A Quantitative Method to Monitor Reactive Oxygen Species Production by Electron Paramagnetic Resonance in Physiological and Pathological Conditions

Mrakic‐Sposta, Simona; Gussoni, M.; Montorsi, Michela; Porcelli, Simone; Vezzoli, Alessandra

doi:10.1155/2014/306179

Cited by 66 publications

(76 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Oxidative stress biomarkers at baseline (262 m) were in agreement with previous values estimated for a healthy population17. Hypobaric hypoxia exposure lead to an increase of indices of oxidative stress; namely, increased concentrations of ROS production, TBARS, 8-isoPGF2α (lipid oxidation products and potential disease mediators), plus a decrease in TAC (the sum of aqueous and lipid soluble low-molecular weight antioxidants).…”

Section: Resultssupporting

confidence: 88%

“…There is some evidence from cell and tissue experiments that acute hypoxia induces accumulation of ROS in cells and tissues over time1020. This seems to be supported by our results, showing a progressive increase in ROS within 24 h. The prolonged imbalance between ROS production and antioxidant scavenging induction indicates the appearance of oxidative stress-related damages in DNA, proteins and lipids1721. Biological membranes constitute, in fact, a preferential target for ROS, largely because of their unsaturated fatty acid content.…”

Section: Discussionsupporting

confidence: 84%

See 1 more Smart Citation

Oxidative stress response to acute hypobaric hypoxia and its association with indirect measurement of increased intracranial pressure: a field study

Strapazzon

Malacrida

Vezzoli

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

High altitude is the most intriguing natural laboratory to study human physiological response to hypoxic conditions. In this study, we investigated changes in reactive oxygen species (ROS) and oxidative stress biomarkers during exposure to hypobaric hypoxia in 16 lowlanders. Moreover, we looked at the potential relationship between ROS related cellular damage and optic nerve sheath diameter (ONSD) as an indirect measurement of intracranial pressure. Baseline measurement of clinical signs and symptoms, biological samples and ultrasonography were assessed at 262 m and after passive ascent to 3830 m (9, 24 and 72 h). After 24 h the imbalance between ROS production (+141%) and scavenging (−41%) reflected an increase in oxidative stress related damage of 50–85%. ONSD concurrently increased, but regression analysis did not infer a causal relationship between oxidative stress biomarkers and changes in ONSD. These results provide new insight regarding ROS homeostasis and potential pathophysiological mechanisms of acute exposure to hypobaric hypoxia, plus other disease states associated with oxidative-stress damage as a result of tissue hypoxia.

show abstract

Section: Resultssupporting

confidence: 88%

Section: Discussionsupporting

confidence: 84%

Oxidative stress response to acute hypobaric hypoxia and its association with indirect measurement of increased intracranial pressure: a field study

Strapazzon

Malacrida

Vezzoli

et al. 2016

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…For the quantification of reactive oxygen species in the muscle tissue, the protocol recommended by Mrakic-Sposta et al [20] was followed. The pieces of the extensor carpi radialis muscle immersed in liquid nitrogen were transported to the Laboratory of Biochemistry and Protein Chemistry at the University of Brasília to prepare the samples for the paramagnetic resonance spectroscopy analyses at the Laboratory of Electron Paramagnetic Resonance at the Institute of Physics.…”

Section: Methodsmentioning

confidence: 99%

Reactive oxygen species and evaluation of the quality of carcasses and beef meat from cold storage slaughterhouses located in the Federal District and Surroundings (Brazil)

Sousa

et al. 2018

Preprint

View full text Add to dashboard Cite

26The aim of this study was to detect reactive oxygen species and evaluate the quality 27 of carcasses and beef meat from cold storage slaughterhouses located in the Federal District 28 and Surroundings. Information was obtained on the gender, breed and age of each animal, 29 as well as the distance travelled (km) and time (h) from the property to the cold storage 30 slaughterhouse. Data and samples were obtained from a total of 33 animals and their 31 respective carcasses. Fragments of the extensor carpi radialis muscle were extracted from all 32 the carcasses to analyze the presence of reactive oxygen species (ROS), and samples of the 33 Longissimus dorsi muscle to perform the 24h post-mortem pH, colorimetric test, cooking 34 loss and drip loss assessment and to measure the shear force. The presence of hematomas 35 was detected in 28 carcasses, where the tail and croup (17/33) and flank (17/33) regions were 36 the most affected. The electron paramagnetic resonance indicated an average of 52.59 ROS/g 37 in the analyzed pieces. The meat quality tests indicated averages of: 5.8 for the 24h post-38 mortem pH, L*29.34, a* 2.52 and b* 1.31 in calorimetry, 2.30 kg/f for the shearing force, 39 11.75% of cooking losses and 1.88% of drip losses. The statistical analyses demonstrated a 40 tendency to positive correlation between the presence of hematomas with and the amount 41 of ROS, and between the presence of hematomas and pH value. Furthermore, statistically, 42 female gender was one of the influential factors on the tenderness value. According to the 43 results, it was concluded that the meat evaluated in this study meets the desirable quality 44 parameters and it was possible to detect reactive oxygen species in the samples of muscle 45 tissue. 3 46 Introduction 47 Brazil's livestock farming stands out as an important sector of the national economy 48 and in the year 2016 it represented approximately 24% of the Gross Domestic Product [1]. 49 In 2017 Brazil kept its spotlight in the international market as the world's greatest beef meat 50 exporter [1]. 51 To maintain its market competitiveness, the country has invested in the productivity 52 and quality of the final product offered to the consumer [2]. In beef , tenderness, flavor, 53 juiciness and color are desired as determining characteristics for the purchase decision, 54 which are susceptible to changes due to intrinsic factors such as the gender, age and breed, 55 and extrinsic factors such as nutrition and stress during handling [3]. 56 Studies on meat quality often correlate these parameters when evaluating the 57 characteristics of the meat produced by crossbreeding and the comparison of age and diet 58 types [4,5,6,7], where the main analyzed characteristics are the pH, colorimetry and shearing 59 force [8,9,10] as well as water retention capacity when measured by evaluating cooking and 60 drip losses [4]. 61 Still related to the meat quality is the pre-slaughter handling performed by the farms 62 and industries of meat, which can generate stress, contusio...

show abstract

“…(i) ROS Detection. ROS production rate was assessed using the methods of Mrakic-Sposta et al [7,25,35,67] with an X-band EPR instrument (E-Scan-Bruker BioSpin, GmbH, MA USA), using 50 μL of capillary blood withdrawn from the fingertip and treated immediately with probe solution CMH (1-hydroxy-3-methoxycarbonyl-2,2,5,5-tetra-methylpyrrolidine). All samples were stabilized at 37°C by the Temperature and Gas Controller "Bio III" unit, interfaced to the spectrometer.…”

Section: Blood Measurementsmentioning

confidence: 99%

“…All samples were stabilized at 37°C by the Temperature and Gas Controller "Bio III" unit, interfaced to the spectrometer. All spectra were collected by acquisition parameters previously reported [7,25,35,67], and Bruker software (Win EPR System, V. 2.11) was adopted for the analysis.…”

Section: Blood Measurementsmentioning

confidence: 99%

Acute Effects of Triathlon Race on Oxidative Stress Biomarkers

Mrakic‐Sposta

Gussoni

Vezzoli

et al. 2020

Oxidative Medicine and Cellular Longevity

Self Cite

View full text Add to dashboard Cite

The response to strenuous exercise was investigated by reactive oxygen species (ROS) production, oxidative damage, thiol redox status, and inflammation assessments in 32 enrolled triathlon athletes (41:9 ± 7:9 yrs) during Ironman® (IR), or half Ironman® (HIR) competition. In biological samples, inflammatory cytokines, aminothiols (glutathione (GSH), homocysteine (Hcy), cysteine (Cys), and cysteinylglycine (CysGly)), creatinine and neopterin, oxidative stress (OxS) biomarkers (protein carbonyl (PC), thiobarbituric acid-reactive substances (TBARS)), and ROS were assessed. Thirteen HIR and fourteen IR athletes finished the race. Postrace, ROS (HIR +20%; IR +28%; p < 0:0001), TBARS (HIR +57%; IR +101%), PC (HIR +101%; IR +130%) and urinary neopterin (HIR +19%, IR +27%) significantly (range p < 0:05-0.0001) increased. Moreover, HIR showed an increase in total Cys +28%, while IR showed total aminothiols, Cys, Hcy, CysGly, and GSH increase by +48, +30, +58, and +158%, respectively (range p < 0:05-0.0001). ROS production was significantly correlated with TBARS and PC (R 2 = 0:38 and R 2 = 0:40; p < 0:0001) and aminothiols levels (range R 2 = 0:17-0.47; range p < 0:01-0.0001). In particular, ROS was directly correlated with the athletes' age (R 2 = 0:19; p < 0:05), with ultraendurance years of training (R 2 = 0:18; p < 0:05) and the days/week training activity (R 2 = 0:16; p < 0:05). Finally, the days/week training activity (hours/in the last 2 weeks) was found inversely correlated with the IL-6 postrace (R 2 = -0:21; p < 0:01). A strenuous performance, the Ironman® distance triathlon competition, alters the oxidant/antioxidant balance through a great OxS response that is directly correlated to the inflammatory parameters; furthermore, the obtained data suggest that an appropriate training time has to be selected in order to achieve the lowest ROS production and IL-6 concentration at the same time.

show abstract

A Quantitative Method to Monitor Reactive Oxygen Species Production by Electron Paramagnetic Resonance in Physiological and Pathological Conditions

Cited by 66 publications

References 32 publications

Oxidative stress response to acute hypobaric hypoxia and its association with indirect measurement of increased intracranial pressure: a field study

Oxidative stress response to acute hypobaric hypoxia and its association with indirect measurement of increased intracranial pressure: a field study

Reactive oxygen species and evaluation of the quality of carcasses and beef meat from cold storage slaughterhouses located in the Federal District and Surroundings (Brazil)

Acute Effects of Triathlon Race on Oxidative Stress Biomarkers

Contact Info

Product

Resources

About