A Quantitative Method to Measure Telomerase Activity by Bioluminescence Connected with Telomeric Repeat Amplification Protocol

Xu, Shunqing; He, Min; Yu, Hongping; Cai, Xiaokun; Tan, Xi; Lü, Bin; Shu, Baihua

doi:10.1006/abio.2001.5418

Cited by 169 publications

(96 citation statements)

References 27 publications

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“…[4][5][6][7][8] On the other hand, the utilization of the deprotonated form of the anion receptor for cation sensing has been seldom investigated. [9] Recently, a few carbazole-based acyclic receptors were reported for both fluorogenic and chromogenic sensing of anions, [10] although carbazole moieties have not yet been used in macrocyclic receptors.…”

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confidence: 99%

cyclo‐Bis(urea‐3,6‐dichlorocarbazole) as a Chromogenic and Fluorogenic Receptor for Anions and a Selective Sensor of Zinc and Copper Cations

Ahmed

Geronimo

Hwang

et al. 2011

Chemistry A European J

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Fluorogenic and chromogenic receptors for both cations and anions [1, 2] have attracted a considerable amount of attention due to the simplicity in recording the optical response upon formation of the host-guest complex. Considerable advancements have been achieved towards the development of highly selective receptors by fine tuning the optical response of the receptor to a particular cation or anion. In this context, many acyclic and macrocyclic neutral receptors having hydrogen binding units such as pyrrole, urea, thiourea, amide, amine, phenol, and Lewis acid are widely utilized.[3] It is quite evident that deprotonation of the acidic À NH group of pyrrole, imidazole, urea, or thiourea moieties upon complexation with fluoride, hydroxide, and pyrophosphate anions is crucial for the visual and chromogenic sensing of these anions. [4][5][6][7][8] On the other hand, the utilization of the deprotonated form of the anion receptor for cation sensing has been seldom investigated. [9] Recently, a few carbazole-based acyclic receptors were reported for both fluorogenic and chromogenic sensing of anions, [10] although carbazole moieties have not yet been used in macrocyclic receptors. The dual optical sensing capability of carbazole-urea receptors and the ease of deprotonation of the acidic À NH groups indicate that deprotonated carbazole-urea receptors could be effectively used for cation sensing. Toward this direction, herein we report a novel macrocyclic carbazoleurea compound that can detect fluoride and pyrophosphate through colorimetric/chromogenic and fluorogenic changes and shows selective fluorescent discrimination of H 2 PO 4 À . Furthermore, the deprotonated carbazole-urea receptor is used for highly selective ratiometric sensing of Zn 2 + by means of fluorescent changes and of Zn 2 + and Cu 2 + by means of colorimetric changes.1,8-Diamino-3,6-dichlorocarbazole (a) was synthesized according to literature procedures (Scheme 1).[11] The reaction of a with N,N-carbonyldiimidazole in dimethylsulfoxide (DMSO) solution results in conversion into the white powder cyclo-bis(urea-3,6-dichlorocarbazole) (1; Scheme 1) with 92 % yield.cyclo-Bis(urea-3,6-dichlorocarbazole) (1) is a new fluorogenic and chromogenic chemosensor for anions. The anion sensing capacity of 1 is studied by visual inspection of color changes in a DMSO-0.5 % water solution (10 mm) upon addition of 100 equivalents of tetrabutylammonium (TBA) anion salts (Figure 1 a). The corresponding absorption spec- , AcO À ) as a 1:100 equivalent ratio in a DMSO-0.5 % water solution. Corresponding b) absorption and c) fluorescence spectra (slit width = 3 nm; excitation = 365 nm).

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confidence: 99%

cyclo‐Bis(urea‐3,6‐dichlorocarbazole) as a Chromogenic and Fluorogenic Receptor for Anions and a Selective Sensor of Zinc and Copper Cations

Ahmed

Geronimo

Hwang

et al. 2011

Chemistry A European J

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“…研究表明, PPi 能够参与许多生命过程中的能量传递以及新陈代谢 [1] , 如在 DNA 聚合酶的催化下参与 DNA 的复制, 据此可通过对 PPi 的传感实现 DNA 序列的实时测定 [2] . PPi 还可作为潜在的生物标记物应用于癌症 [3] 、软骨钙质沉着病 [4] 以及关节炎 [5,6] [16,17] , 一般只有在极性有机溶剂中才有较好的选择性和灵敏度.…”

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Research Progress in the Sensing Ensembles for Pyrophosphate

Wang¹,

Chu²,

Chen³

et al. 2016

Chin. J. Org. Chem.

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“…The detection of released pyrophosphate has been investigated as a real-time DNA sequencing method [37]. Studies on telomerase activity (a biomarker for cancer) by evaluating the amount of pyrophosphate are also important in cancer research [38]. Furthermore, the pyrophosphate level in synovial fluids has been correlated with the occurrence of calcium pyrophosphate dihydrate disease, a rheumatologic disorder [39].…”

Section: Introductionmentioning

confidence: 99%