A quantitative evaluation of two methods for preserving hair samples

Roon, David A.; Waits, Lisette P.; Kendall, Katherine C.

doi:10.1046/j.1471-8286.2003.00358.x

Cited by 55 publications

(50 citation statements)

References 11 publications

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“…These characteristics most likely make them a preferred place for marking and rubbing purposes. Considerable effort has been invested in identifying the most suitable storage methods for the preservation of genetic material from bears that will guarantee the best DNA amplification rates (Murphy et al 2000(Murphy et al , 2003Roon et al 2003). In contrast, little is known about the effect of field conditions on DNA amplification rates.…”

Section: Discussionmentioning

confidence: 99%

“…Hair samples were placed in paper envelopes without contacting human skin and then stored at room temperature in ziplock bags with silica gel (Roon et al 2003). For every collected hair sample, DNA extractions were performed using the DNeasy Blood and Tissue kits (QIAGEN, Hilden, Germany) following the manufacturer's instructions.…”

Section: Individual and Sex Identification Of Brown Bearsmentioning

confidence: 99%

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Noninvasive genetic studies of brown bears using power poles

Karamanlidis

Drosopoulou

Hernando³

et al. 2010

Eur J Wildl Res

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One difficulty in the conservation of endangered wildlife is the lack of reliable information on its status. This lack of knowledge can often be attributed to financial and logistic constraints as well as the lack of trained personnel to collect data. We test a simple method to study bears in the southern Balkans by inspecting power poles, which are used by bears for marking and rubbing purposes. We created a network of barbed-wire fitted poles for the collection of hair samples, evenly distributed throughout six study areas. During 87 sampling sessions in the main study area, we collected 191 samples and identified six microsatellite loci that were variable enough for individual bear identification. The most and best-quality hair samples were collected during the mating period, and DNA was most successfully extracted from samples remaining <4 weeks in the field. In the six study areas, we identified 47 bears. An advantage of using power poles for hair sampling is their availability and accessibility; no bait is required, and the network can be easily set up. A drawback may be an unequal capture probability of sex and age classes of bears. Despite this limitation, using power poles proved to be a simple and cheap method for the noninvasive genetic study of bears that did not require any prior knowledge on habitat use and activity patterns. The method is suitable for large-scale surveys to estimate distribution and relative densities of bears and could also be applied for studying other species.

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Section: Discussionmentioning

confidence: 99%

Section: Individual and Sex Identification Of Brown Bearsmentioning

confidence: 99%

Noninvasive genetic studies of brown bears using power poles

Karamanlidis

Drosopoulou

Hernando³

et al. 2010

Eur J Wildl Res

View full text Add to dashboard Cite

show abstract

“…Many of the collection techniques already developed for noninvasive, DNA-based approaches (e.g., hair traps [Pauli et al 2008] or hair snares [DePue and Ben-David 2007]) can be used with the isotopic method we describe. Isotopic labeling offers the added benefit that, unlike DNA (Roon et al 2003), the keratin is resilient to degradation (Macko et al 1999).…”

Section: Discussionmentioning

confidence: 99%

Hair Sampling Techniques for River Otters

DePue¹,

Ben‐David²

2007

J Wildl Manag

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River otter (Lontra canadensis) populations have been difficult to monitor and information on densities is lacking throughout their range. To obtain DNA‐based population estimates of river otters we developed 2 traps to capture hair; a modified body‐snare and a modified foot‐hold trap. Of 82 traps activated 77 captured hairs (94%). Traps snagged 3–20 guard hairs per capture. Our capture rates of otter hair ranged from one capture per 3.6 trap nights to one capture per 156.6 trap‐nights. Our traps provide an effective, noninvasive technique for obtaining hair DNA from individual river otters.

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“…Hair samples were stored at -20°C in individual envelopes, each of which was packaged again in plastic waterproof bags (Ziploc type) as described by Roon et al (2003).…”

Section: Sample Collection and Conservationmentioning

confidence: 99%

Reliability of non-invasive tissue sampling methods for DNA extraction in rabbits (Oryctolagus cuniculus)

et al. 2012

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Deoxyribonucleic acid (DNA) can be extracted from different tissue sources. The most common is blood, but in some situations it can be easier to take a biopsy. In some cases when it is difficult to capture animals, especially in wild populations, faeces and hairs can be considered as a source of DNA. This paper presents a pilot study conducted to compare the applicability of invasive and non-invasive sampling methods for extracting DNA for use in genetic studies of rabbits (Oryctolagus cuniculus). The study included 24 rabbits from the INRA 1001 strain. Blood, hair, ear biopsies and faeces were collected and used as DNA sources. Our aim was to verify the quantity of DNA obtained from different tissues using 2 or 3 types of extraction. DNA was obtained for all tissue types and all extraction methods. DNA extraction was shown to be optimal with the LGC (Laboratory of Cellular Genetics) blood extraction method. With regard to non-invasive methods, DNA extraction for hair using the LGC protocol and QIAamp ® DNA mini kit gave very low quantities of DNA that could not be used for PCR reactions. The Chelex extraction protocol gave good results for PCR but could not be quantified. DNA extracted from faeces is a viable source of DNA for determining individual genotypes. The use of such non-invasive samples as a source of genetic material is a recent and very promising technique, especially for the study of endangered species, but these techniques are still too unreliable and costly to altogether replace invasive techniques when the latter are possible.

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A quantitative evaluation of two methods for preserving hair samples

Cited by 55 publications

References 11 publications

Noninvasive genetic studies of brown bears using power poles

Noninvasive genetic studies of brown bears using power poles

Hair Sampling Techniques for River Otters

Reliability of non-invasive tissue sampling methods for DNA extraction in rabbits (Oryctolagus cuniculus)

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