-, stigma-, b-sito-, and isofuco-sterol amounting to 18.4 %, 6.9 %, 68.7 %, and 6.0 % respectively. The tocopherols pattern revealed that the local seed wax contained g-tocopherol as major constituent (79.2 %) followed by a-tocopherol (20.3 %). b-tocopherol as well as dtocopherol were found as minor constituents. The isopropenoid alcohols and the sterylglycosides (free and acylated) were not detected. The wax is proposed to be used in oleo chemistry and cosmetics.

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“…Quantitative determination of a-T and its oxidation products (TOP) can be used to measure the degree of cell membrane peroxidation and the metabolic pathways of a-T, and useful in the prediction of the stability of oils (7,8). Gas chromatography, column chromatography and thin layer chromatography were methods used commonly for the separation of a-T and TOP (9)(10)(11)(12). These methods have serious limitations because of their comparatively low sensitivity, long analysis time and possibility of increased oxidation.…”

Section: Lipids 23 359-361 (1988)mentioning

confidence: 99%

Separation of α‐tocopherol and its oxidation products by high performance liquid chromatography

Csallany

1988

Lipids

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A very sensitive high performance liquid chromatographic (HPLC) method was developed for the separation of alpha-tocopherol (alpha-T) and its five oxidation products: alpha-tocopheryl quinone (TQ), dimer (D), dihydroxy dimer (DHD), trimer (T) and 9-methoxy-alpha-tocopherone commonly called alpha-tocopheroxide (TO). The separation was achieved on a normal-phase silica-based column (Ultrasphere-Si), using a mobile phase of hexane/chloroform/isopropanol (95:4.5:0.5, v/v/v) at a flow rate of 0.4 ml/min, and the eluants were monitored simultaneously at their maximum absorptions using a variable-wavelength UV detector. The minimum detection limit is 0.01 microgram for alpha-T, TQ and TO, 0.05 microgram for DHD and D, and 0.1 microgram for T/injection. This normal-phase method has the combined advantages of being very sensitive, fast and capable of separating all six compounds at the same time.

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A quantitative determination of tocotrienols and tocopherols in palm oil by TLC‐GLC

Cited by 21 publications

References 8 publications

Tocopherol and fatty acid composition of some Indian pulses

Tocopherol and fatty acid composition of some Indian pulses

Investigation of Liquid Wax Components of Egyptian Jojoba Seeds

Separation of α‐tocopherol and its oxidation products by high performance liquid chromatography

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