A quantitative histochemical study of lactate dehydrogenase and succinate dehydrogenase activities in the membrana granulosa of the ovulatory follicle of the rat

Zoller, Lawrence C.; Enelow, Richard I.

doi:10.1007/bf01003969

Cited by 13 publications

(4 citation statements)

References 23 publications

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“…Both side-chain cleavage of cholesterol and aromatization of androgens to oestrogens require molecular oxygen (Ryan, 1959;Halkerston et al, 1961). A gradient of biosynthetic activity has been demonstrated by the preferential distribution of key enzymes in the peripheral granulosa layer of rat Graafian follicles (Zoller andWeisz, 1978, 1979;Zoller and Enelow, 1983), which is also optimally placed for the diffusion of hormonal products into neighbouring capillaries. It is, however, recognised that pre-antral follicles are much less competent to perform steroid biosynthesis than are Graafian types (Roy and Greenwald, 1985), and the functional significance of the oxygen gradient in these stages therefore requires further investigation.…”

Section: Discussionmentioning

confidence: 97%

Oxygen concentration gradient across the ovarian follicular epithelium: model, predictions and implications

1986

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A mathematical model has been devised for predicting the oxygen concentration gradient across the epithelium of ovarian follicles at pre-antral stages. Most dissolved oxygen entering the follicle by diffusion is evidently consumed in the outer layer of cells; little reaches the oocyte. Even when the rate of consumption or the diffusion coefficient of oxygen was adjusted by an order of magnitude to favour oxygen penetration, the concentration gradient into the follicle remained steep. On the basis of measurements of ovine granulosa cell respiration in vitro, the model predicts that a large pre-antral follicle with a radius of 0.15 mm consumes oxygen at the rate of 0.22 nmol min-1.

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Section: Discussionmentioning

confidence: 97%

Oxygen concentration gradient across the ovarian follicular epithelium: model, predictions and implications

1986

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“…Differences between granulosa and cumulus cells have been reported by other authors. Morphologically, the two cellular types differ (rat and hamster: Zoller and Enelow, 1983;Zoller, 19841, and they do not undergo atresia at the same rate (sheep: Hay et al, 1976). Significant differences in the proteins secreted by ovine granulosa and cumulus cells have been reported (sheep: Moor and Crosby, 1987).…”

Section: Discussionmentioning

confidence: 99%

Qualitative and quantitative changes in protein sythesis of bovine follicular cells during the preovulatory period

Rabahi

Monniaux

Pisselet

et al. 1991

Molecular Reproduction Devel

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To understand the mechanisms governing oocyte maturation better, the effects of the gonadotropin surge were studied on follicular cells of bovine preovulatory follicles. For this purpose, qualitative and quantitative changes in protein synthesis by both granulosa cells and cumulus cells were compared relative to the luteinizing hormone (LH) surge and the resumption of meiosis in the oocyte. Follicular cells were collected at different times before and up to 25 hr after the LH surge. For each individual preovulatory follicle, granulosa and cumulus cells were incubated separately for 3 hr with 3H-methionine or with 35S-methionine. Newly synthesized cytosolic proteins from granulosa and cumulus cells and proteins secreted into the medium were analyzed by polyacrylamide gel electrophoresis. The radioactivity was measured by liquid scintillation counting after slicing of the gels or revealed by fluorography. Three major peaks of the newly synthesized proteins, with molecular weights of 76, 56, and 30 kDa, were studied throughout the preovulatory period. After the LH surge, the overall level of protein synthesis increased in granulosa cells. In addition, the pattern of cytosolic proteins in granulosa cells changed, and, in particular, the relative synthesis of the 30 kDa peak decreased. These changes in cytosolic protein synthesis may be due to the action of LH since they could be reproduced in vitro in LH-stimulated granulosa cells. A predominant peak of 56 kDa was secreted by granulosa cells throughout the experimental period. No significant change was observed in proteins synthesized by cumulus cells under the same experimental conditions. The amounts of radioactivity incorporated into the three major proteins secreted by granulosa cells, however, were correlated significantly with the amounts of radioactivity incorporated by similar proteins synthesized by cumulus cells. These results indicate that cumulus cells respond differently from granulosa cells to the gonadotropin surge but not in an independent manner.

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“…In fact, reverse transcriptase subunit was observed only in the cells localized close to the antrum. The presence of a functional differentiation within somatic follicular cells was also suggested by several authors who demonstrated a different expression of many genes, distribution of LH receptors (Amsterdam et al 1975;Bortolussi et al 1977), and location of steroidogenic enzymes (Zoller and Weisz 1978;Zlotkin et al 1986;Tabarowski and Szoltys 1987) and housekeeping enzymes as well (Zoller and Weisz 1979;Zoller and Enelow 1983). It has been proposed, in this context, that cells localized in the basal zone of the mural layer are better differentiated than the antrally situated cells (Amsterdam and Rotmensch 1987).…”

Section: )mentioning

confidence: 94%

Expression of Telomerase Reverse Transcriptase Subunit (TERT) and Telomere Sizing in Pig Ovarian Follicles

Russo

Berardinelli

Martelli

et al. 2006

J Histochem Cytochem.

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(DF) S U M M A R Y Telomerase is crucial for chromosome stability because it maintains telomere length. Little is known about telomerase in ovarian follicles, where an intense cell division is crucial to sustain estrous cycle and to drive oocyte development. The present research was performed to detect, by immunohistochemistry, the distribution of telomerase catalytic subunit (TERT) during folliculogenesis and to study the effect of TERT expression on telomeres. To this aim, telomere length has been measured on fluorescence in situ hybridization (FISH)-processed sections either in follicular or in germ cells. In primary and preantral follicles, TERT was observed in granulosa and in germ cells, with a typical nuclear location. During antral differentiation, only somatic cells close to the antrum (antral layer) and cumulus cells maintained TERT expression. The relative oocytes located TERT in the ooplasm independent from the process of meiotic maturation. FISH results indicate that a correlation exists between TERT expression and telomere size. In fact, progressively bigger telomeres were observed from preantral to antral follicles where longer structures were recorded in cells of the cumulus oophorus and of the antral layer than those of the basal one. Stable and elongated telomeres were detected in fully grown oocytes that lost the functional TERT distribution within the nucleus. (J Histochem Cytochem 54:443-455, 2006)

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A quantitative histochemical study of lactate dehydrogenase and succinate dehydrogenase activities in the membrana granulosa of the ovulatory follicle of the rat

Cited by 13 publications

References 23 publications

Oxygen concentration gradient across the ovarian follicular epithelium: model, predictions and implications

Oxygen concentration gradient across the ovarian follicular epithelium: model, predictions and implications

Qualitative and quantitative changes in protein sythesis of bovine follicular cells during the preovulatory period

Expression of Telomerase Reverse Transcriptase Subunit (TERT) and Telomere Sizing in Pig Ovarian Follicles

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