A quantitative immunoperoxidase procedure employing energy dispersive x-ray analysis.

Siegesmund, Kenneth A.; Yorde, Donald E.; Dragen, R

doi:10.1177/27.9.383829

Cited by 10 publications

(6 citation statements)

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“…The use of gold as an immunostaining agent was first documented in 1971 when Faulk and Taylor used antibody coated AuNPs to visualise specific antigens using TEM [392]. Relying on the 'lock and key' mechanisms of antibody-antigen interactions in combination with the high electron density properties of gold, immunolabelling methods have expanded with multiple labelling possibilities (using different sized AuNPs) [393], and adaptations have been made for visualisation using SEM [394,395] and dark field microscopy [374].…”

Section: Gold Nanoparticles As Bioimaging Agentsmentioning

confidence: 99%

Size, shape and surface chemistry of nano-gold dictate its cellular interactions, uptake and toxicity

Carnovale

Bryant

Shukla

et al. 2016

Progress in Materials Science

147

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Colloidal gold is undoubtedly one of the most extensively studied nanomaterials, with 1000s of different protocols currently available to synthesise gold nanoparticles (AuNPs). While developments in the synthesis of AuNPs have progressed rapidly in recent years, our understanding of their biological impact, with particular respect to the effect of shape, size, surface characteristics and aggregation states, has struggled to keep pace. It is generally agreed that when AuNPs are exposed to biological systems, these parameters directly influence their pharmacokinetic and pharmacodynamic properties by influencing AuNPs distribution, circulation time, metabolism and excretion in biological systems.However, the rules governing these properties, and the science behind them, are poorly understood. Therefore, a systematic understanding of the implications of these variables at the nano-bio interface has recently become a topic of major interest. This Review Article attempts to ignite a discussion around the influence of different physico-chemical parameters on biological activity of AuNPs, while focussing on critical aspects of cellular interactions, uptake and cytotoxicity. The review also discusses emerging trends in AuNPs uptake and toxicity that are leading to technological advances through AuNPs-based therapy, diagnostics and imaging.

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Section: Gold Nanoparticles As Bioimaging Agentsmentioning

confidence: 99%

Size, shape and surface chemistry of nano-gold dictate its cellular interactions, uptake and toxicity

Carnovale

Bryant

Shukla

et al. 2016

Progress in Materials Science

147

View full text Add to dashboard Cite

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“…In immunohistochemical staining, DAB is known to be oxidized by hydrogen peroxide (H 2 O 2 ) in the presence of HRP that forms a brown deposition, representing the location of the HRP for light microscopy. Intensifications of DAB deposition sites have been reported with various heavy metallic ions as well as gold chloride 19 , 20 , 22 – 25 . The crucial binding ability between gold chloride and the immunochemical reaction product of DAB has been indicated by energy dispersive X (EDX)-ray analysis 25 .…”

Section: Resultsmentioning

confidence: 99%

In situ strategy for biomedical target localization via nanogold nucleation and secondary growth

et al. 2021

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Immunocytochemistry visualizes the exact spatial location of target molecules. The most common strategy for ultrastructural immunocytochemistry is the conjugation of nanogold particles to antibodies as probes. However, conventional nanogold labelling requires time-consuming nanogold probe preparation and ultrathin sectioning of cell/tissue samples. Here, we introduce an in situ strategy involving nanogold nucleation in immunoenzymatic products on universal paraffin/cryostat sections and provide unique insight into nanogold development under hot-humid air conditions. Nanogold particles were specifically localized on kidney podocytes to target synaptopodin. Transmission electron microscopy revealed secondary growth and self-assembly that could be experimentally controlled by bovine serum albumin stabilization and phosphate-buffered saline acceleration. Valuable retrospective nanogold labelling for gastric H+/K+-ATPase was achieved on vintage immunoenzymatic deposits after a long lapse of 15 years (i.e., 15-year-old deposits). The present in situ nanogold labelling is anticipated to fill the gap between light and electron microscopy to correlate cell/tissue structure and function.

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“…It is conceivable that all metal salts bind to polyDAB but that the co‐ordination chemistry of the product dictates whether it is catalytic or not. For example, both gold (Siegesmund et al , 1979; Newman et al , 1983a) and platinum (de Bruijn et al , 1986; Hiraoka & Hirai, 1992, 1995) salts have been demonstrated to bind to polyDAB and both promote the catalysis of silver reduction. By contrast, phosphotungstic acid has been reported to increase the electron opacity of polyDAB (Newman et al , 1983a) but was not observed to catalyze silver reduction in the experiments reported here.…”

Section: Discussionmentioning

confidence: 99%

The amplification of polymerized diaminobenzidine with physical developers: sensitizing effects of transition metal salts and sulphide

Ruhland

Jasani

2010

Journal of Microscopy

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SummaryAmplification of metal-complexed polymerized diaminobenzidine by two light-insensitive physical developers was systematically examined in a dot blot model system following either polymerizing diaminobenzidine in the presence of transition metal salts or applying the metal salts post-diaminobenzidine polymerization. The effect of sodium sulphide treatment on subsequent amplification was also investigated. Those metal-diaminobenzidine complexes that facilitated the most powerful amplification were subsequently tested in an immunohistochemical setting. The most dramatic amplification of polymerized diaminobenzidine was observed following its post-polymerization treatment with salts of platinum alone, or gold or vanadium with subsequent sulphide treatment, and allowed previously invisible quantities of polymerized diaminobenzidine to be clearly seen. Three other transition metal salts also improved the amplification of polymerized diaminobenzidine but to a lesser degree, namely nickel alone, and silver or rhodium with subsequent sulphide treatment. Sensitivity was comparable with the colloidal gold-silver amplification system.

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A quantitative immunoperoxidase procedure employing energy dispersive x-ray analysis.

Cited by 10 publications

References 0 publications

Size, shape and surface chemistry of nano-gold dictate its cellular interactions, uptake and toxicity

Size, shape and surface chemistry of nano-gold dictate its cellular interactions, uptake and toxicity

In situ strategy for biomedical target localization via nanogold nucleation and secondary growth

The amplification of polymerized diaminobenzidine with physical developers: sensitizing effects of transition metal salts and sulphide

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